LOCUS       V040313                 8722 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040313
VERSION     V040313
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3378
                     /label="TTLL6(NM_001130918)"
                     /note="TTLL6(NM_001130918)"
                     /gene="TTLL6"
     CDS             3385..3474
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    3498..4086
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4152..4200
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4402..4830
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4844..5173
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5240..6034
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6210..6331
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6380..6396)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6404..6420
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6428..6458)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6473..6494
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6782..7370)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7541..8401)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8402..8506)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggg agcgttactc
      721 cttcatcctt cgaggagggg gccggcaggt gtggttgcaa gctggactag cagcccagcg
      781 gggcgagacg ggggagtagg aattgcgggg gcctggtatt tccccagagc ctcctcccag
      841 gccagggaga tgccacagtg cccgactttg gaaagccagg aaggggaaaa ctccgaagag
      901 aagggggaca gttccaaaga agatccaaaa gaaaccgtcg cgctggcttt tgtgagagag
      961 aacccagggg cacaaaacgg acttcagaat gcccagcagc aaggcaagaa gaagaggaag
     1021 aaaaagagat tggtgatcaa tctatccagc tgccggtatg agagtgtgcg cagggctgcc
     1081 caacagtacg gctttagaga gggaggggaa gacgatgact ggactctcta ttggacagat
     1141 tactcagtgt cactggagcg ggtgatggaa atgaaaagtt accagaagat caatcacttc
     1201 cccgggatga gtgaaatctg ccggaaggac ttgctggcca ggaacatgag ccgcatgtta
     1261 aagatgttcc ctaaagattt ccgctttttc cctaggacct ggtgtcttcc tgctgactgg
     1321 ggagatttgc agacctacag caggtcaaga aaaaataaga catacatttg taagccggat
     1381 tcgggctgcc aagggaaagg tatattcatc acccggacag tgaaagaaat caaaccaggg
     1441 gaggatatga tctgtcagct gtatatttca aagcccttta tcattgatgg gtttaagttt
     1501 gacctacgga tttatgtact ggtgacatcc tgtgaccctc tcaggatttt tgtgtacaat
     1561 gaaggactgg cccgctttgc gacgacctct tactcccgcc cttgcacaga caacctggat
     1621 gatatctgca tgcacctgac taattattcc attaataagc acagttcaaa tttcagtcga
     1681 gatgcacact ctggcagtaa gaggaagctc tccaccttca gtgcatactt ggaggaccac
     1741 agctacaacg tggagcagat atggagggat attgaggacg tcatcatcaa gaccctcatc
     1801 tcggcccacc ccatcatcag gcataactac cacacctgct tccccaacca cacactcaac
     1861 agcgcctgct ttgagatcct gggctttgac attttgttgg accacaaact caaaccctgg
     1921 ctgctggagg tcaaccactc tccaagcttc tccaccgact ctcggttgga taaagaggtg
     1981 aaagatggtc tgctgtatga caccttagtc ctgatcaacc tggaaagctg tgacaagaag
     2041 aaagtcttgg aggaggagag acaacggggg cagttcctgc agcagtgttg ttctcgggag
     2101 atgaggattg aggaagccaa gggtttccgg gccgtgcagt taaagaaaac tgaaacgtat
     2161 gagaaggaaa actgtggagg gttccgactg atttatccca gtctgaattc ggagaagtat
     2221 gagaagtttt tccaggacaa caactccctc ttccagaata ctgttgcttc cagggctcgg
     2281 gaggagtatg cccggcaact gatccaggag ctgagactaa aacgggagaa aaagcccttc
     2341 caaatgaaga agaaggtaga gatgcagggg gaatcggcag gcgagcaagt gagaaagaag
     2401 ggcatgaggg gctggcaaca gaaacaacag cagaaagaca aggccgccac ccaagcctcc
     2461 aaacagtaca tccagccatt gacattagta tcctacacac ctgacttgct cttgagtgtc
     2521 agaggtgaaa ggaaaaatga aacagacagc agcctcaacc aggaggctcc cacggaggag
     2581 gccagctctg ttttccccaa gctgacgtct gcgaagccct tcagttctct acccgatctg
     2641 aggaatatca atctcagcag ctcgaagttg gagcccagta aacccaactt cagcatcaag
     2701 gaggccaagt ctgcctctgc agtgaacgta ttcactggca ctgtgcactt aacctccgta
     2761 gaaaccaccc cagaatccac cacccaactc tcaatctccc caaagtctcc gccaaccctg
     2821 gctgtgaccg ccagctctga gtacagtggc ccagagacgg acagggtggt atcctttaaa
     2881 tgcaagaagc agcagacccc tccacactta acccagaaga aaatgttaaa atcttttctg
     2941 cccacaaaat ccaagagctt ctgggagagt ccgaacacaa actggacttt gctaaagagt
     3001 gacatgaaca agccacattt gatatccgag ctactcacca agcttcaact gagtgggaag
     3061 ctctccttct tcccagctca ctacaacccc aagctgggga tgaataacct gtcacaaaac
     3121 ccctccctgc ctggggagtg ccactcccgc agtgacagct ctggcgagaa gaggcagctg
     3181 gatgtgtcct ccctcctctt gcagagtcct cagagctata atgttactct gagggacctg
     3241 ctggtgattg ccactccagc ccaactggat ccaaggcctt gtagaagcca cgcaagtgct
     3301 atgagggacc catgtatgca ggatcaagaa gcatacagcc attgcctgat ctctggccaa
     3361 aaaggatgtg agaggagcct cgaggaacaa aaactaatat cagaggaaga tttggaacag
     3421 aaactaatca gtgaagaaga tttagagcaa aagttaattt ccgaggagga cttatagtga
     3481 gggcccgata tctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     3541 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     3601 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3661 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     3721 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     3781 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3841 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     3901 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     3961 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4021 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4081 ccccgcctgg aaacggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     4141 ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc
     4201 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     4261 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     4321 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcagat ctgcgcagct
     4381 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4441 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4501 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca
     4561 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4621 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4681 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4741 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg
     4801 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     4861 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4921 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4981 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     5041 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5101 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5161 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     5221 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     5281 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5341 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5401 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5461 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5521 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5581 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5641 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5701 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5761 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     5821 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     5881 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     5941 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6001 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga
     6061 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     6121 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     6181 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     6241 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6301 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     6361 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6421 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6481 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6541 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6601 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6661 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6721 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6781 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6841 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6901 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6961 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7021 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7081 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7141 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7201 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     7261 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7321 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7381 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7441 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7501 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7561 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7621 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7681 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7741 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7801 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7861 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7921 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7981 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8041 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8101 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8161 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8221 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8281 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8341 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8401 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8461 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8521 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt cgactctcag
     8581 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     8641 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     8701 ttgcatgaag aatctgctta gg
//
