LOCUS       V040665                 9892 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040665
VERSION     V040665
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..4548
                     /label="TTLL5(NM_015072)"
                     /note="TTLL5(NM_015072)"
                     /gene="TTLL5"
     CDS             4555..4644
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    4668..5256
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    5322..5370
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5572..6000
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6014..6343
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6410..7204
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7380..7501
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7550..7566)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7574..7590
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7598..7628)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7643..7664
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7952..8540)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8711..9571)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9572..9676)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgcc aatcgtgatg
      721 gcccgggacc tggaggaaac agcatcatcc tcagaggatg aggaggtcat aagtcaagag
      781 gatcatccat gcatcatgtg gactggaggc tgcaggagaa ttccagtttt ggtattccat
      841 gccgacgcta ttcttacaaa ggacaacaat attagagtaa ttggagaacg ttatcatttg
      901 tcttataaga ttgtacgaac ggacagtcgc ctagtacgca gcattctgac agcccatgga
      961 tttcatgaag ttcacccaag cagcactgac tataacctaa tgtggacagg atcccacctg
     1021 aagcccttct tactgcgcac cctctctgaa gcacaaaaag ttaatcactt tcccaggtct
     1081 tatgaactta cccggaagga ccgactgtac aaaaacatta ttcgaatgca gcatacacat
     1141 ggattcaagg cttttcacat cctcccccag accttcctcc tgccagctga gtacgcggaa
     1201 ttttgtaatt catattcgaa ggaccgggga ccttggatag taaaaccagt ggcatcttca
     1261 agggggcggg gcgtctacct gatcaacaat ccaaaccaga tctccctgga agagaacatt
     1321 ttggtctccc gttacattaa caaccccctg ctcatagatg atttcaagtt tgacgtgcgc
     1381 ctctatgtgc tcgtgacttc ctatgatcct cttgtcatct atctctatga agaaggattg
     1441 gctaggtttg caactgtgcg atatgatcaa ggagccaaga acattcggaa ccagttcatg
     1501 catctgacaa actacagtgt caacaagaaa agtggagatt acgtcagttg tgacgatcca
     1561 gaagtggagg attatggaaa caaatggagc atgagtgcta tgcttaggta cctgaaacaa
     1621 gaaggcagag atacaaccgc attgatggcc catgtagaag acctgatcat taagactata
     1681 atctctgctg aactagctat tgctactgcc tgtaaaacct ttgttcctca tcgcagcagt
     1741 tgttttgaac tctatggctt tgacgtgctc atagattcta ctctgaagcc atggttgttg
     1801 gaagtgaatc tctctccttc tttggcctgt gatgcgcctc tggacctaaa gattaaagcc
     1861 agtatgattt cagatatgtt cactgttgta ggatttgtgt gccaagatcc tgcccagcgg
     1921 gcatcaactc ggccaattta tcccaccttt gagtcttcca ggcgaaaccc tttccagaaa
     1981 cctcagcgtt gccgtccact ctctgccagt gatgcggaaa tgaaaaacct cgtgggctca
     2041 gcccgggaga aagggccagg gaagttgggt ggttctgtgc ttggtctgtc aatggaggag
     2101 atcaaagttt tacgaagggt gaaggaggag aatgatcggc gaggtggatt tattcgcata
     2161 tttcctacat ctgagacatg ggaaatatat gggtcctacc tcgagcataa gacctcaatg
     2221 aactatatgc tggcaacacg cctcttccag gacagaatga ctgctgatgg agcgccagaa
     2281 ttgaagatag agagtctgaa ttcaaaggcc aagctgcatg ctgcacttta cgagaggaag
     2341 ctcctgtctc tggaggtgcg aaaacgtaga cgacggagta gcagattgag ggcaatgagg
     2401 ccaaaatacc cagtgattac ccaaccagct gaaatgaatg ttaaaactga gacagagagt
     2461 gaagaggagg aagaagtcgc attagataat gaagatgaag aacaggaggc ttcccaggag
     2521 gagtctgcag gatttcttag agaaaatcaa gccaaatata caccctcatt gacagctttg
     2581 gtagaaaata cacccaaaga aaattccatg aaagttcgtg aatggaataa taaaggtgga
     2641 cactgctgca aacttgagac tcaggagcta gagcctaaat ttaacctgat gcagattctt
     2701 caagataatg gcaatcttag caaaatgcag gcccgaatag cattctctgc ctatctccag
     2761 catgttcaaa ttcgcctgat gaaagacagt ggcggtcaga cgttcagtgc cagttgggct
     2821 gccaaagagg atgaacagat ggagctggtt gttcgtttcc tcaagcgagc atcaaataac
     2881 ctccagcatt cactgaggat ggtattaccc agtcgacgat tggcacttct ggaacgcaga
     2941 agaatcctgg cccaccagct gggtgacttt atcattgtat acaacaagga aacagaacaa
     3001 atggctgaaa agaaatcaaa gaagaaagtt gaggaagaag aggaagatgg ggtgaatatg
     3061 gaaaactttc aggagttcat cagacaagca agtgaggctg aactggagga ggtgttgact
     3121 ttttataccc aaaagaacaa gtctgctagt gtcttcctgg ggactcactc taaaatttct
     3181 aagaacaaca acaattattc tgatagtggg gcaaaaggtg atcaccctga gactataatg
     3241 gaagaagtga aaataaagcc acctaaacag caacagacga cagaaattca ttctgataaa
     3301 ttatctcgat ttaccacttc agcagaaaaa gaggcaaaat tagtttatag caattcctcc
     3361 tctggtccta ctgctactct gcagaaaatt cccaacaccc atttgtcatc tgttacaacc
     3421 tctgacctct ctccagggcc ttgccaccat tcttctttat ctcaaattcc ttcagctatc
     3481 cccagcatgc ctcaccagcc aacaatttta ctgaacacag tctctgccag tgcttctccc
     3541 tgcctacatc ccggggcaca gaacatccca agccctactg gcctgccacg ctgtcgatca
     3601 ggaagtcaca ccattggtcc cttttcttcc ttccaaagtg ctgcacacat ctatagccag
     3661 aaactgtctc gtccctcttc agcaaaggca ggatcgtgct atctaaacaa gcatcattca
     3721 ggaatagcca aaacacaaaa agagggagaa gatgcttctt tatatagcaa acggtacaac
     3781 caaagtatgg ttacagctga acttcagcgg ctagctgaga agcaggcagc gagacagtat
     3841 tctccatcca gccacatcaa cctcctcacc caacaggtaa caaacctgaa tttggcaact
     3901 ggcatcataa acagaagcag tgcttcagct cccccaaccc tccgacccat catcagtcct
     3961 agtggcccga catggtctac acagtcagac ccccaagctc ccgagaatca ctccagctct
     4021 cctggaagca ggagcctgca gacaggggga tttgcctggg aaggagaagt agaaaacaac
     4081 gtgtacagcc aggctacagg ggtggtcccc cagcacaagt atcaccccac agcaggcagc
     4141 tatcagcttc aatttgccct gcagcaactt gaacaacaaa aacttcagtc ccggcagctc
     4201 ctggaccaga gtcgagcccg gcaccaggca atctttggca gccagacact acctaactcc
     4261 aatttatgga caatgaataa tggtgcaggt tgtagaattt ccagtgccac agctagtggc
     4321 cagaagccaa ccactctgcc acaaaaagtg gtaccacctc caagttcttg cgcctccctg
     4381 gttcccaaac ccccacccaa ccacgaacaa gtgctcagaa gggcaacatc ccagaaagct
     4441 tccaaagggt cctccgcgga agggcagctg aatggactcc agagcagcct taaccctgca
     4501 gcctttgtgc ccatcaccag ctctacagat cctgctcaca ctaaaatact cgaggaacaa
     4561 aaactaatat cagaggaaga tttggaacag aaactaatca gtgaagaaga tttagagcaa
     4621 aagttaattt ccgaggagga cttatagtga gggcccgata tctcgacaat caacctctgg
     4681 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4741 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     4801 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     4861 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     4921 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     4981 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5041 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     5101 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5161 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5221 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aaacggggga ggctaactga
     5281 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     5341 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     5401 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     5461 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     5521 caggccctgc catagcagat ctgcgcagct ggggctctag ggggtatccc cacgcgccct
     5581 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5641 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5701 gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac
     5761 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5821 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5881 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5941 tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6001 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     6061 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     6121 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     6181 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6241 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     6301 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     6361 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     6421 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     6481 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     6541 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     6601 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     6661 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     6721 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     6781 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     6841 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     6901 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     6961 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     7021 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     7081 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     7141 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     7201 ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     7261 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     7321 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     7381 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7441 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7501 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7561 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7621 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7681 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7741 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     8161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     8401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8701 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8761 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8821 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8881 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8941 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9001 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9061 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9121 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9181 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9241 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9301 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9361 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9421 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9481 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9541 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9601 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9661 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     9721 atctcccgat cccctatggt cgactctcag tacaatctgc tctgatgccg catagttaag
     9781 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     9841 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gg
//
