LOCUS       V039728                 8311 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039728
VERSION     V039728
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2970
                     /label="TRIM56(NM_030961)"
                     /note="TRIM56(NM_030961)"
                     /gene="TRIM56"
     CDS             2977..3003
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3007..3033
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3037..3063
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3087..3675
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3741..3789
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3991..4419
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4433..4762
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4829..5623
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5799..5920
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5969..5985)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5993..6009
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6017..6047)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6062..6083
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6371..6959)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7130..7990)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7991..8095)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggt ttcccacggg
      721 tcctcgccct ccctcctgga ggccctgagc agcgacttcc tggcctgtaa aatctgcctg
      781 gagcagctgc gggcacccaa gacactgccc tgcctgcata cctactgcca agactgcctg
      841 gcacagctgg cggatggcgg ccgcgtccgc tgccccgagt gccgcgagac agtgcctgtg
      901 ccgcccgagg gtgtggcctc cttcaagacc aacttcttcg tcaatgggct gctggacctg
      961 gtgaaggccc gggcctgtgg agacctgcgt gccgggaagc cagcctgtgc cctgtgtccc
     1021 ctggtgggtg gcaccagcac cggggggccg gccacggccc ggtgcctgga ctgtgccgat
     1081 gacttgtgcc aggcctgtgc cgacgggcac cgctgcaccc gccagaccca cacccaccgc
     1141 gtggtggacc tggtgggcta cagggccggg tggtatgatg aggaggcccg ggagcgccaa
     1201 gcggcccagt gtccccagca ccccggggag gcactgcgct tcctgtgcca gccctgctca
     1261 cagttgctgt gcagagagtg ccgcctagac ccccacctgg accacccctg cctgcctctg
     1321 gctgaagctg tgcgtgcccg gaggccgggc ctggagggac tgctggccgg tgtggacaat
     1381 aacctggtgg agctggaggc agcgcggagg gtggagaagg aggcgctagc ccggctgcgg
     1441 gagcaggcgg cccgggtggg gactcaggtg gaggaggcgg ctgagggcgt cctccgggcc
     1501 ctgctggccc agaagcagga ggtgctgggg cagctacgag cccacgtgga ggctgccgaa
     1561 gaagctgctc gggagaggct ggcggagctt gagggccggg agcaggtggc cagggccgca
     1621 gccgccttcg cccgccgggt actcagcctg gggcgagagg ccgagatcct ctccctggaa
     1681 ggggcgatcg cacagcggct caggcagctg cagggctgcc cctgggcacc aggcccggcc
     1741 ccctgcctgc tcccacagct ggagctccat cctgggctcc tggacaagaa ctgccacctt
     1801 cttcggctgt cctttgagga gcagcagccc cagaaggatg gtgggaaaga cggagctggt
     1861 acccagggag gtgaggagag ccagagccgg agggaggatg agccgaagac tgagagacag
     1921 ggtggagtcc agccccaggc tggagatgga gcccagaccc caaaagagga aaaagcccag
     1981 acaacccgag aagagggagc ccagaccttg gaggaggaca gggcccagac accccacgag
     2041 gatggaggac cccagcccca caggggtggc agacccaaca agaagaaaaa gttcaaaggc
     2101 aggctcaagt caatttcccg ggagcccagc ccagccctgg ggccgaatct ggacggctct
     2161 ggcctcctcc ccagacccat cttttactgc agtttcccca cgcggatgcc tggagacaag
     2221 cggtcccccc ggatcaccgg gctctgtccc ttcggtcccc gggagatcct ggtggcggat
     2281 gagcagaacc gggcactgaa acgcttctcc ctcaacggcg actacaaggg caccgtgccg
     2341 gtccctgagg gctgctcccc ttgcagcgtg gccgccctgc agagcgcggt ggccttctcc
     2401 gctagcgcac ggctctatct catcaacccc aacggcgaag tgcagtggcg cagggccctg
     2461 agcctctccc aggccagcca cgcggtggcg gcactgccta gcggggaccg cgtggctgtc
     2521 agcgtggcgg gccacgtgga ggtgtacaat atggaaggca gcctggccac ccggttcatt
     2581 cctggaggca aggccagccg gggcctgcgg gcgctggtgt ttctgaccac cagcccccag
     2641 gggcatttcg tggggtcgga ctggcagcag aatagtgtgg taatctgtga tgggctgggc
     2701 caggtggttg gggagtacaa ggggccaggc ctgcatggct gccagccggg ctccgtgtct
     2761 gtggataaga agggctacat ctttctgacc cttcgagaag tcaacaaggt ggtgatcctg
     2821 gacccgaagg ggtccctcct tggagacttc ctgacagcct accacggcct ggaaaagccc
     2881 cgggttacca ccatggtgga tggcaggtac ctggtcgtgt ccctcagtaa cgggaccatc
     2941 cacatctttc gggtccgttc tccggacagt ctcgagtacc catacgacgt acctgattat
     3001 gcaacttatc cttatgatgt acctgattat gctagctatc cttatgatgt cccagactac
     3061 gcatagtgag ggcccgatat ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     3121 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     3181 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     3241 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     3301 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     3361 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     3421 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     3481 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     3541 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     3601 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     3661 gggccgcctc cccgcctgga aacgggggag gctaactgaa acacggaagg agacaatacc
     3721 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
     3781 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     3841 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     3901 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc
     3961 tgcgcagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     4021 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4081 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4141 atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4201 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4261 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4321 accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt
     4381 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     4441 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4501 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4561 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4621 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4681 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     4741 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     4801 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     4861 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     4921 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     4981 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     5041 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     5101 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     5161 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     5221 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     5281 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     5341 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     5401 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     5461 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     5521 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     5581 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     5641 gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     5701 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5761 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     5821 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5881 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     5941 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6001 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6061 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6121 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6181 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6241 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6301 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6361 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6421 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6481 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6541 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     6601 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6661 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6721 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6781 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     6841 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6901 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6961 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7021 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7081 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7141 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7201 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7261 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7321 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7381 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7441 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7501 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7561 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7621 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7681 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7741 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7801 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7861 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7921 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7981 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8041 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8101 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtc
     8161 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     8221 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     8281 gaccgacaat tgcatgaaga atctgcttag g
//
