LOCUS       V026799                 6339 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026799
VERSION     V026799
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1055..2554
                     /label="TRIM4(NM_033017)"
                     /note="TRIM4(NM_033017)"
                     /gene="TRIM4"
     CDS             2558..2587
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     primer_bind     complement(2596..2612)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2623..3245
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3394..3982)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4311..4358
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4590..5384)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5419..5776)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5778..5882)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5909..6337
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgcggc gcgccagatc tcaagcttgc caccatggaa gctgaggaca tccaggagga
     1081 gttgacctgc cccatctgcc tggactattt ccaggacccg gtgtccatcg agtgcggcca
     1141 caacttctgc cgcggctgcc tgcaccgcaa ctgggcgccg ggcggcggcc cgttcccctg
     1201 ccccgaatgt cggcacccat cggcgcccgc cgcgctgcga cccaactggg ccctggccag
     1261 gctgactgag aagacgcagc gccggcgcct gggccccgtg cccccgggcc tgtgcggccg
     1321 ccactgggag ccgctgcggc tcttctgcga ggacgaccag cggccagtgt gcctggtgtg
     1381 cagggagtcc caggagcacc agactcacgc catggcaccc atcgacgagg ccttcgagag
     1441 ctaccggaca ggtaactttg acatccacgt ggatgaatgg aagagaagac taattaggct
     1501 gctcttgtac cattttaagc aggaggagaa acttcttaag tctcagcgta atctcgtggc
     1561 caagatgaag aaagtcatgc atttacagga tgtagaagtg aagaacgcca cacagtggaa
     1621 ggataagata aagagtcagc gaatgagaat cagcacggag ttttcaaagc tgcacaactt
     1681 cctggttgaa gaagaggacc tgtttcttca gagattgaac aaagaagaag aagagacgaa
     1741 gaagaagctg aatgagaaca cgttaaaact caatcaaact atcgcttcat tgaagaagct
     1801 catcttagag gtgggggaga agagccaggc tcccaccctg gagctgcttc agaatccaaa
     1861 agaagtgttg accaggagtg agatccagga tgtgaactat tctcttgaag ctgtaaaggt
     1921 gaagacagtg tgccagatac cattgatgaa ggaaatgcta aagcgattcc aagtggctgt
     1981 aaacctagct gaagacacag ctcatcccaa actcgtcttc tcccaggaag ggagatacgt
     2041 gaaaaataca gcatcagcca gttcttggcc agtgttttct tcagcatgga actactttgc
     2101 tggatggagg aatcctcaga agactgcttt tgtagagaga tttcagcact taccctgtgt
     2161 tctgggaaaa aacgttttca cctcagggaa acattactgg gaagttgaga gtagagatag
     2221 tctggaggtt gctgttgggg tgtgtcggga ggacgtcatg ggaattactg atcgttcaaa
     2281 aatgtcccca gatgtgggca tctgggcgat ttattggagt gctgctggct attggccctt
     2341 gataggcttc cctggaactc ccacccagca agagccagct ctccaccgag tgggggttta
     2401 cctggatcgt gggactggga atgtctcctt ctacagcgct gtggacggag tgcacctgca
     2461 caccttttct tgttcttctg tctcacgcct ccggccattt ttttggttga gtccattagc
     2521 atctttagtc attccaccag tgactgatag gaaactcgag cagaaactca tctcagaaga
     2581 ggatctgtag ccgcggtcat agctgtttcc tgaacagatc ccgggtggca tccctgtgac
     2641 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     2701 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     2761 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     2821 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     2881 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     2941 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     3001 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     3061 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttaaa ataactatac
     3121 cagcaggagg acgtccagac acagcatagg ctacctggcc atgcccaacc ggtgggacat
     3181 ttgagttgct tgcttggcac tgtcctctca tgcgttgggt ccactcagta gatgcctgtt
     3241 gaattgggta cgcggccagc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3301 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3361 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3421 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3481 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3541 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3601 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3661 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3721 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3781 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3841 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3901 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3961 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4021 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4081 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaacc
     4141 tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
     4201 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
     4261 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
     4321 cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg
     4381 tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga actccagcat
     4441 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     4501 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     4561 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     4621 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     4681 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcgatcc
     4741 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     4801 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc
     4861 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     4921 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     4981 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     5041 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     5101 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     5161 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca
     5221 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     5281 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     5341 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     5401 cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     5461 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     5521 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     5581 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     5641 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     5701 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
     5761 gttctttccg cctcaggact cttccttttt caatattatt gaagcattta tcagggttat
     5821 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5881 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     5941 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6001 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6061 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6121 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6181 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6241 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6301 aaaaatgagc tgatttaaca aaaatttaac gcgaatttt
//
