LOCUS V027752 7471 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027752 VERSION V027752 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..2130 /label="TRIM21(NM_003141)" /note="TRIM21(NM_003141)" /gene="TRIM21" CDS 2137..2163 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2167..2193 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2197..2223 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 2247..2835 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2901..2949 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3151..3579 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3593..3922 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3989..4783 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4959..5080 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5129..5145) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5153..5169 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5177..5207) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5222..5243 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5531..6119) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6290..7150) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7151..7255) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc ttcagcagca 721 cgcttgacaa tgatgtggga ggaggtcaca tgccctatct gcctggaccc cttcgtggag 781 cctgtgagca tcgagtgtgg ccacagcttc tgccaggaat gcatctctca ggttgggaaa 841 ggtgggggca gcgtctgtcc tgtgtgccgg cagcgctttc tgctcaagaa tctccggccc 901 aatcgacagc tagccaacat ggtgaacaac cttaaagaaa tcagccagga ggccagagag 961 ggcacacagg gggaacggtg tgcagtgcat ggagagagac ttcacctgtt ctgtgagaaa 1021 gatgggaagg ccctttgctg ggtatgtgcc cagtctcgga aacaccgtga ccacgccatg 1081 gtccctcttg aggaggctgc acaggagtac caggagaagc tccaggtggc attaggggaa 1141 ctgagaagaa agcaggagtt ggctgagaag ttggaagtgg aaattgcaat aaagagagca 1201 gactggaaga aaacagtgga aacacagaaa tctaggattc acgcagagtt tgtgcagcaa 1261 aaaaacttcc tggttgaaga agaacagagg cagctgcagg agctggagaa ggatgagagg 1321 gagcagctga gaatcctggg ggagaaagag gccaagctgg cccagcagag ccaggcccta 1381 caggagctca tctcagagct agatcgaagg tgccacagct cagcactgga actgctgcag 1441 gaggtgataa ttgtcctgga aaggagtgag tcctggaacc tgaaggacct ggatattacc 1501 tctccagaac tcaggagtgt gtgccatgtg ccagggctga agaagatgct gaggacatgt 1561 gcagtccaca tcactctgga tccagacaca gccaatccgt ggctgatact ttcagaagat 1621 cggagacaag tgaggcttgg agacacccag cagagcatac ctggaaatga agagagattt 1681 gatagttatc ctatggtcct gggtgcccag cactttcact ctggaaaaca ttactgggag 1741 gtagatgtga caggaaagga ggcctgggac ctgggtgtct gcagagactc tgtgcgcagg 1801 aaggggcact ttttgcttag ttccaagagt ggcttctgga caatttggtt gtggaacaaa 1861 caaaaatatg aggctggcac ctacccccag actcccctcc accttcaggt gcctccatgc 1921 caagttggga ttttcctgga ctatgaggct ggcatggtct ccttctacaa catcactgac 1981 catggctccc tcatctactc cttctctgaa tgtgccttta caggacctct gcggcccttc 2041 ttcagtcctg gtttcaatga tggaggaaaa aacacagccc ctctaaccct ctgtccactg 2101 aatattggat cacaaggatc cactgactat ctcgagtacc catacgacgt acctgattat 2161 gcaacttatc cttatgatgt acctgattat gctagctatc cttatgatgt cccagactac 2221 gcatagtgag ggcccgatat ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat 2281 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 2341 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 2401 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 2461 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 2521 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 2581 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 2641 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 2701 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 2761 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 2821 gggccgcctc cccgcctgga aacgggggag gctaactgaa acacggaagg agacaatacc 2881 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt 2941 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga 3001 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt 3061 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc 3121 tgcgcagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 3181 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 3241 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 3301 atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 3361 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 3421 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 3481 accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt 3541 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca 3601 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 3661 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 3721 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 3781 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 3841 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 3901 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 3961 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 4021 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 4081 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 4141 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 4201 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 4261 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 4321 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 4381 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 4441 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 4501 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 4561 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 4621 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 4681 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 4741 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 4801 gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 4861 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 4921 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata 4981 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 5041 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga 5101 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 5161 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 5221 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 5281 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 5341 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 5401 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 5461 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 5521 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 5581 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 5641 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 5701 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg 5761 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 5821 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 5881 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 5941 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 6001 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 6061 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 6121 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 6181 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 6241 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 6301 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 6361 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 6421 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 6481 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 6541 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 6601 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 6661 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 6721 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 6781 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 6841 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 6901 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 6961 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 7021 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 7081 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 7141 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 7201 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 7261 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtc 7321 gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg 7381 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt 7441 gaccgacaat tgcatgaaga atctgcttag g //