LOCUS       V026687                 8182 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026687
VERSION     V026687
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2841
                     /label="TICAM1(NM_182919)"
                     /note="TICAM1(NM_182919)"
                     /gene="TICAM1"
     CDS             2848..2874
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2878..2904
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2908..2934
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    2958..3546
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3612..3660
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3862..4290
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4304..4633
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4700..5494
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5670..5791
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5840..5856)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5864..5880
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5888..5918)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5933..5954
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6242..6830)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7001..7861)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7862..7966)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc ctgcacaggc
      721 ccatcacttc ctagcgcctt cgacattcta ggtgcagcag gccaggacaa gctcttgtat
      781 ctgaagcaca aactgaagac cccacgccca ggctgccagg ggcaggacct cctgcatgcc
      841 atggttctcc tgaagctggg ccaggaaact gaggccagga tctctctaga ggcattgaag
      901 gccgatgcgg tggcccggct ggtggcccgc cagtgggctg gcgtggacag caccgaggac
      961 ccagaggagc ccccagatgt gtcctgggct gtggcccgct tgtaccacct gctggctgag
     1021 gagaagctgt gccccgcctc gctgcgggac gtggcctacc aggaagccgt ccgcaccctc
     1081 agctccaggg acgaccaccg gctgggggaa cttcaggatg aggcccgaaa ccggtgtggg
     1141 tgggacattg ctggggatcc agggagcatc cggacgctcc agtccaatct gggctgcctc
     1201 ccaccatcct cggctttgcc ctctgggacc aggagcctcc cacgccccat tgacggtgtt
     1261 tcggactgga gccaagggtg ctccctgcga tccactggca gccctgcctc cctggccagc
     1321 aacttggaaa tcagccagtc ccctaccatg cccttcctca gcctgcaccg cagcccacat
     1381 gggcccagca agctctgtga cgacccccag gccagcttgg tgcccgagcc tgtccccggt
     1441 ggctgccagg agcctgagga gatgagctgg ccgccatcgg gggagattgc cagcccacca
     1501 gagctgccaa gcagcccacc tcctgggctt cccgaagtgg ccccagatgc aacctccact
     1561 ggcctccctg atacccccgc agctccagaa accagcacca actacccagt ggagtgcacc
     1621 gaggggtctg caggccccca gtctctcccc ttgcctattc tggagccggt caaaaacccc
     1681 tgctctgtca aagaccagac gccactccaa ctttctgtag aagataccac ctctccaaat
     1741 accaagccgt gcccacctac tcccaccacc ccagaaacat cccctcctcc tcctcctcct
     1801 cctccttcat ctactccttg ttcagctcac ctgaccccct cctccctgtt cccttcctcc
     1861 ctggaatcat catcggaaca gaaattctat aactttgtga tcctccacgc cagggcagac
     1921 gaacacatcg ccctgcgggt tcgggagaag ctggaggccc ttggcgtgcc cgacggggcc
     1981 accttctgcg aggatttcca ggtgccgggg cgcggggagc tgagctgcct gcaggacgcc
     2041 atagaccact cagctttcat catcctactt ctcacctcca acttcgactg tcgcctgagc
     2101 ctgcaccagg tgaaccaagc catgatgagc aacctcacgc gacaggggtc gccagactgt
     2161 gtcatcccct tcctgcccct ggagagctcc ccggcccagc tcagctccga cacggccagc
     2221 ctgctctccg ggctggtgcg gctggacgaa cactcccaga tcttcgccag gaaggtggcc
     2281 aacaccttca agccccacag gcttcaggcc cgaaaggcca tgtggaggaa ggaacaggac
     2341 acccgagccc tgcgggaaca gagccaacac ctggacggtg agcggatgca ggcggcggca
     2401 ctgaacgcag cctactcagc ctacctccag agctacttgt cctaccaggc acagatggag
     2461 cagctccagg tggcttttgg gagccacatg tcatttggga ctggggcgcc ctatggggct
     2521 cgaatgccct ttgggggcca ggtgcccctg ggagccccgc caccctttcc cacttggccg
     2581 gggtgcccgc agccgccacc cctgcacgca tggcaggctg gcaccccccc accgccctcc
     2641 ccacagccag cagcctttcc acagtcactg cccttcccgc agtccccagc cttccctacg
     2701 gcctcacccg caccccctca gagcccaggg ctgcaacccc tcattatcca ccacgcacag
     2761 atggtacagc tggggctgaa caaccacatg tggaaccaga gagggtccca ggcgcccgag
     2821 gacaagacgc aggaggcaga actcgagtac ccatacgacg tacctgatta tgcaacttat
     2881 ccttatgatg tacctgatta tgctagctat ccttatgatg tcccagacta cgcatagtga
     2941 gggcccgata tctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     3001 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     3061 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3121 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     3181 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     3241 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3301 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     3361 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     3421 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     3481 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     3541 ccccgcctgg aaacggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     3601 ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc
     3661 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     3721 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     3781 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcagat ctgcgcagct
     3841 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3901 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3961 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca
     4021 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4081 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4141 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4201 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg
     4261 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     4321 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4381 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4441 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4501 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4561 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4621 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     4681 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     4741 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     4801 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     4861 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     4921 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     4981 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5041 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5101 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5161 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5221 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     5281 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     5341 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     5401 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     5461 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga
     5521 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     5581 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5641 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     5701 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5761 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     5821 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     5881 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     5941 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6001 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6061 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6121 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6181 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6241 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6301 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6361 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6421 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6481 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6541 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6601 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6661 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     6721 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6781 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6841 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6901 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6961 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7021 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7081 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7141 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7201 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7261 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7321 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7381 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7441 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7501 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7561 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7621 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7681 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7741 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7801 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7861 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7921 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7981 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt cgactctcag
     8041 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     8101 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     8161 ttgcatgaag aatctgctta gg
//
