LOCUS       V029953                 6171 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029953
VERSION     V029953
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2318
                     /label="Smad9(NM_019483)"
                     /note="Smad9(NM_019483)"
                     /gene="Smad9"
     CDS             2337..2366
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2385..2408
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2428..2444)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2455..3077
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3226..3814)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4143..4190
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4422..5216)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5251..5608)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5610..5714)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5741..6169
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gcaccccagc acccccatca gctccctctt ctccttcacc agccccgcag
     1081 tgaagcggct gctgggctgg aagcagggag atgaagagga gaagtgggca gagaaggcgg
     1141 tggactcttt ggtgaagaag ttaaagaaga agaaaggcgc catggatgaa ctggagaggg
     1201 cgctgagctg cccgggtcag cctagcaagt gtgtcaccat cccacggtcc ctcgatggac
     1261 gcctccaggt gtcccaccga aaggggctgc cccacgtcat ctactgccgc gtgtggcgct
     1321 ggccagacct gcagtcccat catgagctga agcccttgga gtgctgtgag ttcccgttcg
     1381 gctccaagca gaaggaggtc tgcatcaacc cataccatta ccgcagagtg gagaccccag
     1441 ttctgcctcc agtgctggta ccaagacaca gcgagtacaa ccctcagctc agcctcctgg
     1501 ccaagttccg aagtgcctcg ctgcacagcg aacccctcat gccgcacaac gccacctacc
     1561 ctgactcttt ccagcagtct ctctgtccgg caccgccctc ctcgccaggc catgtgtttc
     1621 cgcagtctcc atgccccacc agctacccgc actcccccgg aagtccttcc gagtcagaca
     1681 gtccctatca acactcagac ttccggccag tttgctacga ggaaccccag cactggtgtt
     1741 ctgttgccta ctacgaacta aacaaccggg tcggagagac tttccaggcg tcctcgcgga
     1801 gcgtgctcat agacggcttc accgaccctt ccaataacag gaataggttc tgccttgggc
     1861 ttctctcaaa tgtaaacaga aactcgacca tagaaaacac caggaggcac attggaaagg
     1921 gtgtgcattt gtactacgtt gggggcgagg tgtatgcgga gtgcgtgagc gacagcagca
     1981 tctttgtcca gagccggaac tgcaactacc agcacggctt ccacccggcc accgtctgca
     2041 agatccccag cggctgcagc ctcaaggtct tcaacaacca gctcttcgcc cagctgctcg
     2101 cccagtccgt gcaccacggc tttgaagtgg tgtatgagct gacgaagatg tgcacgattc
     2161 ggatgagctt tgtgaagggc tggggagcag agtatcatcg ccaggatgtc acgagcaccc
     2221 cctgctggat cgagatccat cttcatggac cgctgcagtg gttggataag gtgctcactc
     2281 agatgggctc cccacacaac cctatctctt cagtgtctac gcgtacgcgg ccgctcgagc
     2341 agaaactcat ctcagaagag gatctggcag caaatgatat cctggattac aaggatgacg
     2401 acgataaggt ttaaacggcc ggccgcggtc atagctgttt cctgaacaga tcccgggtgg
     2461 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     2521 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     2581 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     2641 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     2701 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     2761 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     2821 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     2881 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta
     2941 aaataactat accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa
     3001 ccggtgggac atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag
     3061 tagatgcctg ttgaattggg tacgcggcca gcggcgagcg gtatcagctc actcaaaggc
     3121 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3181 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3241 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3301 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3361 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3421 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3481 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3541 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3601 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3661 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3721 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3781 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3841 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3901 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3961 atatgagtaa cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg
     4021 gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc
     4081 ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt
     4141 atgaacaaac gacccaacac cgtgcgtttt attctgtctt tttattgccg tcatagcgcg
     4201 ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa
     4261 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
     4321 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt
     4381 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
     4441 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     4501 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     4561 tgatagcgat ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     4621 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
     4681 ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     4741 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     4801 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     4861 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     4921 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     4981 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt
     5041 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     5101 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     5161 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
     5221 aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc
     5281 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     5341 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     5401 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     5461 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     5521 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     5581 attccacagc tggttctttc cgcctcagga ctcttccttt ttcaatatta ttgaagcatt
     5641 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5701 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     5761 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5821 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5881 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5941 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6001 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6061 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6121 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt t
//
