LOCUS       V030984                10344 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030984
VERSION     V030984
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..6482
                     /label="Sis(NM_001081137)"
                     /note="Sis(NM_001081137)"
                     /gene="Sis"
     CDS             6510..6539
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             6558..6581
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(6601..6617)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    6628..7250
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(7399..7987)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    8316..8363
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(8595..9389)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(9424..9781)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(9783..9887)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      9914..10342
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcaaagaag aaattcagtg gattagaaat ctctctgatt gttctcttca
     1081 tcatagtaac gataatagct atcgctcttg ttgtggtttt ggctacaaag gtgcctgctg
     1141 tggaagaagt aaaaagtcca acttcaaccc ctagtcctgg aaggtgtccg cctgagcaag
     1201 gtgaacctct caatgagaga ataaactgca ttccagaaca gcatccaaca aaggcaaaat
     1261 gtgaagagcg tggctgttgc tggcgtccat ggaacaacac tattattcct tggtgcttct
     1321 ttgcagataa ccatggctat actgctgcgt cagtcacgaa tgacaacagt ggacttaaag
     1381 ccacactgag cagaatccct tctcccactc tatttggaga agacattaaa agcgtcctcc
     1441 tcacgactca gagtcagacc cgtaatcgtt tccggttcaa gctcacagat ccaaataaca
     1501 agagatacga agtgccacat cagtttgtaa aagatggaaa tggaatccca gcagctgaca
     1561 cactgtatga tgtcaaggtc tcagaaaacc cgttcagcat caaagtcatc aggaaaagca
     1621 acaacaaagt cttgtttgat accagtattg gtcctctggt gtactcaaac caatatttgc
     1681 agatttcaac aagacttcca agtgaatata tttatggctt tggagaacat attcataaga
     1741 gatttcgtca tgacttatac tggaaaacat ggccaatctt tactcgagac gagattcctg
     1801 gtgataataa tcataatctc tatgggcatc agacattctt catgggcatt gaagataact
     1861 ctggcaagtc ctatggtgtt ttccttatga acagcaatgc aatggaggtt ttcatccagc
     1921 caacaccaat aattacttac agagttactg gtggcgtatt agacttttat atatttcttg
     1981 gagacacacc agaacaagtt gttcaacaat atcaagagct cattggacga ccagcaatgc
     2041 cagcatattg gaaccttggc tttcaactga gtcgctggaa ttacgtgtct ttagataaag
     2101 tgaaagaagt agtaagaaga aaccgggaag ctggcatccc atatgatgca caagtcactg
     2161 atattgacta catggaagac aaaaaggact ttacttatga tgaagtggca tttaaaggac
     2221 ttcctgagtt tgctcaggat ttacataacc atggacaaaa atatattatc atcctggacc
     2281 ctgcaatctc tataaacaaa cgtgcaaatg gtgccgaata tcaaacatat gtgaggggga
     2341 atgagcaaaa tgtttgggtg aaagagtcag atggaaccac atcacttatt ggagaggtat
     2401 ggccaggatt aacagtgtac ccagatttta ctaatcccag aacctgggag tggtgggcaa
     2461 atgaatgcaa tcttttccat cagcaagtgg aatatgatgg actctggatt gacatgaatg
     2521 aagtttccag ctttatccac ggttctcaga aaggatgtgc tcccaatcta ctgaattacc
     2581 ctccttttac tcccggtatt cttgacaaaa tcatgtactc caaaacgctt tgcatggatg
     2641 ctgtgcagca ctgggggaat cagtatgatg tacacagcct gtatggatac agcatggcta
     2701 tagccacaga gaaagctgtg gaaaaagttt ttcctaataa gagaagcttc atccttaccc
     2761 ggtcaacatt tgctggttcg ggacaccatg ctgctcattg gcttggagac aacacagcct
     2821 cctgggagca gatggagtgg tccatcactg gaatgctgga gttcggcatg ttcggtatgc
     2881 ctttggtagg ggcggacatc tgtggatttt tggctaacac cacagaagaa ctctgcagaa
     2941 gatggatgca actgggagcc ttttatccat tttctagaaa ccacaatgct gaaggctatg
     3001 cggaacaaga tcctgccttt tggggagcag actctctttt ggtaaacaca tcaaggcact
     3061 acctaaccat ccgctacact ttacttccct tcctctacac tctgttctat agagcccacg
     3121 cgtttggtga gacagttgca aggccatttc ttcatgagtt ttatgaagac ccaaacagct
     3181 ggattgagga cacccagttc ctgtggggcc ctgcactgct gatcactcct gttttgagac
     3241 cagaaacaaa atacgtgagt gcatatattc ctgatgctac ctggtatgat tatgaaacag
     3301 gtgaaaaaag accatggaga aaacaaaggg ttgacatgta ccttccagaa gataaaatag
     3361 gattacatct tagaggaggt tatatcatcc ctacccagca acctgatgta acaacaacag
     3421 caagccgaaa gaatcctcta ggccttattg ttgcattaga tgaaaaccag gcagcaaaag
     3481 gagaactctt ctgggatgat ggtgaatcta aagatactat agaaaagaag atctatatat
     3541 tatatgagtt ttcagtttct aataataact tgattgttaa ctgcacacat tcatcatatc
     3601 cagagggaaa tacccttgtg tttaaaacaa tcaaagtcct tgggctgagc gccactgtca
     3661 cagcggttac agtgggtgaa aatgatcagc aaatgaaccc tcatcttgct ttcacttttg
     3721 atgctttcaa taagattctc tcaattacag atctcacatt caacctcggc aaaaccttta
     3781 tagttagatg gacgacgcaa agtttctcag acaatgagaa attcacttgt tatccagatg
     3841 taggcacagc tactgagaaa acttgcgtag agagaggctg catatgggaa ccggtgagcg
     3901 ggctcgccaa tgtgcctcct tgctactttc cttcgaacca caatccctat ttgttaactt
     3961 caactcaaaa attggcaacg ggcataacag cagaacttca gttgaatcct gcaagtgcta
     4021 gaattaagct tccatctaat cccatctcca ctcttcgcgt ggaagtaaaa tatcacaaaa
     4081 atgacatgct acagtttaag atttacgatg cacatcataa gagatacgaa gttcctgtac
     4141 cactaaatat tccagacacc cctacaagct ctgaggaaaa tagactctat gatgtagaaa
     4201 tcaaggaaaa tccttttggc atccaggttc gaaggagaag cactggaaag cttatctggg
     4261 attcttgcct acctggattt gccttcaatg atcaattcat tcagatatct acacgtctgc
     4321 catcacaata tttatatggt tttggggaag cggaacacac agcattcaag cgaaatttga
     4381 attggcatac ttgggggatg ttcacaagag atcaacctcc tggatataaa cttaattcct
     4441 atggattcca tccgtattac atggctttgg aggatgaggg caatgctcat ggagtcctgt
     4501 tacagaacag caatggaatg gatgttacat tccagccaac acctgctcta acctaccgca
     4561 caattggtgg aatcttggat ttttacatgt ttttgggacc aaccccagaa ggtgcaacaa
     4621 agcaatatca tgaagtaatt ggctttccag tcatgccacc ttactgggct ctgggattcc
     4681 aactatgtcg ttatggatac agaaatactt cagagattga gcaactgtac aatgacatga
     4741 aagctgctca aatcccctat gatgttcagt atacagacat caattatatg gaaagacaac
     4801 tagacttcac aattggtgaa cgattcaaga ctcttcctca gtttgttgaa aaaataagaa
     4861 aagagggaat gaaatatatt gttatactgg atccagcaat ttcaggaaat gaaacccagc
     4921 cttatcctgc cttcgaaaga ggaatacaga aagatgtttt tgtcaaatgg cctaacacaa
     4981 atgacatttg ctgggcaaag gtttggccag atttacctaa tattacaata gatgagacta
     5041 taacagaaga tgaagctgtt aacgcttcca gagcacatgt agcttttcca gacttcttta
     5101 ggaattccac atcagagtgg tggacaagag aaatttatga cttctacaat gaaaaaatga
     5161 agtttgatgg cttgtggatt gacatgaatg agccttccag ctttgtaaat ggaaccgtta
     5221 caaataaatg cagaaatgat acactaaatt atccacctta ttttccagaa ctcacaaaaa
     5281 gaaatgaggg gttacatttc cgaaccatgt gcatggaaac agaacatatc cttagtgatg
     5341 gatcatcagt tttgcattat gatgtgcaca atttatatgg atggtcacaa gttaaaccca
     5401 ctctggatgc attacggaac accactggcc ttcgagggat agtcatttct cggtccacat
     5461 atcccacagc tggtcgatgg ggaggacact ggttgggaga caactatgca aactgggaga
     5521 atttggaaaa atcactcatt ggtatgctgg aatttaatct ttttggaata ccatatgttg
     5581 gagcagatat ctgtggtttc tttaatgact cagaatacca cctctgtgct cgttggatgc
     5641 aagttggagc cttttatcca tattcaagaa atcacaacat tcaatttact agaaggcaag
     5701 atcctgtttc ctggaatgaa acttttgctc aaatgtcaaa gaaagtttta gaaatcagat
     5761 acacattgct gccctatttc tacacacaga tgcatgaagc ccatattcat ggtggaactg
     5821 tcatcagacc cctaatgcat gagttctttg atgataaaga aacctgggaa atatacaagc
     5881 agttcctgtg gggtccagct tttatggtga ctcctgtcat tggacctttc cagacagctg
     5941 tgaatggcta tgttcctaag gctcgatggt ttgactacca tacaggggaa gacataaagg
     6001 tcagaggaaa acttcaaaca ttttcagctc cttttgatac aataaatcta catgtccggg
     6061 gaggttatat tctcccatgt caagaaccgg cacagaacac atattacagt cgacagaaat
     6121 acatgaaact catcgtagct gcagatgaca accaaacagc aaaaggaact ctattctggg
     6181 atgatggaga gtcgatagac acatatgaaa aaaatcaata taccttgata gaattcaatt
     6241 taaatcagaa aaatttaaca agtactgtat tgaaagatgg atatagaaac aaaagtgaga
     6301 tgaaacttgg aagtatctat gtatggggaa aaggaacaac cagtatcagt caggttaatc
     6361 tgacttatgg tgggaatgaa caacagcttt catttactca agacgaagct aaggagatac
     6421 taacaattga cctgaaaaat gtaactgtta ctcttgatga accaatacaa atcagctggt
     6481 caagcggacc gacgcgtacg cggccgctcg agcagaaact catctcagaa gaggatctgg
     6541 cagcaaatga tatcctggat tacaaggatg acgacgataa ggtttaaacg gccggccgcg
     6601 gtcatagctg tttcctgaac agatcccggg tggcatccct gtgacccctc cccagtgcct
     6661 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     6721 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     6781 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     6841 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     6901 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     6961 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     7021 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     7081 accactgctc ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc
     7141 cagacacagc ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt
     7201 ggcactgtcc tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg
     7261 ccagcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7321 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7381 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7441 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7501 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7561 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7621 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7681 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7741 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7801 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7861 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7921 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7981 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8041 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8101 ttaaaaatga agttttaaat caatctaaag tatatatgag taacctgagg ctatggcagg
     8161 gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg gggggtgggg
     8221 tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg gggtatcgac
     8281 agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa caccgtgcgt
     8341 tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg
     8401 tgtttcagtt agcctccccc tagggtgggc gaagaactcc agcatgagat ccccgcgctg
     8461 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     8521 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     8581 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     8641 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     8701 tcagcaatat cacgggtagc caacgctatg tcctgatagc gatccgccac acccagccgg
     8761 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     8821 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac
     8881 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     8941 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     9001 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     9061 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     9121 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     9181 agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc
     9241 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     9301 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     9361 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatcg
     9421 atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     9481 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     9541 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     9601 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     9661 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     9721 gcctggggac tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca
     9781 ggactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9841 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9901 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9961 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    10021 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    10081 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    10141 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    10201 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    10261 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    10321 taacaaaaat ttaacgcgaa tttt
//
