LOCUS       V030979                 6876 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030979
VERSION     V030979
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3023
                     /label="Sh3kbp1(NM_001135727)"
                     /note="Sh3kbp1(NM_001135727)"
                     /gene="Sh3kbp1"
     CDS             3042..3071
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3090..3113
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3133..3149)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3160..3782
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3931..4519)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4848..4895
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5127..5921)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5956..6313)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6315..6419)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6446..6874
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggtggaggcc atagtggagt ttgattacca ggcccagcat gatgatgagc
     1081 tgacaatcag cgtgggtgag gtcatcacca acatcaggaa ggaggatgga ggctggtggg
     1141 agggacagat caacggcagg agaggtttgt tccctgacaa ctttgtaaga gaaataaaga
     1201 aagatatgaa gaaagatctt ctctctaaca aagctccaga gaaacccatg cacgatgtat
     1261 ccagtggaaa cgctctgctt tcttctgaaa ccattttaag aaccaataaa cgaggagaac
     1321 gacggagacg ccgatgccag gtggcattca gctacctgcc ccagaatgac gatgagctgg
     1381 agctgaaagt cggggacatc atagaggttg taggagaggt agaggaagga tggtgggaag
     1441 gtgtgctcaa tgggaagact ggaatgtttc cttccaactt catcaaggag ctgtcagggg
     1501 agtcggatga gcttggcatc tcccaggatg agcagctttc caagtcaagc ttaagagaaa
     1561 ccacaggctc cgagagtgat ggaggcgact ccagcagtac caagtccgaa ggtgccaacg
     1621 ggaccatggc aactgcagcc atccagccca agaaagttaa aggagtgggg tttggagaca
     1681 ttttcaaaga caagccaatt aaactaagac caaggtcgat tgaagtggaa aatgacttcc
     1741 tgcccgtcga aaagacaatt gggaagaagt tacctccagc tacatcaact ccagacccat
     1801 cgaagacgga gatggacagc aggacaaaga ccaaggatta ctgcaaagta atatttccat
     1861 atgaggcaca gaatgatgat gaattgacaa tcaaggaagg agatatagtg actctcatca
     1921 ataaggactg catcgacgta ggctggtggg aaggagaact caatggcaga cgaggcgtgt
     1981 ttcctgacaa cttcgtgaag ttacttccat cggactttga caaggaaggg aatagaccca
     2041 agaaaccgcc acctccatca gctcctgtcg tcaaacaagg ggcaggtacc acagagagaa
     2101 aacatgaaat taaaaaaata cctcctgaaa gaccagaaac ccttccaaac agaacagaag
     2161 agaaagaaag accagagaga gagccaaaac tggatttaca gaagccttca gttcctgcca
     2221 tcccaccaaa aaagcctcgg ccacctaaga ccaattctct gaacagacct ggtgcattgc
     2281 ccccaagaag acctgagcga ccagtgggcc cactgacaca caccaggggt gacagtccaa
     2341 agattgactt ggcaggcagc gcactctctg gcatcctgga taaggacctc tcagaccgca
     2401 gcaatgatat tgacttagaa ggttttgact ctgtgatatc atctactgag aaactcagcc
     2461 acccaaccac aagcagacca aaagctacag gaagacggcc tccatcccag tcactcacat
     2521 cttcttccct ttcaagcccc gatatctttg actccccaag tcccgaagaa gataaagagg
     2581 aacacatttc gcttgcgcac agaggaatag acgtgtcaaa gaagacttcc aagactgtta
     2641 ccatatccca agtgtctgat aacaagacat ccctgccacc caagccaggg accatggcag
     2701 ctgccagcag tgggccagct tctctctctt cagtggcatc ctcacccatg tcatcctctt
     2761 tgggaacagc tggacagaga gccagttctc catctctgtt cagcacagaa ggaaagccaa
     2821 agatggagcc agcagtgagc agccaggctg ctatcgagga gcttaagatg caagtccgtg
     2881 agctgaggac catcattgag accatgaagg accagcagaa acgtgagatt aagcagttac
     2941 tgtcagaatt ggatgaagag aaaaagatcc ggctccggtt gcagatggaa gtgaacgaca
     3001 taaagaaagc tcttcaatca aagacgcgta cgcggccgct cgagcagaaa ctcatctcag
     3061 aagaggatct ggcagcaaat gatatcctgg attacaagga tgacgacgat aaggtttaaa
     3121 cggccggccg cggtcatagc tgtttcctga acagatcccg ggtggcatcc ctgtgacccc
     3181 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     3241 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     3301 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     3361 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     3421 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     3481 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     3541 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     3601 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttaaaata actataccag
     3661 caggaggacg tccagacaca gcataggcta cctggccatg cccaaccggt gggacatttg
     3721 agttgcttgc ttggcactgt cctctcatgc gttgggtcca ctcagtagat gcctgttgaa
     3781 ttgggtacgc ggccagcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3841 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3901 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3961 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4021 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4081 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4141 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4201 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4261 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4321 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4381 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4441 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4501 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4561 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4621 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga
     4681 ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact
     4741 tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg
     4801 tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc
     4861 aacaccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat
     4921 tgtctccttc cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag
     4981 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     5041 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     5101 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     5161 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     5221 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcgatccgcc
     5281 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     5341 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     5401 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     5461 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     5521 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     5581 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     5641 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     5701 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     5761 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     5821 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     5881 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     5941 gtctcttgat cgatctttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     6001 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     6061 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     6121 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     6181 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     6241 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     6301 ctttccgcct caggactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6361 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6421 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6481 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6541 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6601 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6661 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6721 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6781 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6841 aatgagctga tttaacaaaa atttaacgcg aatttt
//
