LOCUS       V030055                 6387 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030055
VERSION     V030055
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2534
                     /label="Sgsh(NM_018822)"
                     /note="Sgsh(NM_018822)"
                     /gene="Sgsh"
     CDS             2553..2582
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2601..2624
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2644..2660)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2671..3293
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3442..4030)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4359..4406
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4638..5432)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5467..5824)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5826..5930)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5957..6385
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gcactgcccg ggactggcct gctgcacaat tctgttggtc ctgggactct
     1081 gcggcgcgca ctcgcgcaac gtgctactga tagttgcgga tgacggaggc tttgagagtg
     1141 gtgtatacaa caacactgcc atcgccaccc ctcacctgga tgccctgtcc cgccacagcc
     1201 ttatcttccg taacgccttc acgtctgtca gcagctgttc cccgagccgt gccagcctcc
     1261 tcaccggcct gccccagcat cagaatggca tgtatgggct gcaccaggat gtgcatcact
     1321 tcaactcttt tgacaaggta cagagccttc cgctgctgct caaccaggcc ggagtgcgca
     1381 caggcatcat tgggaagaag cacgtgggtc cggagacggt gtatcccttt gactttgcat
     1441 tcacagagga gaacagctct gtgatgcagg tggggcggaa catcactaga attaagcaac
     1501 tggtccagaa atttctgcag actcaggatg acaggccctt cttcctgtac gtggctttcc
     1561 atgacccgca ccgctgtggg cactctcagc cccagtatgg aaccttctgt gagaagtttg
     1621 gcaacggaga gagtggaatg gggtacattc cagactggac accccagatc tacgaccctc
     1681 aggatgtgat ggtgccctac tttgtcccgg acacaccagc agcccgagca gacctagctg
     1741 ctcagtacac caccatcggg cggatggacc aaggggtagg tctggtgctc caggagctgc
     1801 gaggtgctgg tgtgctgaac gacaccctca tcatcttcac atctgacaat ggtatccctt
     1861 tccccagcgg caggaccaac ctgtactggc ccggtacagc cgagcctttg ctggtgtcat
     1921 ctccagagca cccacagcgc tggggccagg tcagcgacgc ctacgtgagc cttctagacc
     1981 tcacccctac catcctggac tggttctcca tcccgtaccc cagctatgcc atctttggct
     2041 caaagacgat ccagctcaca ggccgatccc tcctgccggc gctggaggca gagccccttt
     2101 gggccactgt cttcagcagc cagagccacc acgaggtcac catgtcctac ccgatgcgct
     2161 cggtgtacca ccagaacttc cgcctcattc acaacctgag cttcaagatg ccatttccca
     2221 tcgaccaaga tttctatgtc tcgccgacct tccaggacct cctgaaccga accaccacag
     2281 gccggcccac gggctggtac aaggacctcc accgttacta ctaccgggaa cgctgggaac
     2341 tctacgacat cagccgggac cctcgagaga cacggaacct ggccgctgac ccagacttgg
     2401 ctcaagtgct ggagatgctg aaagctcagc ttgtcaagtg gcaatgggag acacatgacc
     2461 cctgggtgtg cgccccagat ggagtcctgg aggaaaagct cacaccccag tgccgaccgc
     2521 ttcacaatga actcacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc
     2581 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc
     2641 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg
     2701 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     2761 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     2821 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     2881 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     2941 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc
     3001 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc
     3061 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg
     3121 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac
     3181 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg
     3241 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg
     3301 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3361 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3421 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3481 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3541 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3601 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3661 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3721 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3781 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3841 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3901 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3961 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4021 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4081 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4141 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc
     4201 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg
     4261 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc
     4321 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg
     4381 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt
     4441 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg
     4501 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     4561 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     4621 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     4681 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     4741 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc
     4801 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     4861 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg
     4921 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     4981 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     5041 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     5101 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     5161 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     5221 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg
     5281 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     5341 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     5401 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     5461 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     5521 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     5581 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     5641 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     5701 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     5761 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc
     5821 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5881 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5941 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6001 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6061 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6121 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6181 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6241 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6301 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6361 atttaacaaa aatttaacgc gaatttt
//
