LOCUS       V043624                13146 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043624
VERSION     V043624
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..7826
                     /label="Sec16a(NM_153125)"
                     /note="Sec16a(NM_153125)"
                     /gene="Sec16a"
     CDS             7833..7898
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    7922..8510
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    8576..8624
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8826..9254
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9268..9597
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             9664..10458
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    10634..10755
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10804..10820)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10828..10844
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10852..10882)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10897..10918
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11206..11794)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11965..12825)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12826..12930)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgca gccaccacct caggcagtcc
      781 cgtctggggt ggctggacca cccccagctg ggaatcctcg gagcatgttc tgggccaaca
      841 gcccttacag gaagccagcc aataatgcac cagtggctcc cataacacgc ccattgcagc
      901 cagtaacgga tccgtttgct tttaatagac agactcttca aaacacacca gtaggaagtt
      961 catccaaaag cagcctgccc aatttgcctg gcccagccct ctctgttttt tctcagtggc
     1021 ctggtttgcc tgtgactccc acaaatgctg gggatagctc cacaggattg catgagcctc
     1081 tgtcaggaac tctgtcacag cctagagcag atgccagtct atttcctcct gcatctaccc
     1141 cttcttcact ccctgggctg gaggtgagca ggaatgctga ggctgatccc agctcaggac
     1201 atgaagtcca gatgctgcca cattcagctc actacattcc aggagtgggt cctgagcagc
     1261 ctctcggggg ccaaatgaat gacagtgggt ccgggcctga ccaacccatg aataggcatg
     1321 ccccccatga tggtgccgtg acccacgcag catctccttt cctccctcag ccacagatgc
     1381 caggtcagtg ggggcctgca caaggaggcc ctcagccctc ctatcagcat cactcaccct
     1441 acctggaggg acctgttcag aacatggggc tccaggctgc cagcttgccc cattttcccc
     1501 ctccatctag tctacaccag ggacctggcc atgagtctca tgccccacaa acatttactc
     1561 cagcatcctt ggccagtggt gaagggaatg agatagttca ccagcaaagt aaaaatcacc
     1621 cattgagtag cttcccccct aaacacactt ttgagcagaa ttccagaatt gggaatatgt
     1681 gggcaagccc agagttgaag cagaatccgg gagtgaataa agagcatctg ctagaccctg
     1741 cccatgtcaa tcccttcact cagggaaata gtcctgaaaa ccaggcgcat catcccccag
     1801 tggcagcaac taaccatgcc ctgcaagaag cagcctctgg agctctctca atgttttttc
     1861 aaggggaaga gacagagaat gaggaaaatc tctcatctga aaaagcaggc ctcgataaga
     1921 gattgaactt ggatagcttt tcttctacct ccagactggg ccatccacca ccacctggag
     1981 ctagtggtgt ctaccaggcc tttcctagag gccctagcag tgaggctgcg caggaaggag
     2041 atgcgcaacc ttatttttcg cagtctgtgg gcgtccgtct tgacaaacag agcactgtgc
     2101 cccctgctaa tgatgcatgg ggtgatgttc cggggaccgg gacccgctgt gccagtgggc
     2161 cacagtgtga gaatgttgag aacttagaat ttgttcagaa tcaagaagtt ttgccccgtg
     2221 agaccctgag tgtggatccc ttccccctga gtgatcagat ccgatatggg ccccttcctg
     2281 ggccagctgc ctccaggcct gctactgtgg gcctcaccag aggtgggggg ctaaatcttg
     2341 aggccccaga cacaccactg caccctacac gacctgacag tgtgtcatcc agctacagca
     2401 gccatagcca cagaagtcct cctgggtcag ccagacccca agaattggtg ggcacattca
     2461 ttcagcaaga agttgggaaa cttgaagatg atacttcagg gagttttttt aagcaaattg
     2521 attcttctcc tgttggaggt gaaacagatg aggtgactgg gagccaaaat tgctgcagca
     2581 gtctgtccca gccttcaacc ccaagccctc caaaacctac aggagtattc caaacaagtg
     2641 caaacagttc ttttgagcca gtaaaatccc acttggttgg agtgaaacca gttgaagcag
     2701 atcgtgccaa catggtggtt gaagtgagag ggacccaata ctgtccgaag aagcgcaggg
     2761 cagctgttgc accacctgat gctacctcag gaaacctgga acagccacca gacaacatgg
     2821 agaccccgtg tgcacctcag gcctgccctt tgcctcttag caccactgga gaggctgggc
     2881 aactggtttc aaacacggca gggacacccc tggatactgt gcgcccagtg cctgataaga
     2941 ggccctcagc cagggcccag ggacctgtga agtgtgagag cccagcaaca actctgtggg
     3001 cacaaaatga gctgccagat tttggtggca atgtccttct agcccctgct gcccctgcac
     3061 tttatgttcc tgtaaaacca aaaccttcag aggttgttca tcatccagaa aagggaatgt
     3121 ctggacagaa ggcctggaaa caagggtctg tgccaccttt gcagaaccaa gaccctcctg
     3181 gtgcttctga gaaccttgag aaccctccca aggtaggaga agaggaggcc cttccagtgc
     3241 aggcaagttc tggttatgcg agtttgcttt cttcgccacc cactgaatct ttgcacaatc
     3301 aacctgtcct gattgctcag cctgatcaaa gctataattt ggctcagcct attaattttt
     3361 ctgtgtcctt actgaatcct aatgagaaga atcagtcctg gggagatgct gtggtggggg
     3421 agagatccat agtgagcaac aactgggctc tcggtggtga tcctgaagaa cgtgctgctc
     3481 tgtctggagt tccagccagt gctgtcactg gcgcgtcttt gcctagcagt atcccccaga
     3541 attgtgcccc tcaaggttcg gggtcttctg aaatgattgc tagccagtct gccagttggt
     3601 tggttcaaca gctatcccct caaacccctc aaagtccgca tccaaatgca gaaaagggcc
     3661 cttccgagtt tgttagtagc cctgctggca atacaagtgt gatgctagtt ccacctgcaa
     3721 gcagtacttt ggttcctaac agtaataagg caaagcactc tagtaaccag gaagaagctg
     3781 ttggcgccct agacttcaca ttaaatagaa ctttggaaaa tcctgtgaga atgtacagcc
     3841 catctccttc agatggccca gcttctcagc agcctcttcc caatcatccc agacaatctg
     3901 ggcctgggct gcataaccag gatcatttct accagcaggt aacaaaagat gctcaggacc
     3961 agcatagact agaaagagcc cagccagagc tggtgcctcc tcggccacag aattctcccc
     4021 aagtgcccca agcatcgtgt ccagagcctt caaatcctga aagcccaccc acacaaggac
     4081 agtctgaaag cttggcccag ccaccagcaa gtccagcttc agtcaacaca ggccagctgc
     4141 tgcctcagcc accacaggct tccagtgcat ccgttacatc taccaactcc agccaggcag
     4201 ctgtgcggtc agagcagctg tggctacacc caccacctcc taatactttt ggcccagcac
     4261 ctcaagactt ggcatcctac tactattaca gacccctgta tgatgcctac cagtctcagt
     4321 acccttcacc gtacccgtca gatcctggga cagcctcgct gtactaccag gatatgtatg
     4381 gcctgtatga gcctcgttac agaccctatg acagctcagc atctgcttat gccgagaacc
     4441 accgctattc tgagcctgag cggcccagct cccgtgcaag tcactattcc gaccagcttg
     4501 ctcccaggca aggatatcct gaaggatact ataattccaa gagtggatgg agcagtcaca
     4561 gtgattacta tgcaaattat tactctggcc agtatgatta tggagacccc agccgctggg
     4621 atcgttacta tgggtctcgc cttagggatc ctcgcacctg ggaccggagg tactggtatg
     4681 actctgaaca tgacccatac aggaaggacc actatgctta cagtgacagg cctgagaaat
     4741 gtgatgatca ctggaggtat gaccctcgct tcactgggag cttcgacgat gacgctgaga
     4801 tccacaggga cccctatgga gaagaagcag acagacgcag catccacagt gagcactcgg
     4861 cacggagcct gcgcagcact cacagtctgc ccagccgccg cagcagcctc agctcccatt
     4921 cacaccagag tcagatttac agaagccacc atgtgactgg cggttccttt gaggcccctc
     4981 atgccccagg ctcatttcat ggtgattatg cctatggcac atatgccagc aatttcagcg
     5041 gtgcccatgg ttttccagag tacagctacc ctgcagacac ctcctggcct gctgtggagc
     5101 aagttccatc aagaccgacc tctcctgaga agtttacggt gcctcatgtc tgtgccaggt
     5161 ttggtcctgg tggtcagctc cttaaagtga ttcccaacct gccttcagaa ggacagcctg
     5221 cactggtgga gatccacagc ttagagacct tgctgcagca cacacctgag caggaagaga
     5281 tgcgctcctt cccaggacct ctcggcaaag atgataccca caaagtagat gttattaact
     5341 ttgcacagaa caaagcaaca aaatgtttgc agaatgaaag tttaattgat aaagagtctg
     5401 caagtcttct ttggaaattc attattctgt tatgcagaca gaatgggact gttgtgggaa
     5461 cagacattgc agagcttttg ttacgggacc atagaactgt atggcttcct gggaagtcac
     5521 ccaatgaggc aaacctgata gattttacca atgaggctgt ggaacaagtg gaagaggagg
     5581 agtcagggga ggcccagctc tcatttctca cagacagtca gacagtgacc accagcgtgc
     5641 tggagaagga gactgagcgg ttccgtgagc tcttgctgta tggccggaag aaggatgctc
     5701 tggagtctgc catgaaaaat ggcttatggg gtcatgctct tctgcttgca agtaagatgg
     5761 acagccggac acatgcccgt gtcatgacca ggtttgccaa cagtcttcca atcaatgacc
     5821 ccctgcagac tgtctaccag ctcatgtctg gacggatgcc tgctgcatcc acgtgttgtg
     5881 gcgatgagaa gtggggagat tggaggccac atcttgcaat ggttttgtcc aacctgaaca
     5941 acaacatgga tgttgaatct aggaccatgg ctacgatggg tgatactcta gcatcaaaag
     6001 gcctgttgga tgctgcacac ttctgctacc tgatggccca ggttggattt ggggtttata
     6061 caaagaaaac tacaaaactt gtcttgatag gctcaaatca cagtttgccc tttttaaagt
     6121 ttgccaccaa tgaagccatt caaaggacgg aggcttatga gtatgctcag tcccttgggg
     6181 cacatacctg ttccttacct aacttccagg tgtttaaatt catctacttg tgccgcctgg
     6241 ctgaaatggg acttgccaca caggccttcc actactgcga agtaatagcc aagagtgtcc
     6301 tgacacagcc tggtgcatac tctccagtac tgattagcca gttgactcag atggcttccc
     6361 agttacgcct cttcgatcct caactgaagg agaagccaga agaggagtcc tttgtggagc
     6421 ctgcctggtt ggtgcagctg cagcacgtgg agaggcagat ccaggagggc actgtgctgt
     6481 ggagccagga tggaactgaa ccccagcagt gtcgcattac atcgggctct gaggtggagc
     6541 agtcagatgg ccctggactc aaccagcagg cagggccaca ggccgacaac cctctactga
     6601 tgccaagcac tgagcctttg atgcatggcg tgcaactgct gcccacagct cctcagacat
     6661 tgcctgatgg ccagcctgct cacctttcca gggtgccgat gttcccagtg ccaatgtctc
     6721 gtgggcccct ggagctgagt cctgcctatg gacccccagg atctgcactt ggcttcccag
     6781 aatcctccag atctgatcct gcagtgctgc atcctgggca ggccctgcca cccactacac
     6841 taagtctcca ggaaagtggg cttccacccc aggaggccaa aagtccagac ccagaaatgg
     6901 tgccacgggg ctcacctgtc agacactccc ctccggagct cagtcaagag gagtttggcg
     6961 agagcttcgc tgacccgggc tcttccagaa cagcacagga cttggaaacc tccccagtgt
     7021 gggatcttgg cagctccagt ttgacacgtg caccatcttt gacatctgat tctgaaggga
     7081 agaaacctgc acaggctgtc aaaaaggagc ccaaggagcc caagaagact gagtcctggt
     7141 tctctcgttg gctgcctgga aaaaaaagga cagaagctta tctaccagat gacaagaaca
     7201 aatcaattgt ttgggatgaa aagaaaaacc agtgggtgaa tttgaatgaa ccagaggagg
     7261 agaagaaggc tccaccccca cctccaacat cgttccccag ggttccccag gtggctccca
     7321 ctgggcctgc aggaccaccc acggcctccg tgaatgtgtt ttctagaaaa gcaggtgggt
     7381 ccagagctcg ctatgtggat gttctaaacc caagtggaac acagcggagt gaaccagctc
     7441 ttgctcctgc agatttcttt gctcctctcg ccccactgcc aattccttct aacttatttg
     7501 taccaaaccc agatgcagaa gagccacaac ctgcagatgg gactggctgt aggggacagg
     7561 caccagctgg gactcagtct aaggcagagt ccaccctgga acccaaggtg ggaagttcta
     7621 cagtatcagc ccctggacct gagctcttac cctccaaacc agatggctcc caaggaggag
     7681 aggctcctgg agaccattgt cctacagggg cccctcatgg tgggtctgtg cccttttaca
     7741 accctgctca gttggtgcag gcttctgtca cctcgggaaa ttcaaggcca gggaggattg
     7801 gccaaagaaa atatgcggca ttgaacggat ccgactacaa agaccatgac ggtgattata
     7861 aagatcatga catcgactac aaggatgacg atgacaagta gtgagggccc gatatctcga
     7921 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     7981 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     8041 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     8101 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     8161 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     8221 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     8281 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     8341 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     8401 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     8461 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaaacgg
     8521 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     8581 ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg
     8641 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     8701 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     8761 caacgtcggg gcggcaggcc ctgccatagc agatctgcgc agctggggct ctagggggta
     8821 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     8881 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     8941 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg
     9001 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     9061 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     9121 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     9181 tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     9241 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     9301 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     9361 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9421 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9481 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     9541 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9601 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     9661 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     9721 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     9781 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     9841 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     9901 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     9961 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
    10021 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
    10081 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
    10141 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
    10201 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
    10261 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
    10321 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
    10381 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
    10441 cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc
    10501 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    10561 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    10621 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10681 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10741 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
    10801 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    10861 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    10921 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    10981 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    11041 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    11101 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    11161 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    11221 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    11281 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    11341 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    11401 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    11461 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    11521 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    11581 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    11641 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    11701 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    11761 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    11821 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    11881 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    11941 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    12001 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    12061 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    12121 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    12181 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    12241 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    12301 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    12361 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    12421 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    12481 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    12541 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    12601 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    12661 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    12721 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    12781 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    12841 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    12901 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    12961 cgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
    13021 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
    13081 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
    13141 cttagg
//
