LOCUS V019092 8339 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019092 VERSION V019092 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 671..3010 /label="STRN(NM_003162)" /note="STRN(NM_003162)" /gene="STRN" CDS 3026..3091 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 3115..3703 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3769..3817 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4019..4447 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4461..4790 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4857..5651 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5827..5948 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5997..6013) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6021..6037 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6045..6075) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6090..6111 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6399..6987) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7158..8018) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8019..8123) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgccacc atggacgagc aggcgggtcc cggcgtcttc ttcagcaaca accacccggg 721 cgccggcggt gccaaggggc tcgggcctct ggcggaggct gccgcggccg gcgacggggc 781 ggctgcggcg ggggcggccc gagcccagta cagtctcccg gggatcctgc acttcctgca 841 gcacgagtgg gcccgcttcg aggtggagag agcccagtgg gaggtggagc gggcggagct 901 gcaggcccag attgccttcc tgcagggaga aaggaagggc caagaaaatt tgaagaagga 961 tcttgtgagg aggatcaaaa tgttggagta tgctcttaaa caggaaagag ccaaatacca 1021 caagttgaaa tacgggacag aattgaatca gggagatatg aagcctccaa gctatgattc 1081 tgatgaaggt aatgaaacag aagtgcagcc acaacaaaac agccagttaa tgtggaaaca 1141 aggtcgacaa ctactcagac agtatctaca ggaggtgggt tatacagata ctattctaga 1201 tgtgaaatct aaacgagtgc gagctttgtt gggcttttca agtgatgtca cggacaggga 1261 agatgacaaa aatcaggact cagttgtaaa tggcacagag gctgaagtta aagagacagc 1321 aatgattgca aaatctgagt taacagattc tgcctccgtg ctggataatt tcaaattcct 1381 tgaaagtgca gctgcagatt tcagtgatga agatgaagat gatgatgttg atggaagaga 1441 gaaaagcgtc attgatactt caacaattgt taggaaaaaa gcattgcctg acagcggtga 1501 agatcgagat acaaaagaag ctctaaagga gtttgacttc ttggttacat cagaggaagg 1561 agacaatgaa tctagaagtg caggcgatgg aacagactgg gaaaaggaag accagtgtct 1621 catgcctgaa gcctggaatg tggaccaggg agtaattacc aaactcaagg aacaatacaa 1681 aaaggagaga aaggggaaaa agggggtgaa gaggcccaat aggtcaaaac tacaagatat 1741 gcttgctaat ttgagagatg ttgatgaact tccttcattg cagccatctg tgggttcacc 1801 ttccagaccc agcagctcca ggcttcctga acatgaaatt aatagggcag atgaagtgga 1861 agcattgaca tttcctcctt cttctggaaa gtcattcatc atgggagcag atgaagccct 1921 tgaaagtgaa ctgggacttg gagaactagc aggccttacg gtggccaatg aagcagactc 1981 actaacttat gatatagcaa acaataaaga tgcattgagg aagacatgga accctaagtt 2041 tacattgaga agtcactttg atggcatccg agcccttgct ttccatccca ttgagcctgt 2101 tttgataaca gcatcagagg atcacacatt aaaaatgtgg aatttacaga aaacagcccc 2161 agccaaaaag agcacttctc ttgatgtaga acctatctat acattcagag cccataaagg 2221 tccagtgctt tgtgtggtaa tgagcagcaa tggtgagcag tgttacagtg gtggtactga 2281 tggactgatc cagggctgga ataccactaa tcccaacatc gacccctatg attcttatga 2341 tccttctgtt ttacgaggcc ctctgctagg ccacacggat gcagtctggg gtttggctta 2401 tagtgcagca catcagcgtt tgttgtcctg ttcagcagat ggcactctgc gtttatggaa 2461 tacaactgag gttgctccag cactaagtgt atttaatgat actaaagaac tgggaatccc 2521 tgcctctgtg gatctagtga gcagtgaccc gagccatatg gtagcatcat tcagcaaggg 2581 atatacaagc atttttaaca tggaaacaca acaacgcatt ctcactttag aatccaatgt 2641 agatacaaca gccaactctt cctgccaaat aaatagagtc atcagtcatc ctactcttcc 2701 gatcagcatc actgctcatg aagacaggca catcaaattc tatgataaca atacaggcaa 2761 actgatccac tcgatggtag cccacctaga agctgttaca agtttagcag ttgatcccaa 2821 tggcctttac ttgatgtctg gcagtcatga ctgttcaata cgtttatgga atctagaaag 2881 taagacgtgt atccaagaat tcacagctca tcgaaaaaag tttgaagaat cgattcatga 2941 tgtagctttc cacccatcca aatgctatat agccagtgct ggagctgacg cactggctaa 3001 agtctttgta atgcggccgc tcgaggacta caaagaccat gacggtgatt ataaagatca 3061 tgacatcgac tacaaggatg acgatgacaa gtagtgaggg cccgatatct cgacaatcaa 3121 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 3181 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 3241 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 3301 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 3361 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 3421 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 3481 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 3541 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 3601 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 3661 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaaa cgggggaggc 3721 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa 3781 gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag 3841 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct 3901 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc 3961 ggggcggcag gccctgccat agcagatctg cgcagctggg gctctagggg gtatccccac 4021 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 4081 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 4141 ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt 4201 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 4261 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 4321 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 4381 gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 4441 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 4501 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 4561 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 4621 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 4681 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 4741 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 4801 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga 4861 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 4921 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 4981 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 5041 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 5101 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 5161 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 5221 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 5281 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 5341 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg 5401 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 5461 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 5521 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 5581 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 5641 agttcttctg agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg 5701 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 5761 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 5821 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 5881 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 5941 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca 6001 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 6061 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 6121 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 6181 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 6241 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 6301 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 6361 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 6421 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 6481 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 6541 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca 6601 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 6661 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 6721 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 6781 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 6841 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 6901 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 6961 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 7021 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 7081 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 7141 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 7201 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 7261 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 7321 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 7381 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 7441 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 7501 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 7561 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 7621 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 7681 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 7741 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 7801 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 7861 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 7921 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 7981 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 8041 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 8101 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga 8161 tcgggagatc tcccgatccc ctatggtcga ctctcagtac aatctgctct gatgccgcat 8221 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 8281 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttagg //