LOCUS V035044 7956 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V035044 VERSION V035044 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 678..696 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 744..1451 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 1500..2636 /label="STING1(NM_198282)" /note="STING1(NM_198282)" /gene="STING1" CDS 2643..2708 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 2732..3320 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3386..3434 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3636..4064 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4078..4407 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4474..5268 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5444..5565 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5614..5630) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5638..5654 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5662..5692) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5707..5728 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6016..6604) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6775..7635) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7636..7740) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct 661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta 721 aacttaagct tggtaccgcc accatggtga gcaagggcga ggaggataac atggccatca 781 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg 841 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 901 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg 961 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 1021 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 1081 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 1141 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg 1201 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga 1261 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc 1321 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact 1381 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg 1441 agctgtacaa gtggatcgga ggagggggaa gcggtggagg cggtagtgag ctcggatcca 1501 tgccccactc cagcctgcat ccatccatcc cgtgtcccag gggtcacggg gcccagaagg 1561 cagccttggt tctgctgagt gcctgcctgg tgaccctttg ggggctagga gagccaccag 1621 agcacactct ccggtacctg gtgctccacc tagcctccct gcagctggga ctgctgttaa 1681 acggggtctg cagcctggct gaggagctgc gccacatcca ctccaggtac cggggcagct 1741 actggaggac tgtgcgggcc tgcctgggct gccccctccg ccgtggggcc ctgttgctgc 1801 tgtccatcta tttctactac tccctcccaa atgcggtcgg cccgcccttc acttggatgc 1861 ttgccctcct gggcctctcg caggcactga acatcctcct gggcctcaag ggcctggccc 1921 cagctgagat ctctgcagtg tgtgaaaaag ggaatttcaa cgtggcccat gggctggcat 1981 ggtcatatta catcggatat ctgcggctga tcctgccaga gctccaggcc cggattcgaa 2041 cttacaatca gcattacaac aacctgctac ggggtgcagt gagccagcgg ctgtatattc 2101 tcctcccatt ggactgtggg gtgcctgata acctgagtat ggctgacccc aacattcgct 2161 tcctggataa actgccccag cagaccggtg accatgctgg catcaaggat cgggtttaca 2221 gcaacagcat ctatgagctt ctggagaacg ggcagcgggc gggcacctgt gtcctggagt 2281 acgccacccc cttgcagact ttgtttgcca tgtcacaata cagtcaagct ggctttagcc 2341 gggaggatag gcttgagcag gccaaactct tctgccggac acttgaggac atcctggcag 2401 atgcccctga gtctcagaac aactgccgcc tcattgccta ccaggaacct gcagatgaca 2461 gcagcttctc gctgtcccag gaggttctcc ggcacctgcg gcaggaggaa aaggaagagg 2521 ttactgtggg cagcttgaag acctcagcgg tgcccagtac ctccacgatg tcccaagagc 2581 ctgagctcct catcagtgga atggaaaagc ccctccctct ccgcacggat ttctctctcg 2641 aggactacaa agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg 2701 atgacaagta gtgagggccc gatatctcga caatcaacct ctggattaca aaatttgtga 2761 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 2821 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 2881 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 2941 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 3001 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 3061 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 3121 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg 3181 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 3241 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc 3301 cctttgggcc gcctccccgc ctggaaacgg gggaggctaa ctgaaacacg gaaggagaca 3361 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt 3421 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc 3481 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc 3541 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc 3601 agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag 3661 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 3721 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 3781 tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 3841 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 3901 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 3961 actcaaccct atctcggtct attcttttga tttataaggg attttgggga tttcggccta 4021 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg 4081 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 4141 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 4201 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 4261 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 4321 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc 4381 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga 4441 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 4501 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 4561 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 4621 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg 4681 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 4741 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 4801 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 4861 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 4921 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 4981 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc 5041 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 5101 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 5161 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 5221 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 5281 ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac 5341 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 5401 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 5461 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 5521 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 5581 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 5641 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 5701 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 5761 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 5821 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 5881 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 5941 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 6001 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 6061 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 6121 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 6181 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt 6241 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 6301 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 6361 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 6421 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 6481 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 6541 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 6601 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 6661 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 6721 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 6781 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 6841 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 6901 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 6961 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 7021 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 7081 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 7141 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 7201 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 7261 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 7321 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 7381 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 7441 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 7501 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 7561 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 7621 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 7681 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 7741 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta 7801 tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct 7861 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa 7921 ggcttgaccg acaattgcat gaagaatctg cttagg //