LOCUS       V037972                 9580 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037972
VERSION     V037972
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..4236
                     /label="SREBF1(NM_001005291)"
                     /note="SREBF1(NM_001005291)"
                     /gene="SREBF1"
     CDS             4243..4332
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    4356..4944
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    5010..5058
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5260..5688
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5702..6031
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6098..6892
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7068..7189
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7238..7254)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7262..7278
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7286..7316)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7331..7352
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7640..8228)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8399..9259)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9260..9364)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgga cgagccaccc
      721 ttcagcgagg cggctttgga gcaggcgctg ggcgagccgt gcgatctgga cgcggcgctg
      781 ctgaccgaca tcgaaggtga agtcggcgcg gggaggggta gggccaacgg cctggacgcc
      841 ccaagggcgg gcgcagatcg cggagccatg gattgcactt tcgaagacat gcttcagctt
      901 atcaacaacc aagacagtga cttccctggc ctatttgacc caccctatgc tgggagtggg
      961 gcagggggca cagaccctgc cagccccgat accagctccc caggcagctt gtctccacct
     1021 cctgccacat tgagctcctc tcttgaagcc ttcctgagcg ggccgcaggc agcgccctca
     1081 cccctgtccc ctccccagcc tgcacccact ccattgaaga tgtacccgtc catgcccgct
     1141 ttctcccctg ggcctggtat caaggaagag tcagtgccac tgagcatcct gcagaccccc
     1201 accccacagc ccctgccagg ggccctcctg ccacagagct tcccagcccc agccccaccg
     1261 cagttcagct ccacccctgt gttaggctac cccagccctc cgggaggctt ctctacagga
     1321 agccctcccg ggaacaccca gcagccgctg cctggcctgc cactggcttc cccgccaggg
     1381 gtcccgcccg tctccttgca cacccaggtc cagagtgtgg tcccccagca gctactgaca
     1441 gtcacagctg cccccacggc agcccctgta acgaccactg tgacctcgca gatccagcag
     1501 gtcccggtcc tgctgcagcc ccacttcatc aaggcagact cgctgcttct gacagccatg
     1561 aagacagacg gagccactgt gaaggcggca ggtctcagtc ccctggtctc tggcaccact
     1621 gtgcagacag ggcctttgcc gaccctggtg agtggcggaa ccatcttggc aacagtccca
     1681 ctggtcgtag atgcggagaa gctgcctatc aaccggctcg cagctggcag caaggccccg
     1741 gcctctgccc agagccgtgg agagaagcgc acagcccaca acgccattga gaagcgctac
     1801 cgctcctcca tcaatgacaa aatcattgag ctcaaggatc tggtggtggg cactgaggca
     1861 aagctgaata aatctgctgt cttgcgcaag gccatcgact acattcgctt tctgcaacac
     1921 agcaaccaga aactcaagca ggagaaccta agtctgcgca ctgctgtcca caaaagcaaa
     1981 tctctgaagg atctggtgtc ggcctgtggc agtggaggga acacagacgt gctcatggag
     2041 ggcgtgaaga ctgaggtgga ggacacactg accccacccc cctcggatgc tggctcacct
     2101 ttccagagca gccccttgtc ccttggcagc aggggcagtg gcagcggtgg cagtggcagt
     2161 gactcggagc ctgacagccc agtctttgag gacagcaagg caaagccaga gcagcggccg
     2221 tctctgcaca gccggggcat gctggaccgc tcccgcctgg ccctgtgcac gctcgtcttc
     2281 ctctgcctgt cctgcaaccc cttggcctcc ttgctggggg cccgggggct tcccagcccc
     2341 tcagatacca ccagcgtcta ccatagccct gggcgcaacg tgctgggcac cgagagcaga
     2401 gatggccctg gctgggccca gtggctgctg cccccagtgg tctggctgct caatgggctg
     2461 ttggtgctcg tctccttggt gcttctcttt gtctacggtg agccagtcac acggccccac
     2521 tcaggccccg ccgtgtactt ctggaggcat cgcaagcagg ctgacctgga cctggcccgg
     2581 ggagactttg cccaggctgc ccagcagctg tggctggccc tgcgggcact gggccggccc
     2641 ctgcccacct cccacctgga cctggcttgt agcctcctct ggaacctcat ccgtcacctg
     2701 ctgcagcgtc tctgggtggg ccgctggctg gcaggccggg cagggggcct gcagcaggac
     2761 tgtgctctgc gagtggatgc tagcgccagc gcccgagacg cagccctggt ctaccataag
     2821 ctgcaccagc tgcacaccat ggggaagcac acaggcgggc acctcactgc caccaacctg
     2881 gcgctgagtg ccctgaacct ggcagagtgt gcaggggatg ccgtgtctgt ggcgacgctg
     2941 gccgagatct atgtggcggc tgcattgaga gtgaagacca gtctcccacg ggccttgcat
     3001 tttctgacac gcttcttcct gagcagtgcc cgccaggcct gcctggcaca gagtggctca
     3061 gtgcctcctg ccatgcagtg gctctgccac cccgtgggcc accgtttctt cgtggatggg
     3121 gactggtccg tgctcagtac cccatgggag agcctgtaca gcttggccgg gaacccagtg
     3181 gaccccctgg cccaggtgac tcagctattc cgggaacatc tcttagagcg agcactgaac
     3241 tgtgtgaccc agcccaaccc cagccctggg tcagctgatg gggacaagga attctcggat
     3301 gccctcgggt acctgcagct gctgaacagc tgttctgatg ctgcgggggc tcctgcctac
     3361 agcttctcca tcagttccag catggccacc accaccggcg tagacccggt ggccaagtgg
     3421 tgggcctctc tgacagctgt ggtgatccac tggctgcggc gggatgagga ggcggctgag
     3481 cggctgtgcc cgctggtgga gcacctgccc cgggtgctgc aggagtctga gagacccctg
     3541 cccagggcag ctctgcactc cttcaaggct gcccgggccc tgctgggctg tgccaaggca
     3601 gagtctggtc cagccagcct gaccatctgt gagaaggcca gtgggtacct gcaggacagc
     3661 ctggctacca caccagccag cagctccatt gacaaggccg tgcagctgtt cctgtgtgac
     3721 ctgcttcttg tggtgcgcac cagcctgtgg cggcagcagc agcccccggc cccggcccca
     3781 gcagcccagg gcaccagcag caggccccag gcttccgccc ttgagctgcg tggcttccaa
     3841 cgggacctga gcagcctgag gcggctggca cagagcttcc ggcccgccat gcggagggtg
     3901 ttcctacatg aggccacggc ccggctgatg gcgggggcca gccccacacg gacacaccag
     3961 ctcctcgacc gcagtctgag gcggcgggca ggccccggtg gcaaaggagg cgcggtggcg
     4021 gagctggagc cgcggcccac gcggcgggag cacgcggagg ccttgctgct ggcctcctgc
     4081 tacctgcccc ccggcttcct gtcggcgccc gggcagcgcg tgggcatgct ggctgaggcg
     4141 gcgcgcacac tcgagaagct tggcgatcgc cggctgctgc acgactgtca gcagatgctc
     4201 atgcgcctgg gcggtgggac cactgtcact tccagcctcg aggaacaaaa actaatatca
     4261 gaggaagatt tggaacagaa actaatcagt gaagaagatt tagagcaaaa gttaatttcc
     4321 gaggaggact tatagtgagg gcccgatatc tcgacaatca acctctggat tacaaaattt
     4381 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4441 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4501 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4561 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4621 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4681 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4741 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     4801 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4861 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4921 tctccctttg ggccgcctcc ccgcctggaa acgggggagg ctaactgaaa cacggaagga
     4981 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     5041 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     5101 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     5161 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     5221 tagcagatct gcgcagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     5281 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5341 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5401 aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5461 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5521 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5581 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg
     5641 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     5701 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5761 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     5821 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     5881 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5941 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     6001 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     6061 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     6121 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     6181 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     6241 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     6301 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     6361 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     6421 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     6481 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     6541 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     6601 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     6661 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     6721 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     6781 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6841 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     6901 ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     6961 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     7021 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     7081 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7141 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     7201 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7261 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7321 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7381 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7441 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7501 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7561 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7621 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7681 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7741 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7801 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     7861 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7921 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7981 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8041 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     8101 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8161 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8221 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8281 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8341 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8401 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8461 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8521 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8581 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8641 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8701 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8761 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8821 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8881 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8941 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9001 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9061 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9121 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9181 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9361 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     9421 cctatggtcg actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     9481 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     9541 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
//
