LOCUS       V032602                13245 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032602
VERSION     V032602
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..7925
                     /label="SPTBN2(NM_006946)"
                     /note="SPTBN2(NM_006946)"
                     /gene="SPTBN2"
     CDS             7932..7997
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    8021..8609
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    8675..8723
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8925..9353
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9367..9696
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             9763..10557
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    10733..10854
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10903..10919)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10927..10943
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10951..10981)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10996..11017
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11305..11893)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12064..12924)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12925..13029)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgag cagcacgctg tcacccacag
      781 actttgacag cttggaaatc cagggccagt acagtgacat caacaaccgc tgggaccttc
      841 ctgactcgga ctgggacaat gacagcagct cggcccgcct ctttgagagg tctcgcatta
      901 aggctctggc agatgaacga gaagctgtgc agaagaaaac cttcaccaag tgggtaaact
      961 cgcacctggc ccgggtcacg tgccgggtgg gggacctgta cagcgacctc cgggacggac
     1021 gcaacctgct gaggctcctc gaggtgctct cgggagagat actgccaaag cctacaaagg
     1081 gccgcatgcg gatccactgc ctggagaacg tggacaaggc actgcagttc ctcaaggagc
     1141 agaaagtgca cttggaaaac atgggctccc atgacattgt ggacggaaac caccgactga
     1201 cccttgggct ggtctggacc atcatccttc gattccagat ccaagacatc agtgtggaga
     1261 cagaagacaa caaggagaag aagtcagcca aggatgccct gcttctgtgg tgccagatga
     1321 agactgcagg ttatcccaac gtcaatgtac acaacttcac caccagctgg agagatggac
     1381 tagctttcaa cgccatcgtg cataaacacc ggccagacct gctggatttt gagtctctga
     1441 agaagtgtaa tgcacactat aatctgcaga atgcattcaa tctggctgaa aaggaactgg
     1501 gacttaccaa gctgctggat cccgaagacg tgaatgtgga ccagccagat gagaagtcaa
     1561 tcattaccta tgtggctact tactaccatt acttctccaa gatgaaggcc ctggccgtgg
     1621 aaggcaagag aattggcaag gtgctggacc atgccatgga ggcagagcgc ctggtggaga
     1681 aatacgagtc cctggcctcg gagctgctgc agtggatcga gcaaacgatc gtgaccctca
     1741 atgaccggca gttggccaac tcccttagcg gggtccagaa ccagctgcag tccttcaact
     1801 cctaccgcac cgtggagaag ccgcccaagt ttaccgagaa agggaacttg gaagtgctgc
     1861 tcttcaccat ccagagcaag cttcgggcca acaaccagaa ggtctacacg ccccgcgagg
     1921 gccggctcat ctcggacatc aacaaggctt gggagcggct ggagaaggcg gagcacgagc
     1981 gtgagctggc cctgcgcacc gagctcatcc gccaggagaa gctggagcag ctggccgccc
     2041 gcttcgaccg caaggctgcc atgcgggaga cctggctcag cgagaaccag cgcctcgtgt
     2101 cccaggacaa ctttgggctg gagctggcag ctgtcgaggc agcagtacgg aagcacgaag
     2161 ccattgagac ggacatcgtg gcctacagcg gccgggtgca ggcagtggac gccgtggctg
     2221 cagagctggc cgccgagcgc taccacgaca tcaagcgcat cgccgctcgg cagcacaacg
     2281 tggcacggct ctgggacttc ttgcggcaga tggtggccgc ccggcgggag cggctcctcc
     2341 tcaacctgga gctgcagaag gtgttccagg acctgctcta cctcatggac tggatggaag
     2401 agatgaaggg ccggctgcag tctcaggacc tgggcaggca cctagcagga gtggaggacc
     2461 tgctgcagct gcacgagctg gtggaggcag acatcgccgt gcaggccgag agggtgcggg
     2521 ccgtcagcgc ctctgccctg cgcttctgca acccagggaa agagtataga ccttgcgacc
     2581 cgcagctggt gtcggagcgg gtggccaagc tagagcagag ctatgaggca ctgtgcgagt
     2641 tggcagcggc gcggcgggcc cggctggagg aatcacggcg gctctggcgt ttcctctggg
     2701 aggtgggtga agctgaggcc tgggtgcggg agcagcagca cctcctggcc tcagccgaca
     2761 cgggccgaga cctgaccggt gccctccgcc tgctcaacaa gcacacagcc ctgcggggcg
     2821 agatgagcgg ccggctgggg cccctgaagc tcaccctgga gcagggccag cagttggtgg
     2881 ccgagggtca ccctggggca agccaggcct ctgcccgtgc agctgaactc caagcccagt
     2941 gggagcggct agaggccctg gccgaggagc gtgcccagcg gctggcccaa gccgccagcc
     3001 tctaccagtt ccaggccgat gcaaacgaca tggaggcctg gttggttgac gcactgcgcc
     3061 tggtgtccag ccccgagctg gggcacgacg agttctccac gcaggctcta gccaggcagc
     3121 atcgggccct ggaggaggag attcgaagcc accggccaac cctggacgcc ttgagggaac
     3181 aggcagcagc cctgcccccc acactgagcc gcacgcccga ggtgcagagc cgggtgccca
     3241 ccctggagcg gcactacgag gagctgcagg cccgggcagg cgagcgagcg cgggccttgg
     3301 aggcagccct ggcgctctac accatgctca gcgaggccgg ggcctgtgga ctctgggtgg
     3361 aggagaagga gcagtggctc aacgggctgg ccctgcctga acgcctggag gacctggagg
     3421 tcgtgcagca gaggttcgag accctggagc ctgaaatgaa cacccttgca gcacaaatca
     3481 ccgcggtgaa tgacattgcc gagcagttac tgaaggccaa ccccccaggc aaagaccgca
     3541 ttgtcaacac ccaggagcag ctcaaccaca ggtggcagca gtttcggcgt ctggcagacg
     3601 gcaagaaggc agctctcacc tcagccctga gcatccagaa ctaccactta gagtgcacgg
     3661 agacccaggc ctggatgaga gagaagacca aagtcatcga gtccacccag ggcctaggca
     3721 acgatctggc tggggtgctg gccctgcagc gcaagctggc cggcacggag cgggacctgg
     3781 aggccatcgc cgcccgggtg ggcgaactga ctcgagaggc aaatgccctg gctgccggcc
     3841 atcccgctca ggcagtggcc atcaacgccc ggctgagaga ggtgcagacc ggctgggagg
     3901 acctcagggc caccatgcgg cgtcgagaag agtcgctggg ggaggcgcgg cggctgcagg
     3961 acttcttgcg cagcttggat gacttccagg cctggctagg ccgcactcag actgctgtgg
     4021 cctctgaaga agggccggcc accctgcctg aggcagaggc cctcctggcc caacatgcag
     4081 ccctgcgggg agaggtggag cgggcccaga gcgagtatag ccggctgcga gccctgggcg
     4141 aggaggtgac ccgggaccag gctgaccccc agtgcctctt cctacgacag cgactggagg
     4201 ccctgggaac tggctgggag gagctgggcc gaatgtggga gagccggcaa ggtcgcctgg
     4261 cccaggccca cggcttccag ggattcctgc gggatgctcg tcaggctgag ggcgtgctca
     4321 gcagccagga atatgttctg tctcacacgg agatgccagg gacactccag gctgctgatg
     4381 ctgccattaa aaaactggag gacttcatga gcaccatgga cgccaatggg gaacggatcc
     4441 acgggctcct ggaggctggc cgccagctgg tatctgaagg caacatccac gccgacaaga
     4501 ttcgggaaaa ggcagactcc attgagagga ggcacaagaa gaatcaagac gcagcgcagc
     4561 aatttctggg ccgtcttcgg gacaaccggg agcagcagca tttcctgcaa gattgtcacg
     4621 agctgaagct ctggatcgac gagaagatgc tgacagccca ggacgtgtcc tatgacgagg
     4681 cccgcaacct gcatactaag tggcagaagc accaggcatt catggccgag ctggctgcca
     4741 acaaagactg gctggacaag gtggacaagg aagggcgaga gctcaccctt gagaagccag
     4801 agctgaaagc cctggtgtcg gagaagctga gagacctgca caggcgctgg gacgagctgg
     4861 agaccaccac ccaagccaag gcccgcagcc tctttgatgc caaccgagct gagctgtttg
     4921 cccagagctg ctgtgccctg gagagctggc tggagagcct gcaggcccag ctgcactcgg
     4981 atgactacgg caaggacctc accagcgtca acatcctgct caagaagcag cagatgctgg
     5041 aatgggagat ggctgtgaga gagaaggagg tggaggcaat ccaggcccag gccaaagcac
     5101 tggcccagga ggaccagggt gcaggggagg tggagagaac ctcgagggcc gtggaggaga
     5161 agttcagggc cttgtgccag cccatgcggg aacgctgccg gcgcctgcag gcttctcgcg
     5221 agcagcacca gttccaccgc gatgtggaag atgagatttt gtgggtgaca gagcggctgc
     5281 ccatggccag ctccatggag catggcaagg acctgcccag cgtccagctt ctcatgaaga
     5341 aaaaccagac cctgcagaaa gagattcagg gccatgagcc ccggatcgcg gacctgaggg
     5401 agcggcagcg tgctctaggt gcagcagcag caggtccaga gctggctgag ctgcaggaaa
     5461 tgtggaaacg cctgggccac gagctggaac ttcgagggaa gcgactggag gatgccctgc
     5521 gagcccagca gttctaccgc gatgccgccg aggcggaggc ctggatgggc gagcaggaat
     5581 tacacatgat gggccaggag aaggccaagg atgagctgag tgcccaggca gaggtgaaga
     5641 agcaccaggt gctggagcaa gccctggccg actacgcgca gaccatccac cagctggcgg
     5701 ccagcagcca ggacatgatt gaccacgagc acccagagag cactcggata tccatccgcc
     5761 aagcccaggt ggacaagctg tatgccggcc tgaaggagct ggctggagag cggcgggagc
     5821 gcctgcagga gcacctccgg ctgtgccagc tccgccgcga gctggatgac ctggaacagt
     5881 ggatccagga gcgcgaggtg gtggcggcct cccacgagct gggccaggac tacgagcatg
     5941 tgactatgct ccgagacaaa ttccgagagt tctcccggga cacaagcacc atcggtcagg
     6001 agcgcgtaga tagcgccaat gcgctggcca atgggctcat tgctgggggc catgctgcac
     6061 gggccaccgt ggccgagtgg aaggacagtc tcaacgaggc ctgggctgac ctgcttgagc
     6121 tgctggacac acggggtcag gtgctggccg cggcgtacga gctgcagcgc ttcctgcacg
     6181 gggcacgcca agccctggcg cgggtgcagc acaagcagca gcagcttccg gacgggactg
     6241 gccgcgacct caacgctgcc gaggccctgc agcgccgaca ctgtgcctac gagcatgaca
     6301 ttcaggccct cagcccccag gtccagcagg tgcaggacga cggccaccgg ctccagaagg
     6361 cctacgctgg agacaaggct gaggagatcg gccgccacat gcaggccgtg gccgaggcct
     6421 gggcccagct tcagggaagc tctgccgccc gccggcagct gctgctggac accacagaca
     6481 agttccgctt cttcaaggct gtccgggaac tgatgctctg gatggatgag gtcaacctgc
     6541 agatggatgc ccaggagcgt ccccgggatg tgtcctccgc ggatctagtc atcaagaacc
     6601 agcaaggcat caaggcagag atagaggccc gggcagaccg cttctcctcc tgcatcgaca
     6661 tggggaagga gctgctggcc aggagccact atgcggccga ggagatctca gagaagctgt
     6721 ctcagctgca ggcacggcgc caggagacag ctgagaagtg gcaggagaag atggactggc
     6781 ttcagctggt tttggaggtg cttgtgtttg gaagagatgc agggatggca gaggcctggc
     6841 tctgcagcca ggagccactg gtgcgcagcg ctgagctggg ttgcacggtc gacgaagttg
     6901 agagcctcat caagcggcac gaggccttcc agaagtcagc agtggcctgg gaggagcgat
     6961 tctgtgcgct ggagaagctt actgcgctag aggagcggga gaaggagcga aagagaaaga
     7021 gggaggagga ggagcggcgg aaacagccgc ctgctcccga acccacagcc agtgtgcctc
     7081 caggggacct ggtgggcggc cagacagctt ctgacaccac ctgggacgga acccagccac
     7141 ggccaccacc atccacacaa gcacccagtg ttaatggagt ctgcacagat ggagagccct
     7201 cacagcccct gctgggacaa cagagacttg agcacagcag cttccccgaa gggccgggac
     7261 ctggctcagg ggacgaagcc aatgggcccc ggggagagag gcagacccgg actcggggcc
     7321 cggccccatc tgcaatgccc cagagcaggt ctaccgagtc agcccatgct gccaccctgc
     7381 cgcctcgagg cccagagcca tctgcccagg agcagatgga ggggatgctg tgccgcaagc
     7441 aggagatgga ggccttcggg aagaaggctg ccaacaggtc ctggcagaac gtgtactgtg
     7501 tcctgcggcg tgggagcctc ggcttttaca aggatgccaa ggcagccagc gcgggagtgc
     7561 cataccacgg agaagtgcct gtcagcctgg ccagggccca gggcagcgtc gcctttgatt
     7621 accgaaagcg caaacatgtc ttcaagctgg gcttacagga tggaaaagaa tatttattcc
     7681 aggccaagga tgaggcagag atgagctcgt ggctacgggt ggtgaatgca gccattgcca
     7741 cagcgtcttc tgcctctgga gagcctgaag agccggtggt gcccagcacc acccggggca
     7801 tgacccgggc catgaccatg cccccagtgt cacccgtcgg ggctgagggg cctgttgtgc
     7861 tccgcagcaa agacggcaga gaacgagagc gagaaaaacg cttcagcttc tttaagaaga
     7921 acaagggatc cgactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca
     7981 aggatgacga tgacaagtag tgagggcccg atatctcgac aatcaacctc tggattacaa
     8041 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     8101 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     8161 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     8221 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     8281 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     8341 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     8401 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     8461 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     8521 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     8581 tcggatctcc ctttgggccg cctccccgcc tggaaacggg ggaggctaac tgaaacacgg
     8641 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     8701 cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     8761 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     8821 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     8881 tgccatagca gatctgcgca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     8941 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     9001 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     9061 ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct
     9121 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     9181 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     9241 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat
     9301 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     9361 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9421 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     9481 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     9541 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     9601 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     9661 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     9721 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     9781 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     9841 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     9901 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     9961 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
    10021 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
    10081 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
    10141 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
    10201 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
    10261 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
    10321 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
    10381 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
    10441 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
    10501 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
    10561 ggactctggg gttcgcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
    10621 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
    10681 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
    10741 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10801 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10861 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
    10921 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    10981 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    11041 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    11101 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    11161 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    11221 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    11281 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    11341 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11401 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11461 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
    11521 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    11581 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    11641 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11701 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
    11761 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11821 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    11881 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    11941 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    12001 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    12061 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    12121 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    12181 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    12241 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    12301 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    12361 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    12421 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    12481 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    12541 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    12601 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    12661 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    12721 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    12781 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    12841 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    12901 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    12961 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    13021 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
    13081 gatcccctat ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
    13141 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
    13201 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagg
//
