LOCUS       V041402                 9409 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041402
VERSION     V041402
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..4020
                     /label="SMARCC1(NM_003074)"
                     /note="SMARCC1(NM_003074)"
                     /gene="SMARCC1"
     CDS             4021..4068
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /transl_table=1
                     /translation="KRPAATKKAGQAKKKK"
                     /label="nucleoplasmin NLS"
     CDS             4075..4101
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4105..4131
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4135..4161
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    4185..4773
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4839..4887
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5089..5517
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5531..5860
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5927..6721
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6897..7018
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7067..7083)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7091..7107
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7115..7145)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7160..7181
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7469..8057)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8228..9088)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9089..9193)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc cgcagcggcg
      721 ggcggcggcg ggccggggac agcggtaggc gccacgggct cggggattgc ggcggcagcc
      781 gcaggcctag ctgtttatcg acggaaggat gggggcccgg ccaccaagtt ttgggagagc
      841 ccggagacgg tgtcccagct ggattcggtg cgggtctggc tgggcaagca ctacaagaag
      901 tatgttcatg cggatgctcc taccaataaa acactggctg ggctggtggt gcagcttctt
      961 cagttccagg aagatgcctt tgggaagcat gtcaccaacc cggccttcac caaactccct
     1021 gcaaagtgtt tcatggattt caaagctgga ggcgccttat gtcacattct tggggctgct
     1081 tacaagtata aaaatgaaca gggatggcgg aggtttgacc tacagaaccc atctcgaatg
     1141 gatcgtaatg tggaaatgtt tatgaacatt gaaaaaacat tggtgcagaa caattgtttg
     1201 accagaccca acatctacct cattccagac attgatctga agttggctaa caaattgaaa
     1261 gatatcatca aacgacatca gggaacattt acggatgaga agtcaaaagc ttcccaccac
     1321 atttacccat attcttcctc acaagacgat gaagaatggt tgagaccggt gatgagaaaa
     1381 gagaagcaag tgttagtgca ttggggcttt tacccagaca gctatgatac ttgggtccat
     1441 agtaatgatg ttgatgctga aattgaagat ccaccaattc cagaaaaacc atggaaggtt
     1501 catgtgaaat ggattttgga cactgatatt ttcaatgaat ggatgaatga ggaggattat
     1561 gaggtggatg aaaataggaa gcctgtgagt tttcgtcagc ggatttcaac caagaatgaa
     1621 gagccagtca gaagtccaga aagaagagat agaaaagcat cagctaatgc tcgaaagagg
     1681 aaacattcgc cttcgcctcc ccctccgaca ccaacagaat cacggaagaa gagtgggaag
     1741 aaaggccaag ctagccttta tgggaagcgc agaagtcaga aagaggaaga tgagcaagaa
     1801 gatctaacca aggatatgga agacccaaca cctgtaccca atatagaaga agtagtactt
     1861 cccaaaaatg tgaacctaaa gaaagatagt gaaaatacac ctgttaaagg aggaactgta
     1921 gcggatctag atgagcagga tgaagaaaca gtcacagcag gaggaaagga agatgaagat
     1981 cctgccaaag gtgatcagag tcgatcagtt gaccttgggg aagataatgt gacagagcag
     2041 accaatcaca ttattattcc tagttatgca tcatggtttg attataactg tattcatgtg
     2101 attgaacggc gtgctcttcc tgagttcttc aatggaaaaa acaaatccaa gactccagaa
     2161 atatacttgg catatcgaaa ttttatgatt gacacgtatc gtctaaaccc ccaagagtat
     2221 ttaactagca ctgcttgtcg gaggaacttg actggagatg tgtgtgctgt gatgagggtc
     2281 catgcctttt tagagcagtg gggactcgtt aattaccaag ttgacccgga aagtagaccc
     2341 atggcaatgg gacctcctcc tactcctcat tttaatgtat tagctgatac cccctctggg
     2401 cttgtgcctc tgcatcttcg atcacctcag gttcctgctg ctcaacagat gctaaatttt
     2461 cctgagaaaa acaaggaaaa accagttgat ttgcagaact ttggtctccg tactgacatt
     2521 tactccaaga aaacattagc aaagagtaaa ggtgctagtg ctggaagaga atggactgaa
     2581 caggagaccc ttctactcct ggaggccctg gagatgtaca aggatgattg gaacaaagtg
     2641 tcggaacatg ttggaagtcg tactcaggat gaatgcatcc tccacttttt gagacttccc
     2701 attgaggacc cataccttga gaattcagat gcttcccttg ggcctttggc ctaccagcct
     2761 gtccccttca gtcagtcagg aaatccagtt atgagtactg ttgctttttt ggcatctgtg
     2821 gtggaccctc gcgtggcatc tgctgcagca aaagcggctt tggaggagtt ttctcgggtc
     2881 cgggaggagg taccactgga attggttgaa gctcatgtca agaaagtaca agaagcagca
     2941 cgagcctctg ggaaagtgga tcccacctac ggtctggaga gcagctgcat tgcaggcaca
     3001 gggcccgatg agccagagaa gcttgaagga gctgaagagg aaaaaatgga agccgaccct
     3061 gatggtcagc agcctgaaaa ggcagaaaat aaagtggaaa atgaaacgga tgaaggtgat
     3121 aaagcacaag atggagaaaa tgaaaaaaat agtgaaaagg aacaggatag tgaagtgagt
     3181 gaggatacca aatcagaaga aaaggagact gaagagaaca aagaactcac tgatacatgt
     3241 aaagaaagag aaagtgatac tgggaagaag aaagtagaac atgaaatttc cgaaggaaat
     3301 gttgccacag ccgcagcagc tgctcttgcc tcagcggcta ccaaagccaa gcacctggct
     3361 gcagtggaag aaagaaagat caagtccctg gtagctctct tggttgagac acaaatgaag
     3421 aaactagaga tcaaacttcg acattttgaa gagctggaaa ctatcatgga cagagagaaa
     3481 gaagctctag aacaacagag gcagcagttg cttactgaac gccaaaactt ccacatggaa
     3541 cagctgaagt atgctgaatt acgagcacga cagcaaatgg aacagcagca gcatggccag
     3601 aaccctcaac aggcacacca gcactcagga ggacctggcc tggccccact tggagcagca
     3661 gggcaccctg gcatgatgcc tcatcaacag ccccctccct accctctgat gcaccaccag
     3721 atgccaccac ctcatccacc ccagccaggt cagataccag gcccaggttc catgatgccc
     3781 gggcagcaca tgccaggccg catgattccc actgttgcag ccaacatcca cccctctggg
     3841 agtggcccta cccctcctgg catgccacca atgccaggaa acatcttagg accccgggta
     3901 cccctgacag cacctaacgg catgtatccc cctccaccac agcagcagcc accgccacca
     3961 ccacctgcag atggggtccc tccgcctcct gctcctggcc cgccagcctc agctgctcct
     4021 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaagct cgagtaccca
     4081 tacgacgtac ctgattatgc aacttatcct tatgatgtac ctgattatgc tagctatcct
     4141 tatgatgtcc cagactacgc atagtgaggg cccgatatct cgacaatcaa cctctggatt
     4201 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4261 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4321 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4381 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4441 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4501 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4561 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     4621 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4681 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4741 cgagtcggat ctccctttgg gccgcctccc cgcctggaaa cgggggaggc taactgaaac
     4801 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     4861 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     4921 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     4981 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     5041 gccctgccat agcagatctg cgcagctggg gctctagggg gtatccccac gcgccctgta
     5101 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5161 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5221 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc
     5281 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5341 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5401 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg
     5461 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     5521 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     5581 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     5641 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     5701 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5761 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     5821 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     5881 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     5941 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     6001 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     6061 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     6121 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     6181 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     6241 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     6301 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     6361 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     6421 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     6481 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     6541 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     6601 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     6661 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     6721 agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     6781 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6841 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6901 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6961 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7021 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     7081 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7141 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7201 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7261 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7321 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7381 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7441 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7501 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7561 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7621 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     7681 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7741 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7801 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7861 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7921 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7981 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8041 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8101 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8161 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8221 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8281 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8341 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8401 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8461 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8521 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8581 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8641 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8701 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8761 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8821 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8881 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8941 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9001 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9061 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9121 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9181 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     9241 tcccgatccc ctatggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca
     9301 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     9361 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttagg
//
