LOCUS       V043608                 9315 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043608
VERSION     V043608
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..4041
                     /label="SENP6(NM_015571)"
                     /note="SENP6(NM_015571)"
                     /gene="SENP6"
     CDS             4048..4071
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHHHH"
                     /label="8xHis"
     misc_feature    4091..4679
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4745..4793
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4995..5423
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5437..5766
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5833..6627
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6803..6924
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6973..6989)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6997..7013
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7021..7051)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7066..7087
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7375..7963)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8134..8994)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8995..9099)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc ggccggcaag
      721 agcggcggta gcgcagggga gattactttt ctggaagctt tggctagatc agagtctaag
      781 agagatggag gttttaaaaa taattggagc tttgatcatg aagaagaaag tgaaggagat
      841 acagataaag atgggacaaa tctgctcagt gtggatgaag atgaggattc tgaaacctca
      901 aaaggaaaaa agttaaatcg tcgatctgaa attgttgcta atagctctgg tgaattcatc
      961 ttgaagacat atgtaagacg aaacaagtct gaaagtttta aaactttgaa aggcaaccca
     1021 attggactta acatgttgag caacaataag aaattgagtg aaaatacgca aaatacgtca
     1081 ttatgttctg gaactgtagt tcatggtaga cgttttcatc atgctcatgc acagatacca
     1141 gtagtaaaaa cagcagccca aagcagtctg gaccgaaaag aaaggaaaga atacccacct
     1201 catgtccaaa aagttgaaat taatcctgta aggttaagtc ggctccaagg tgttgaacgt
     1261 ataatgaaga aaacagaaga gtccgaatca caagtggagc ctgaaattaa gaggaaagta
     1321 caacagaaac gacactgtag tacctatcag cctactcctc ctctatctcc tgcttcaaaa
     1381 aaatgtttaa cccatttaga ggatttgcaa agaaattgca gacaagctat tactttgaat
     1441 gagtctactg gaccattatt aagaacgtca attcatcaga attctggagg acagaagtca
     1501 caaaacacag gattaacaac caagaagttt tatggcaaca atgtggaaaa ggttccaatt
     1561 gatattattg tgaattgtga tgacagtaaa cacacttatt tacagactaa tggaaaagtc
     1621 attttacctg gggcaaaaat acccaaaatc acaaacttga aagaaaggaa aacaagtttg
     1681 tcagacctaa atgatccaat cattttgtcc agtgatgatg atgatgacaa cgacagaact
     1741 aacagaagag aaagcatatc tcctcagcct gctgattcag catgttcttc ccctgcacca
     1801 tccactggaa aagtagaagc agcgctaaat gaaaatactt gcagagcaga gcgtgaacta
     1861 cgaagcattc cagaagactc agagttaaat acagttacat tgccaagaaa agcaagaatg
     1921 aaagaccagt ttggcaattc tattatcaac acacctctga aacgtcgtaa agtgttttct
     1981 caagaacctc cagatgcttt agctttaagc tgccaaagtt cctttgacag tgtcatttta
     2041 aactgtcgaa gtatacgagt aggaacactc ttccggctgt taatagagcc tgtaattttt
     2101 tgtttagatt ttatcaagat acagctagac gaaccagacc atgatcctgt agagattata
     2161 ttaaatacct ctgatctaac taaatgtgaa tggtgtaatg tccgaaaatt acctgtagtg
     2221 tttcttcaag caattccagc agtttatcaa aagctgagca tccaactgca aatgaataag
     2281 gaggataaag tttggaatga ttgtaaagga gtaaataaat taacaaattt agaagaacaa
     2341 tatataattt taatttttca aaatggcctt gatcctccgg caaatatggt atttgaaagt
     2401 atcattaatg aaattggtat aaagaataac atctccaatt tttttgcgaa aattcccttt
     2461 gaagaagcta atggcagact tgttgcctgt acaagaacct atgaagagag catcaaagga
     2521 agttgtgggc aaaaggaaaa caaaattaaa actgtatcat ttgaatctaa aatacaactt
     2581 agaagcaaac aagaatttca gttttttgat gaagaagaag aaactggaga aaaccacacc
     2641 atcttcattg gcccagtaga aaagttgata gtatatccac cacctccagc taagggaggc
     2701 atctctgtta ccaatgagga cctgcactgt ctaaatgaag gagaattttt aaatgatgtt
     2761 attatagact tttatttgaa atacttggtg cttgaaaaac tgaagaagga agacgctgac
     2821 cgaattcata tattcagttc ttttttctat aaacgcctta atcagagaga gaggagaaat
     2881 catgaaacaa ctaatctgtc aatacagcaa aaacggcatg ggagagtaaa aacatggacc
     2941 cggcacgtag atatttttga gaaggatttt atttttgtac cccttaatga agctgcacac
     3001 tggtttttgg ctgttgtttg tttccccggt ttggaaaaac caaagtatga acctaatcct
     3061 cattaccatg aaaatgctgt catacagaaa tgttcaactg tagaggacag ttgtatttct
     3121 tcttcagcca gtgaaatgga gagttgttca caaaactctt ctgccaagcc tgtaattaag
     3181 aagatgctaa acaaaaaaca ttgcatagct gtaattgatt ccaatcctgg gcaggaagaa
     3241 agtgaccctc gttataagag aaacatatgc agtgtaaaat acagtgtgaa aaaaataaat
     3301 catactgcga gtgaaaatga agaattcaat aaaggagaat ctacatccca gaaagttgct
     3361 gataggacta aaagtgagaa tggcctacag aatgaaagtt taagttccac acatcataca
     3421 gatggcttaa gcaaaatcag actaaactat agcgatgaat cacctgaagc tggtaaaatg
     3481 cttgaagatg aactcgtcga cttctcagaa gatcaggata accaggatga tagcagtgac
     3541 gatggattcc tcgctgatga caactgcagt tcagaaatag gacagtggca tttaaagcct
     3601 actatctgta aacaaccttg tatcctactt atggactcac tccgaggccc ttctcggtca
     3661 aatgttgtca aaattttaag agagtattta gaagtggaat gggaagttaa aaaaggaagc
     3721 aaaagaagtt tttccaaaga tgttatgaag ggctctaatc caaaagtacc acagcaaaac
     3781 aacttcagtg actgtggtgt atatgtattg cagtatgtag agagcttttt tgagaatcca
     3841 attctcagtt ttgaactacc tatgaatttg gcaaactggt ttcctccacc aagaatgaga
     3901 acaaaaagag aagaaatccg aaacataatt ctgaagctac aggaagatca gagcaaagag
     3961 aaaagaaagc ataaggacac ttactcaaca gaagcacctt taggcgaagg aacagaacaa
     4021 tatgtcaata gtatctcaga tctcgagcac catcaccatc accatcacca ttagggcccg
     4081 atatctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4141 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4201 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4261 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4321 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4381 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4441 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     4501 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4561 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4621 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4681 tggaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     4741 atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg
     4801 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     4861 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     4921 gctcgcagcc aacgtcgggg cggcaggccc tgccatagca gatctgcgca gctggggctc
     4981 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5041 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5101 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt
     5161 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     5221 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5281 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     5341 ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat
     5401 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     5461 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5521 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5581 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5641 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5701 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5761 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     5821 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     5881 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     5941 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     6001 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     6061 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     6121 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     6181 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     6241 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     6301 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     6361 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     6421 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     6481 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     6541 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     6601 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca
     6661 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     6721 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     6781 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     6841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6901 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     6961 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7021 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7081 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7141 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7561 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7801 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9121 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc
     9181 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     9241 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     9301 aagaatctgc ttagg
//
