LOCUS V021571 12029 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021571 VERSION V021571 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 683..6709 /label="SCN1A(NM_001165963)" /note="SCN1A(NM_001165963)" /gene="SCN1A" CDS 6716..6781 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 6805..7393 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 7459..7507 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 7709..8137 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 8151..8480 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 8547..9341 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 9517..9638 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(9687..9703) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 9711..9727 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(9735..9765) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 9780..9801 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(10089..10677) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10848..11708) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(11709..11813) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccgcca ccatggagca aacagtgctt gtaccaccag gacctgacag 721 cttcaacttc ttcaccagag aatctcttgc ggctattgaa agacgcattg cagaagaaaa 781 ggcaaagaat cccaaaccag acaaaaaaga tgacgacgaa aatggcccaa agccaaatag 841 tgacttggaa gctggaaaga accttccatt tatttatgga gacattcctc cagagatggt 901 gtcagagccc ctggaggacc tggaccccta ctatatcaat aagaaaactt ttatagtatt 961 gaataaaggg aaggccatct tccggttcag tgccacctct gccctgtaca ttttaactcc 1021 cttcaatcct cttaggaaaa tagctattaa gattttggta cattcattat tcagcatgct 1081 aattatgtgc actattttga caaactgtgt gtttatgaca atgagtaacc ctcctgattg 1141 gacaaagaat gtagaataca ccttcacagg aatatatact tttgaatcac ttataaaaat 1201 tattgcaagg ggattctgtt tagaagattt tactttcctt cgggatccat ggaactggct 1261 cgatttcact gtcattacat ttgcgtacgt cacagagttt gtggacctgg gcaatgtctc 1321 ggcattgaga acattcagag ttctccgagc attgaagacg atttcagtca ttccaggcct 1381 gaaaaccatt gtgggagccc tgatccagtc tgtgaagaag ctctcagatg taatgatcct 1441 gactgtgttc tgtctgagcg tatttgctct aattgggctg cagctgttca tgggcaacct 1501 gaggaataaa tgtatacaat ggcctcccac caatgcttcc ttggaggaac atagtataga 1561 aaagaatata actgtgaatt ataatggtac acttataaat gaaactgtct ttgagtttga 1621 ctggaagtca tatattcaag attcaagata tcattatttc ctggagggtt ttttagatgc 1681 actactatgt ggaaatagct ctgatgcagg ccaatgtcca gagggatata tgtgtgtgaa 1741 agctggtaga aatcccaatt atggctacac aagctttgat accttcagtt gggctttttt 1801 gtccttgttt cgactaatga ctcaggactt ctgggaaaat ctttatcaac tgacattacg 1861 tgctgctggg aaaacgtaca tgatattttt tgtattggtc attttcttgg gctcattcta 1921 cctaataaat ttgatcctgg ctgtggtggc catggcctac gaggaacaga atcaggccac 1981 cttggaagaa gcagaacaga aagaggccga atttcagcag atgattgaac agcttaaaaa 2041 gcaacaggag gcagctcagc aggcagcaac ggcaactgcc tcagaacatt ccagagagcc 2101 cagtgcagca ggcaggctct cagacagctc atctgaagcc tctaagttga gttccaagag 2161 tgctaaggaa agaagaaatc ggaggaagaa aagaaaacag aaagagcagt ctggtgggga 2221 agagaaagat gaggatgaat tccaaaaatc tgaatctgag gacagcatca ggaggaaagg 2281 ttttcgcttc tccattgaag ggaaccgatt gacatatgaa aagaggtact cctccccaca 2341 ccagtctttg ttgagcatcc gtggctccct attttcacca aggcgaaata gcagaacaag 2401 ccttttcagc tttagagggc gagcaaagga tgtgggatct gagaacgact tcgcagatga 2461 tgagcacagc acctttgagg ataacgagag ccgtagagat tccttgtttg tgccccgacg 2521 acacggagag agacgcaaca gcaacctgag tcagaccagt aggtcatccc ggatgctggc 2581 agtgtttcca gcgaatggga agatgcacag cactgtggat tgcaatggtg tggtttcctt 2641 ggttggtgga ccttcagttc ctacatcgcc tgttggacag cttctgccag aggtgataat 2701 agataagcca gctactgatg acaatggaac aaccactgaa actgaaatga gaaagagaag 2761 gtcaagttct ttccacgttt ccatggactt tctagaagat ccttcccaaa ggcaacgagc 2821 aatgagtata gccagcattc taacaaatac agtagaagaa cttgaagaat ccaggcagaa 2881 atgcccaccc tgttggtata aattttccaa catattctta atctgggact gttctccata 2941 ttggttaaaa gtgaaacatg ttgtcaacct ggttgtgatg gacccatttg ttgacctggc 3001 catcaccatc tgtattgtct taaatactct tttcatggcc atggagcact atccaatgac 3061 ggaccatttc aataatgtgc ttacagtagg aaacttggtt ttcactggga tctttacagc 3121 agaaatgttt ctgaaaatta ttgccatgga tccttactat tatttccaag aaggctggaa 3181 tatctttgac ggttttattg tgacgcttag cctggtagaa cttggactcg ccaatgtgga 3241 aggattatct gttctccgtt catttcgatt gctgcgagtt ttcaagttgg caaaatcttg 3301 gccaacgtta aatatgctaa taaagatcat cggcaattcc gtgggggctc tgggaaattt 3361 aaccctcgtc ttggccatca tcgtcttcat ttttgccgtg gtcggcatgc agctctttgg 3421 taaaagctac aaagattgtg tctgcaagat cgccagtgat tgtcaactcc cacgctggca 3481 catgaatgac ttcttccact ccttcctgat tgtgttccgc gtgctgtgtg gggagtggat 3541 agagaccatg tgggactgta tggaggttgc tggtcaagcc atgtgcctta ctgtcttcat 3601 gatggtcatg gtgattggaa acctagtggt cctgaatctc tttctggcct tgcttctgag 3661 ctcatttagt gcagacaacc ttgcagccac tgatgatgat aatgaaatga ataatctcca 3721 aattgctgtg gataggatgc acaaaggagt agcttatgtg aaaagaaaaa tatatgaatt 3781 tattcaacag tccttcatta ggaaacaaaa gattttagat gaaattaaac cacttgatga 3841 tctaaacaac aagaaagaca gttgtatgtc caatcataca gcagaaattg ggaaagatct 3901 tgactatctt aaagatgtaa atggaactac aagtggtata ggaactggca gcagtgttga 3961 aaaatacatt attgatgaaa gtgattacat gtcattcata aacaacccca gtcttactgt 4021 gactgtacca attgctgtag gagaatctga ctttgaaaat ttaaacacgg aagactttag 4081 tagtgaatcg gatctggaag aaagcaaaga gaaactgaat gaaagcagta gctcatcaga 4141 aggtagcact gtggacatcg gcgcacctgt agaagaacag cccgtagtgg aacctgaaga 4201 aactcttgaa ccagaagctt gtttcactga aggctgtgta caaagattca agtgttgtca 4261 aatcaatgtg gaagaaggca gaggaaaaca atggtggaac ctgagaagga cgtgtttccg 4321 aatagttgaa cataactggt ttgagacctt cattgttttc atgattctcc ttagtagtgg 4381 tgctctggca tttgaagata tatatattga tcagcgaaag acgattaaga cgatgttgga 4441 atatgctgac aaggttttca cttacatttt cattctggaa atgcttctaa aatgggtggc 4501 atatggctat caaacatatt tcaccaatgc ctggtgttgg ctggacttct taattgttga 4561 tgtttcattg gtcagtttaa cagcaaatgc cttgggttac tcagaacttg gagccatcaa 4621 atctctcagg acactaagag ctctgagacc tctaagagcc ttatctcgat ttgaagggat 4681 gagggtggtt gtgaatgccc ttttaggagc aattccatcc atcatgaatg tgcttctggt 4741 ttgtcttata ttctggctaa ttttcagcat catgggcgta aatttgtttg ctggcaaatt 4801 ctaccactgt attaacacca caactggtga caggtttgac atcgaagacg tgaataatca 4861 tactgattgc ctaaaactaa tagaaagaaa tgagactgct cgatggaaaa atgtgaaagt 4921 aaactttgat aatgtaggat ttgggtatct ctctttgctt caagttgcca cattcaaagg 4981 atggatggat ataatgtatg cagcagttga ttccagaaat gtggaactcc agcctaagta 5041 tgaagaaagt ctgtacatgt atctttactt tgttattttc atcatctttg ggtccttctt 5101 caccttgaac ctgtttattg gtgtcatcat agataatttc aaccagcaga aaaagaagtt 5161 tggaggtcaa gacatcttta tgacagaaga acagaagaaa tactataatg caatgaaaaa 5221 attaggatcg aaaaaaccgc aaaagcctat acctcgacca ggaaacaaat ttcaaggaat 5281 ggtctttgac ttcgtaacca gacaagtttt tgacataagc atcatgattc tcatctgtct 5341 taacatggtc acaatgatgg tggaaacaga tgaccagagt gaatatgtga ctaccatttt 5401 gtcacgcatc aatctggtgt tcattgtgct atttactgga gagtgtgtac tgaaactcat 5461 ctctctacgc cattattatt ttaccattgg atggaatatt tttgattttg tggttgtcat 5521 tctctccatt gtaggtatgt ttcttgccga gctgatagaa aagtatttcg tgtcccctac 5581 cctgttccga gtgatccgtc ttgctaggat tggccgaatc ctacgtctga tcaaaggagc 5641 aaaggggatc cgcacgctgc tctttgcttt gatgatgtcc cttcctgcgt tgtttaacat 5701 cggcctccta ctcttcctag tcatgttcat ctacgccatc tttgggatgt ccaactttgc 5761 ctatgttaag agggaagttg ggatcgatga catgttcaac tttgagacct ttggcaacag 5821 catgatctgc ctattccaaa ttacaacctc tgctggctgg gatggattgc tagcacccat 5881 tctcaacagt aagccacccg actgtgaccc taataaagtt aaccctggaa gctcagttaa 5941 gggagactgt gggaacccat ctgttggaat tttctttttt gtcagttaca tcatcatatc 6001 cttcctggtt gtggtgaaca tgtacatcgc ggtcatcctg gagaacttca gtgttgctac 6061 tgaagaaagt gcagagcctc tgagtgagga tgactttgag atgttctatg aggtttggga 6121 gaagtttgat cccgatgcaa ctcagttcat ggaatttgaa aaattatctc agtttgcagc 6181 tgcgcttgaa ccgcctctca atctgccaca accaaacaaa ctccagctca ttgccatgga 6241 tttgcccatg gtgagtggtg accggatcca ctgtcttgat atcttatttg cttttacaaa 6301 gcgggttcta ggagagagtg gagagatgga tgctctacga atacagatgg aagagcgatt 6361 catggcttcc aatccttcca aggtctccta tcagccaatc actactactt taaaacgaaa 6421 acaagaggaa gtatctgctg tcattattca gcgtgcttac agacgccacc ttttaaagcg 6481 aactgtaaaa caagcttcct ttacgtacaa taaaaacaaa atcaaaggtg gggctaatct 6541 tcttataaaa gaagacatga taattgacag aataaatgaa aactctatta cagaaaaaac 6601 tgatctgacc atgtccactg cagcttgtcc accttcctat gaccgggtga caaagccaat 6661 tgtggaaaaa catgagcaag aaggcaaaga tgaaaaagcc aaagggaaac tcgaggacta 6721 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa 6781 gtagtgaggg cccgatatct cgacaatcaa cctctggatt acaaaatttg tgaaagattg 6841 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 6901 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 6961 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 7021 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 7081 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 7141 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 7201 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 7261 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 7321 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 7381 gccgcctccc cgcctggaaa cgggggaggc taactgaaac acggaaggag acaataccgg 7441 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg 7501 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga 7561 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg 7621 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcagatctg 7681 cgcagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg 7741 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 7801 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 7861 cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 7921 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 7981 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 8041 cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta 8101 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 8161 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 8221 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 8281 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 8341 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 8401 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg 8461 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc 8521 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 8581 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 8641 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 8701 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca 8761 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 8821 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 8881 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 8941 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 9001 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 9061 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 9121 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 9181 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 9241 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 9301 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc 9361 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc 9421 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 9481 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat 9541 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 9601 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc 9661 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 9721 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 9781 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 9841 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 9901 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 9961 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 10021 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 10081 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 10141 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 10201 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 10261 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc 10321 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 10381 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 10441 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 10501 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 10561 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 10621 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 10681 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 10741 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 10801 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 10861 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 10921 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 10981 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 11041 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 11101 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 11161 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 11221 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 11281 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 11341 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 11401 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 11461 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 11521 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 11581 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 11641 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 11701 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 11761 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 11821 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtcga 11881 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg 11941 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga 12001 ccgacaattg catgaagaat ctgcttagg //