LOCUS V031464 5516 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031464 VERSION V031464 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1004..1714 /label="Rexo2(NM_024233)" /note="Rexo2(NM_024233)" /gene="Rexo2" CDS 1727..1756 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" primer_bind complement(1773..1789) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 1800..2422 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2571..3159) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3488..3535 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(3767..4561) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4596..4953) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(4955..5059) /gene="bla" /label="AmpR promoter" rep_origin 5086..5514 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccgcc accatgctag gcgtatccct 1021 gggcgccagg ctgttgcggg gcgtgggtgg caggcgcggg cagttcgggg cgcgaggtgt 1081 cagcgaaggt agcgcagcca tggcggcagg ggagagcatg gctcagcgaa tggtctgggt 1141 ggacctggag atgacaggat tggatattga gaaggatcag attattgaga tggcctgcct 1201 gataactgac tctgatctta acattttggc tgaaggtccc aacctgatta tcaaacagcc 1261 ggacgagttg ctggacagca tgtcagactg gtgcaaggaa catcacggga agtctggtct 1321 taccaaggca gtgaaggaga gcacagtcac actgcagcag gcagagtatg aatttctgtc 1381 ctttgtacga cagcagactc ctccggggct ctgtccactt gcagggaact cagttcatgc 1441 agataagaag tttcttgaca aacacatgcc ccagttcatg aaacatcttc actataggat 1501 aattgatgtg agcactgtta aagagctgtg cagacgctgg tatccagaag attatgaatt 1561 tgcaccgaag aaggctgctt ctcacagggc actggatgac attagtgaaa gcatcaaaga 1621 gcttcagttt taccgaaata acatcttcaa gaaaaagaca gacgaaaaga agaggaaaat 1681 tatagaaaat ggagaaactg agaagcctgt gagtacgcgg ccgctcgagc agaaactcat 1741 ctcagaagag gatctgtaaa cggccggccg cggtcatagc tgtttcctga acagatcccg 1801 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag 1861 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct 1921 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa 1981 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc 2041 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt 2101 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg 2161 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt 2221 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga 2281 ttttaaaata actataccag caggaggacg tccagacaca gcataggcta cctggccatg 2341 cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc gttgggtcca 2401 ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc agctcactca 2461 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 2521 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 2581 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 2641 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 2701 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 2761 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 2821 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 2881 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 2941 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 3001 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 3061 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 3121 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 3181 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 3241 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 3301 agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt ggctgcgagc 3361 cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat 3421 tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg 3481 cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat tgccgtcata 3541 gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc cctagggtgg 3601 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa 3661 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc 3721 aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt 3781 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga 3841 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta 3901 tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc 3961 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc 4021 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct 4081 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga 4141 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc 4201 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat 4261 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga 4321 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt 4381 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg 4441 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc 4501 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca 4561 tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag gcctccaaaa 4621 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa 4681 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc 4741 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc 4801 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga 4861 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga 4921 cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa tattattgaa 4981 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 5041 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg 5101 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 5161 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 5221 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 5281 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 5341 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 5401 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 5461 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aatttt //