LOCUS       V031464                 5516 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031464
VERSION     V031464
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1004..1714
                     /label="Rexo2(NM_024233)"
                     /note="Rexo2(NM_024233)"
                     /gene="Rexo2"
     CDS             1727..1756
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     primer_bind     complement(1773..1789)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1800..2422
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2571..3159)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3488..3535
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3767..4561)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4596..4953)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4955..5059)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5086..5514
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccgcc accatgctag gcgtatccct
     1021 gggcgccagg ctgttgcggg gcgtgggtgg caggcgcggg cagttcgggg cgcgaggtgt
     1081 cagcgaaggt agcgcagcca tggcggcagg ggagagcatg gctcagcgaa tggtctgggt
     1141 ggacctggag atgacaggat tggatattga gaaggatcag attattgaga tggcctgcct
     1201 gataactgac tctgatctta acattttggc tgaaggtccc aacctgatta tcaaacagcc
     1261 ggacgagttg ctggacagca tgtcagactg gtgcaaggaa catcacggga agtctggtct
     1321 taccaaggca gtgaaggaga gcacagtcac actgcagcag gcagagtatg aatttctgtc
     1381 ctttgtacga cagcagactc ctccggggct ctgtccactt gcagggaact cagttcatgc
     1441 agataagaag tttcttgaca aacacatgcc ccagttcatg aaacatcttc actataggat
     1501 aattgatgtg agcactgtta aagagctgtg cagacgctgg tatccagaag attatgaatt
     1561 tgcaccgaag aaggctgctt ctcacagggc actggatgac attagtgaaa gcatcaaaga
     1621 gcttcagttt taccgaaata acatcttcaa gaaaaagaca gacgaaaaga agaggaaaat
     1681 tatagaaaat ggagaaactg agaagcctgt gagtacgcgg ccgctcgagc agaaactcat
     1741 ctcagaagag gatctgtaaa cggccggccg cggtcatagc tgtttcctga acagatcccg
     1801 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
     1861 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
     1921 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
     1981 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
     2041 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
     2101 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
     2161 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
     2221 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
     2281 ttttaaaata actataccag caggaggacg tccagacaca gcataggcta cctggccatg
     2341 cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc gttgggtcca
     2401 ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc agctcactca
     2461 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2521 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2581 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2641 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2701 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2761 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2821 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2881 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2941 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3001 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3061 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3121 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3181 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3241 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3301 agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt ggctgcgagc
     3361 cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat
     3421 tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg
     3481 cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat tgccgtcata
     3541 gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc cctagggtgg
     3601 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa
     3661 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc
     3721 aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt
     3781 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     3841 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     3901 tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     3961 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     4021 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     4081 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     4141 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     4201 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     4261 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     4321 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     4381 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     4441 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     4501 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     4561 tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag gcctccaaaa
     4621 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa
     4681 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     4741 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     4801 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     4861 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     4921 cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa tattattgaa
     4981 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5041 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
     5101 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5161 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5221 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5281 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5341 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5401 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5461 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aatttt
//
