LOCUS       V029865                 5562 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029865
VERSION     V029865
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1709
                     /label="Rab3c(NM_023852)"
                     /note="Rab3c(NM_023852)"
                     /gene="Rab3c"
     CDS             1728..1757
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1776..1799
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1819..1835)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1846..2468
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2617..3205)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3534..3581
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3813..4607)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4642..4999)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5001..5105)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5132..5560
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gagacacgag gcgcccatgc agatggcctc tgcacaagat gccaggtttg
     1081 gtcagaaaga ctcttctgac cagaacttcg actacatgtt caagttgctg atcattggca
     1141 atagcagcgt gggcaaaaca tctttcctgt tccgttatgc cgacgattcc tttacatctg
     1201 cattcgtcag cacggttggc atcgatttca aagtaaaaac tgtattcaaa aatgagaaga
     1261 gaatcaagct tcagatttgg gacacagcag gccaggaaag atacaggacc atcaccacag
     1321 cctactatcg aggggccatg ggcttcattt taatgtatga catcacaaat gaagaatcct
     1381 tcaacgctgt acaagattgg tcaactcaga tcaaaacata ctcttgggat aatgcccagg
     1441 ttatcctggc tggaaacaaa tgtgacatgg aagatgagcg ggttgtctca accgagagag
     1501 gccagcgctt aggagaacag ctcgggtttg agttttttga aaccagcgcc aaggataaca
     1561 tcaacgtgaa gcaaaccttc gagcgcctcg tagatatcat ttgtgacaaa atgtcagaga
     1621 gcttggagac cgacccagcc atcacagcgg caaagcagag cacgaggctc aaggagaccc
     1681 ctcctccacc acagcccaac tgtggctgca cgcgtacgcg gccgctcgag cagaaactca
     1741 tctcagaaga ggatctggca gcaaatgata tcctggatta caaggatgac gacgataagg
     1801 tttaaacggc cggccgcggt catagctgtt tcctgaacag atcccgggtg gcatccctgt
     1861 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     1921 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     1981 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     2041 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     2101 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     2161 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     2221 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     2281 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta
     2341 taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga
     2401 catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct
     2461 gttgaattgg gtacgcggcc agcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2521 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2581 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2641 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2701 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2761 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2821 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2881 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2941 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3001 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3061 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3121 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3181 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3241 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3301 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3361 acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc
     3421 cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg
     3481 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
     3541 cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
     3601 cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag
     3661 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     3721 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     3781 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     3841 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     3901 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcga
     3961 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     4021 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc
     4081 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     4141 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     4201 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     4261 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     4321 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     4381 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     4441 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     4501 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     4561 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     4621 catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     4681 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     4741 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     4801 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     4861 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     4921 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     4981 ctggttcttt ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt
     5041 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5101 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     5161 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5221 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5281 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5341 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5401 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5461 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5521 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt tt
//
