LOCUS       V032395                11285 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032395
VERSION     V032395
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             757..4920
                     /label="ROCK2(NM_004850)"
                     /note="ROCK2(NM_004850)"
                     /gene="ROCK2"
     CDS             5227..5283
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5284..5994
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     misc_feature    6061..6649
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    6715..6763
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6965..7393
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7407..7736
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7803..8597
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8773..8894
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8943..8959)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8967..8983
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8991..9021)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9036..9057
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9345..9933)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10104..10964)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10965..11069)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagc tagcgtttaa acttaagctt gccaccatga gccggccccc gccgacgggg
      781 aaaatgcccg gcgcccccga gaccgcgccg ggggacgggg caggcgcgag ccgccagagg
      841 aagctggagg cgctgatccg agaccctcgc tcccccatca acgtggagag cttgctggat
      901 ggcttaaatt ccttggtcct tgatttagat tttcctgctt tgaggaaaaa caagaacata
      961 gataatttct taaatagata tgagaaaatt gtgaaaaaaa tcagaggtct acagatgaag
     1021 gcagaagact atgatgttgt aaaagttatt ggaagaggtg cttttggtga agtgcagttg
     1081 gttcgtcaca aggcatcgca gaaggtttat gctatgaagc ttcttagtaa gtttgaaatg
     1141 ataaaaagat cagattctgc ctttttttgg gaagaaagag atattatggc ctttgccaat
     1201 agcccctggg tggttcagct tttttatgcc tttcaagatg ataggtatct gtacatggta
     1261 atggagtaca tgcctggtgg agaccttgta aaccttatga gtaattatga tgtgcctgaa
     1321 aaatgggcca aattttacac tgctgaagtt gttcttgctc tggatgcaat acactccatg
     1381 ggtttaatac acagagatgt gaagcctgac aacatgctct tggataaaca tggacatcta
     1441 aaattagcag attttggcac gtgtatgaag atggatgaaa caggcatggt acattgtgat
     1501 acagcagttg gaacaccgga ttatatatca cctgaggttc tgaaatcaca agggggtgat
     1561 ggtttctatg ggcgagaatg tgattggtgg tctgtaggtg ttttccttta tgagatgcta
     1621 gtgggggata ctccatttta tgcggattca cttgtaggaa catatagcaa aattatggat
     1681 cataagaatt cactgtgttt ccctgaagat gcagaaattt ccaaacatgc aaagaatctc
     1741 atctgtgctt tcttaacaga tagggaggta cgacttggga gaaatggggt ggaagaaatc
     1801 agacagcatc ctttctttaa gaatgatcag tggcattggg ataacataag agaaacggca
     1861 gctcctgtag tacctgaact cagcagtgac atagacagca gcaatttcga tgacattgaa
     1921 gatgacaaag gagatgtaga aaccttccca attcctaaag cttttgttgg aaatcagctg
     1981 cctttcatcg gatttaccta ctatagagaa aatttattat taagtgactc tccatcttgt
     2041 agagaaactg attccataca atcaaggaaa aatgaagaaa gtcaagagat tcagaaaaaa
     2101 ctgtatacat tagaagaaca tcttagcaat gagatgcaag ccaaagagga actggaacag
     2161 aagtgcaaat ctgttaatac tcgcctagaa aaaacagcaa aggagctaga agaggagatt
     2221 accttacgga aaagtgtgga atcagcatta agacagttag aaagagaaaa ggcgcttctt
     2281 cagcacaaaa atgcagaata tcagaggaaa gctgatcatg aagcagacaa aaaacgaaat
     2341 ttggaaaatg atgttaacag cttaaaagat caacttgaag atttgaaaaa aagaaatcaa
     2401 aactctcaaa tatccactga gaaagtgaat caactccaga gacaactgga tgaaaccaat
     2461 gctttactgc gaacagagtc tgatactgca gcccggttaa ggaaaaccca ggcagaaagt
     2521 tcaaaacaga ttcagcagct ggaatctaac aatagagatc tacaagataa aaactgcctg
     2581 ctggagactg ccaagttaaa acttgaaaag gaatttatca atcttcagtc agctctagaa
     2641 tctgaaagga gggatcgaac ccatggatca gagataatta atgatttaca aggtagaata
     2701 tgtggcctag aagaagattt aaagaacggc aaaatcttac tagcgaaagt agaactggag
     2761 aagagacaac ttcaggagag atttactgat ttggaaaagg aaaaaagcaa catggaaata
     2821 gatatgacat accaactaaa agttatacag cagagcctag aacaagaaga agctgaacat
     2881 aaggccacaa aggcacgact agcagataaa aataagatct atgagtccat cgaagaagcc
     2941 aaatcagaag ccatgaaaga aatggagaag aagctcttgg aggaaagaac tttaaaacag
     3001 aaagtggaga acctattgct agaagctgag aaaagatgtt ctctattaga ctgtgacctc
     3061 aaacagtcac agcagaaaat aaatgagctc cttaaacaga aagatgtgct aaatgaggat
     3121 gttagaaacc tgacattaaa aatagagcaa gaaactcaga agcgctgcct tacacaaaat
     3181 gacctgaaga tgcaaacaca acaggttaac acactaaaaa tgtcagaaaa gcagttaaag
     3241 caagaaaata accatctcat ggaaatgaaa atgaacttgg aaaaacaaaa tgctgaactt
     3301 cgaaaagaac gtcaggatgc agatgggcaa atgaaagagc tccaggatca gctcgaagca
     3361 gaacagtatt tctcaaccct ttataaaaca caagttaggg agcttaaaga agaatgtgaa
     3421 gaaaagacca aacttggtaa agaattgcag cagaagaaac aggaattaca ggatgaacgg
     3481 gactctttgg ctgcccaact ggagatcacc ttgaccaaag cagattctga gcaactggct
     3541 cgttcaattg ctgaagaaca atattctgat ttggaaaaag agaagatcat gaaagagctg
     3601 gagatcaaag agatgatggc tagacacaaa caggaactta cggaaaaaga tgctacaatt
     3661 gcttctcttg aggaaactaa taggacacta actagtgatg ttgccaatct tgcaaatgag
     3721 aaagaagaat taaataacaa attgaaagat gttcaagagc aactgtcaag attgaaagat
     3781 gaagaaataa gcgcagcagc tattaaagca cagtttgaga agcagctatt aacagaaaga
     3841 acactcaaaa ctcaagctgt gaataagttg gctgagatca tgaatcgaaa agaacctgtc
     3901 aagcgtggta atgacacaga tgtgcggaga aaagagaagg agaatagaaa gctacatatg
     3961 gagcttaaat ctgaacgtga gaaattgacc cagcagatga tcaagtatca gaaagaactg
     4021 aatgaaatgc aggcacaaat agctgaagag agccagattc gaattgaact gcagatgaca
     4081 ttggacagta aagacagtga cattgagcag ctgcggtcac aactccaagc cttgcatatt
     4141 ggtctggata gttccagtat aggcagtgga ccaggggatg ctgaggcaga tgatgggttt
     4201 ccagaatcaa gattagaagg atggctttca ttgcctgtac gaaacaacac taagaaattt
     4261 ggatgggtta aaaagtatgt gattgtaagc agtaagaaga ttcttttcta tgacagtgaa
     4321 caagataaag aacaatccaa tccttacatg gttttagata tagacaagtt atttcatgtc
     4381 cgaccagtta cacagacaga tgtgtataga gcagatgcta aagaaattcc aaggatattc
     4441 cagattctgt atgccaatga aggagaaagt aagaaggaac aagaatttcc agtggagcca
     4501 gttggagaaa aatctaatta tatttgccac aagggacatg agtttattcc tactctttat
     4561 catttcccaa ccaactgtga ggcttgtatg aagcccctgt ggcacatgtt taagcctcct
     4621 cctgctttgg agtgccgccg ttgccatatt aagtgtcata aagatcatat ggacaaaaag
     4681 gaggagatta tagcaccttg caaagtatat tatgatattt caacggcaaa gaatctgtta
     4741 ttactagcaa attctacaga agagcagcag aagtgggtta gtcggttggt gaaaaagata
     4801 cctaaaaagc ccccagctcc agaccctttt gcccgatcat ctcctagaac ttcaatgaag
     4861 atacagcaaa accagtctat tagacggcca agtcgacagc ttgccccaaa caaacctagc
     4921 gaattcgcca ccatgattct ctgggaaaag aaaagcgaag atattaccga gacattcaac
     4981 cccgacaaca acatgatcgc cgccgacgcg agcgaggcat cggaggcggg gaaggcagaa
     5041 aacacccatt acaccggcaa gcaccgcttc atgtaccgga tcctgttcgt cctgacctgc
     5101 ctgatcacct tcattgtggt ggctattgtc ggcggaatta tcgcttcgaa cagaaacgtc
     5161 ggaaatagaa tagctcaagg ccaaggatta gccttgaaat tagcaggtct tgatatcgga
     5221 agcggagcta ctaacttcag cctgctgaag caggctggcg acgtggagga gaaccctgga
     5281 cctatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     5341 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     5401 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     5461 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     5521 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     5581 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     5641 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     5701 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     5761 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     5821 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     5881 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     5941 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaactcgag
     6001 tctagagggc cccttaaggg ttcgatccct accggttagt aatgagtttg atatctcgac
     6061 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6121 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6181 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6241 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6301 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6361 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6421 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     6481 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6541 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6601 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaaacggg
     6661 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     6721 taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg
     6781 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     6841 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     6901 aacgtcgggg cggcaggccc tgccatagca gatctgcgca gctggggctc tagggggtat
     6961 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7021 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7081 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga
     7141 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7201 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7261 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7321 ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7381 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     7441 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     7501 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7561 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7621 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     7681 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     7741 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     7801 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     7861 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     7921 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     7981 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     8041 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     8101 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     8161 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     8221 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     8281 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     8341 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     8401 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     8461 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     8521 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     8581 ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc
     8641 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8701 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     8761 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8821 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8881 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     8941 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9001 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9061 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9121 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9181 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9241 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9301 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9361 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9421 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9481 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     9541 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9601 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9661 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9721 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9781 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9841 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9901 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9961 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10021 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10081 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10141 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10201 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10261 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10321 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10381 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10441 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10501 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10561 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10621 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10681 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10741 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10801 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10861 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10921 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10981 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    11041 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    11101 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
    11161 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
    11221 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
    11281 ttagg
//
