LOCUS       V023271                 8183 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023271
VERSION     V023271
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1055..4345
                     /label="REST(NM_005612)"
                     /note="REST(NM_005612)"
                     /gene="REST"
     CDS             4349..4378
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             4397..4420
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(4440..4456)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    4467..5089
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(5238..5826)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    6155..6202
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(6434..7228)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(7263..7620)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(7622..7726)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7753..8181
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgcggc gcgccagatc tcaagcttgc caccatggcc acccaggtaa tggggcagtc
     1081 ttctggagga ggagggctgt ttaccagcag tggcaacatt ggaatggccc tgcctaacga
     1141 catgtatgac ttgcatgacc tttccaaagc tgaactggcc gcacctcagc ttattatgct
     1201 ggcaaatgtg gccttaactg gggaagtaaa tggcagctgc tgtgattacc tggtcggtga
     1261 agaaagacag atggcagaac tgatgccggt tggggataac aacttttcag atagtgaaga
     1321 aggagaagga cttgaagagt ctgctgatat aaaaggtgaa cctcatggac tggaaaacat
     1381 ggaactgaga agtttggaac tcagcgtcgt agaacctcag cctgtatttg aggcatcagg
     1441 tgctccagat atttacagtt caaataaaga tcttccccct gaaacacctg gagcggagga
     1501 caaaggcaag agctcgaaga ccaaaccctt tcgctgtaag ccatgccaat atgaagcaga
     1561 atctgaagaa cagtttgtgc atcacatcag agttcacagt gctaagaaat tttttgtgga
     1621 agagagtgca gagaagcagg caaaagccag ggaatctggc tcttccactg cagaagaggg
     1681 agatttctcc aagggcccca ttcgctgtga ccgctgcggc tacaatacta atcgatatga
     1741 tcactataca gcacacctga aacaccacac cagagctggg gataatgagc gagtctacaa
     1801 gtgtatcatt tgcacataca caacagtgag cgagtatcac tggaggaaac atttaagaaa
     1861 ccattttcca aggaaagtat acacatgtgg aaaatgcaac tatttttcag acagaaaaaa
     1921 caattatgtt cagcatgtta gaactcatac aggagaacgc ccatataaat gtgaactttg
     1981 tccttactca agttctcaga agactcatct aactagacat atgcgtactc attcaggtga
     2041 gaagccattt aaatgtgatc agtgcagtta tgtggcctct aatcaacatg aagtaacccg
     2101 ccatgcaaga caggttcaca atgggcctaa acctcttaat tgcccacact gtgattacaa
     2161 aacagcagat agaagcaact tcaaaaaaca tgtagagcta catgtgaacc cacggcagtt
     2221 caattgccct gtatgtgact atgcagcttc caagaagtgt aatctacagt atcacttcaa
     2281 atctaagcat cctacttgtc ctaataaaac aatggatgtc tcaaaagtga aactaaagaa
     2341 aaccaaaaaa cgagaggctg acttgcctga taatattacc aatgaaaaaa cagaaataga
     2401 acaaacaaaa ataaaagggg atgtggctgg aaagaaaaat gaaaagtccg tcaaagcaga
     2461 gaaaagagat gtctcaaaag agaaaaagcc ttctaataat gtgtcagtga tccaggtgac
     2521 taccagaact cgaaaatcag taacagaggt gaaagagatg gatgtgcata caggaagcaa
     2581 ttcagaaaaa ttcagtaaaa ctaagaaaag caaaaggaag ctggaagttg acagccattc
     2641 tttacatggt cctgtgaatg atgaggaatc ttcaacaaaa aagaaaaaga aggtagaaag
     2701 caaatccaaa aataatagtc aggaagtgcc aaagggtgac agcaaagtgg aggagaataa
     2761 aaagcaaaat acttgcatga aaaaaagtac aaagaagaaa actctgaaaa ataaatcaag
     2821 taagaaaagc agtaagcctc ctcagaagga acctgttgag aagggatctg ctcagatgga
     2881 ccctcctcag atggggcctg ctcccacaga ggcggttcag aaggggcccg ttcaggtgga
     2941 gccgccacct cccatggagc atgctcagat ggagggtgcc cagatacggc ctgctcctga
     3001 cgagcctgtt cagatggagg tggttcagga ggggcctgct cagaaggagc tgctgcctcc
     3061 cgtggagcct gctcagatgg tgggtgccca aattgtactt gctcacatgg agctgcctcc
     3121 tcccatggag actgctcaga cggaggttgc ccaaatgggg cctgctccca tggaacctgc
     3181 tcagatggag gttgcccagg tagaatctgc tcccatgcag gtggtccaga aggagcctgt
     3241 tcagatggag ctgtctcctc ccatggaggt ggtccagaag gagcctgttc agatagagct
     3301 gtctcctccc atggaggtgg tccagaagga acctgttaag atagagctgt ctcctcccat
     3361 agaggtggtc cagaaggagc ctgttcagat ggagttgtct cctcccatgg gggtggttca
     3421 gaaggagcct gctcagaggg agccacctcc tcccagagag cctccccttc acatggagcc
     3481 aatttccaaa aagcctcctc tccgaaaaga taaaaaggaa aagtctaaca tgcagagtga
     3541 aagggcacgg aaggagcaag tccttattga agttggctta gtgcctgtta aagatagctg
     3601 gcttctaaag gaaagtgtaa gcacagagga tctctcacca ccatcaccac cactgccaaa
     3661 ggaaaattta agagaagagg catcaggaga ccaaaaatta ctcaacacag gtgaaggaaa
     3721 taaagaagcc cctcttcaga aagtaggagc agaagaggca gatgagagcc tacctggtct
     3781 tgctgctaat atcaacgaat ctacccatat ttcatcctct ggacaaaact tgaatacgcc
     3841 agagggtgaa actttaaatg gtaaacatca gactgacagt atagtttgtg aaatgaaaat
     3901 ggacactgat cagaacacaa gagagaatct cactggtata aattcaacag ttgaagaacc
     3961 agtttcacca atgcttcccc cttcagcagt agaagaacgt gaagcagtgt ccaaaactgc
     4021 actggcatca cctcctgcta caatggcagc aaatgagtct caggaaattg atgaagatga
     4081 aggcatccac agccatgaag gaagtgacct aagtgacaac atgtcagagg gtagtgatga
     4141 ttctggattg catggggctc ggccagttcc acaagaatct agcagaaaaa atgcaaagga
     4201 agccttggca gtcaaagcgg ctaagggaga ttttgtttgt atcttctgtg atcgttcttt
     4261 cagaaaggga aaagattaca gcaaacacct caatcgccat ttggttaatg tgtactatct
     4321 tgaagaagca gctcaagggc aggagctcga gcagaaactc atctcagaag aggatctggc
     4381 agcaaatgat atcctggatt acaaggatga cgacgataag gtttaaacgg ccggccgcgg
     4441 tcatagctgt ttcctgaaca gatcccgggt ggcatccctg tgacccctcc ccagtgcctc
     4501 tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt
     4561 gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat
     4621 ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa
     4681 gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga
     4741 ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct
     4801 aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact
     4861 cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa
     4921 ccactgctcc cttccctgtc cttctgattt taaaataact ataccagcag gaggacgtcc
     4981 agacacagca taggctacct ggccatgccc aaccggtggg acatttgagt tgcttgcttg
     5041 gcactgtcct ctcatgcgtt gggtccactc agtagatgcc tgttgaattg ggtacgcggc
     5101 cagcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5161 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5221 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5281 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5341 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5401 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5461 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5521 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5581 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5641 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5701 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5761 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5821 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5881 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5941 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aacctgaggc tatggcaggg
     6001 cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg ggggtggggt
     6061 ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca
     6121 gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt
     6181 ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt ctccttccgt
     6241 gtttcagtta gcctccccct agggtgggcg aagaactcca gcatgagatc cccgcgctgg
     6301 aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg
     6361 gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac
     6421 cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
     6481 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
     6541 cagcaatatc acgggtagcc aacgctatgt cctgatagcg atccgccaca cccagccggc
     6601 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
     6661 cgccatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca
     6721 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
     6781 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
     6841 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
     6901 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
     6961 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
     7021 gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct
     7081 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
     7141 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
     7201 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga
     7261 tctttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     7321 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     7381 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     7441 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     7501 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     7561 cctggggact ttccacaccc taactgacac acattccaca gctggttctt tccgcctcag
     7621 gactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7681 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7741 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     7801 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     7861 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7921 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     7981 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     8041 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     8101 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     8161 aacaaaaatt taacgcgaat ttt
//
