LOCUS       V030583                 8343 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030583
VERSION     V030583
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..4490
                     /label="Ptprc(NM_011210)"
                     /note="Ptprc(NM_011210)"
                     /gene="Ptprc"
     CDS             4509..4538
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             4557..4580
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(4600..4616)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    4627..5249
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(5398..5986)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    6315..6362
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(6594..7388)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(7423..7780)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(7782..7886)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7913..8341
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaccatgggt ttgtggctca aacttctggc ctttggattt gcccttctgg
     1081 acacagaagt ctttgtcaca gggcaaacac ctacacccag tgatggtgcc agcctcacaa
     1141 ctcttacacc atccactctg ggccttgcaa gcactgaccc tccaagcaca accatagcta
     1201 ccacaacgaa gcaaacatgt gctgccatgt ttgggaacat tactgtgaat tacacctatg
     1261 aatctagtaa tcagactttt aaggcagacc tcaaagatgt ccaaaatgct aagtgtggaa
     1321 atgaggattg tgaaaacgtg ttaaataatc tagaagaatg ctcacagata aaaaacatca
     1381 gtgtgtctaa tgactcatgt gctccagcta caactataga tttatatgta ccaccaggga
     1441 ctgacaagtt ttcgctacat gactgcacac caaaagaaaa ggctaatact tcaatttgtt
     1501 tggagtggaa aacaaaaaac cttgatttca gaaaatgcaa cagtgacaat atttcatatg
     1561 tactccactg tgagccagaa aataatacaa aatgcattag aagaaataca ttcatacctg
     1621 aaagatgtca gttggacaac cttcgtgccc aaacaaatta cacatgtgta gcagaaatct
     1681 tatatcgcgg tgtaaaactc gtcaaaaatg ttataaatgt gcagacagat ttggggattc
     1741 cagaaacgcc taagcctagt tgtggggatc cagctgcaag aaaaacgtta gtctcttggc
     1801 ctgagcctgt atctaaacct gagtctgcat ctaaacccca tggatatgtt ttatgctata
     1861 agaacaattc agaaaaatgt aaaagtttgc ctaataatgt gaccagtttt gaggtggaaa
     1921 gcttgaaacc ttataaatac tatgaagtgt ccctacttgc ctatgtcaat gggaagattc
     1981 aaagaaatgg gactgctgag aagtgcaatt ttcacacaaa agcagatcgt ccggacaagg
     2041 tcaatggaat gaaaacctcc cggccgacag acaatagtat aaatgttaca tgtggtcctc
     2101 cttatgaaac taatggccct aaaacctttt acattttggt agtcagaagt ggaggttctt
     2161 ttgttacaaa atacaacaag acaaactgtc agttttatgt agataatctc tactattcaa
     2221 ctgactatga gtttctggtc tcttttcaca atggagtgta cgagggagat tcagttataa
     2281 gaaatgagtc aacaaatttt aatgctaaag cactgattat attcctggtg tttctgatta
     2341 ttgtgacatc aatagccttg cttgttgttt tgtataaaat ctatgatctg cgcaagaaaa
     2401 gatccagcaa tttagatgaa caacaggaac tcgttgaaag ggatgatgaa aagcagctga
     2461 tggatgtgga gccaatccat tctgacattt tgttggaaac atacaaaagg aagattgctg
     2521 atgagggcag actgttcctg gctgaatttc agagcattcc acgggtattc agcaagtttc
     2581 ccatcaaaga tgcccgaaag ccccacaatc agaataaaaa ccgttatgtt gacattcttc
     2641 cctatgatta taaccgtgtg gaactctctg aaataaatgg agatgcaggg tccacctaca
     2701 taaatgccag ctacattgat ggcttcaagg aacccaggaa atacattgct gcacaagggc
     2761 cccgggatga gacagttgat gacttctgga ggatgatctg ggagcaaaag gccacagtta
     2821 ttgtcatggt cacacgatgt gaagaaggaa acaggaacaa gtgcgcagaa tactggccaa
     2881 gcatggagga aggcactcgg gctttcaaag atattgttgt gacaatcaat gaccacaaac
     2941 gatgtcctga ttacatcatt cagaagctga acgttgcaca taaaaaagaa aaagcaactg
     3001 gaagagaagt gactcatatc caattcacca gctggccaga ccatggggtt cctgaagacc
     3061 ctcacctgct cctcaaactt cgacggagag ttaatgcttt tagcaacttc ttcagtggtc
     3121 ccattgtggt gcactgcagt gctggtgttg ggcgtacagg tacctacatt ggaattgatg
     3181 ccatgctgga aggcctggaa gcagagggca aagtggatgt ctatggttat gttgtcaagc
     3241 taaggcgaca gaggtgtctg atggtgcaag tggaggcaca gtatatcctg attcatcagg
     3301 ctttagtgga atacaatcag tttggagaaa cagaagtgaa cttgtctgag ttacattcat
     3361 gcctacacaa catgaagaag agagatccac ccagtgaccc ctcccctctg gaggctgaat
     3421 accagagact tccttcatac aggagttgga ggacacagca cattggaaat caagaagaaa
     3481 ataagaagaa gaacaggaat tctaatgttg ttccatatga ctttaacaga gtgccactta
     3541 agcatgaact ggagatgagc aaagagagtg agcctgaatc agatgagtct tcagatgatg
     3601 acagtgactc agaagaaacc agcaaataca ttaatgcatc ctttgtgatg agttactgga
     3661 aaccagaaat gatgattgct gctcaggggc cactaaaaga aacgatcggt gacttttggc
     3721 agatgatatt ccaaagaaaa gtcaaagtta ttgtgatgtt gacagagtta gtgaatggag
     3781 accaggaagt ctgtgctcag tactggggcg aaggaaagca gacttatgga gacatggaag
     3841 tggagatgaa agacacaaac agagcctcag cctacactct ccgaactttt gagctgagac
     3901 attccaagag gaaggagccc agaactgtgt accagtacca gtgtaccaca tggaaagggg
     3961 aagagctgcc tgcagaaccc aaagacctgg tgtctatgat tcaggacctc aaacagaagc
     4021 ttcccaaggc ttccccagaa gggatgaagt atcacaagca tgcatccatc ctcgtccact
     4081 gcagagatgg atcccagcag acagggttgt tctgtgcctt gttcaatctc ttggaaagtg
     4141 cagaaacaga agatgtggtt gatgttttcc aagtggtaaa gtctctacgc aaagcacggc
     4201 ctggggtggt gtgcagctat gagcaatacc agttcctcta tgacatcatc gccagcatct
     4261 atcccgccca gaatggacaa gtcaagaaaa caaacagcca agacaaaatt gaatttcata
     4321 atgaagtgga tggaggcaag caggatgcta actgtgtccg tccagatggt cctctgaata
     4381 aagcccagga agacagcaga ggggtgggaa ccccggagcc taccaatagt gctgaggaac
     4441 cagaacatgc tgccaatggt tctgcgagcc cagctccaac ccagagttca acgcgtacgc
     4501 ggccgctcga gcagaaactc atctcagaag aggatctggc agcaaatgat atcctggatt
     4561 acaaggatga cgacgataag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca
     4621 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
     4681 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     4741 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
     4801 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
     4861 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
     4921 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
     4981 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
     5041 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     5101 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct
     5161 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt
     5221 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcggcgag cggtatcagc
     5281 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5341 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5401 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5461 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5521 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5581 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5641 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5701 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5761 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5821 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5881 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5941 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6001 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6061 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6121 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc
     6181 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg
     6241 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg
     6301 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc
     6361 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct
     6421 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
     6481 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     6541 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
     6601 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     6661 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     6721 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa
     6781 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     6841 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     6901 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     6961 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     7021 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     7081 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     7141 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     7201 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     7261 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     7321 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     7381 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc
     7441 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     7501 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     7561 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     7621 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     7681 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     7741 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat
     7801 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7861 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     7921 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7981 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8041 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8101 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8161 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8221 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8281 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8341 ttt
//
