LOCUS V029545 6354 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029545 VERSION V029545 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..2492 /label="Ptger4(NM_001136079)" /note="Ptger4(NM_001136079)" /gene="Ptger4" CDS 2520..2549 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2568..2591 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2611..2627) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2638..3260 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(3409..3997) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 4326..4373 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4605..5399) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(5434..5791) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5793..5897) /gene="bla" /label="AmpR promoter" rep_origin 5924..6352 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gtccatcccc ggagtcaacg cgtccttctc ctccactccg gagaggctga 1081 acagcccggt gaccattccc gcagtgatgt tcatcttcgg ggtggtgggc aacctggtgg 1141 ccatcgtagt attgtgcaag tcgcgcaagg agcagaaaga gacgaccttt tacactctag 1201 tatgtgggct ggctgtcact gaccttctgg gcaccttgtt ggtaagcccg gtgaccatcg 1261 ccacatacat gaagggccag tggcccggag accaggcact gtgtgactat agcaccttca 1321 tcctactttt cttcggtctg tcgggtctca gcatcatctg tgccatgagc atcgagcgct 1381 acctggccat caaccacgcc tacttctaca gccactacgt ggacaagcgg ctggccggcc 1441 tcacactctt cgccatctat gcatctaacg tgctgttctg cgcgctgccc aacatgggcc 1501 tgggcagatc cgagcggcag tacccgggca cctggtgctt catcgactgg accaccaacg 1561 taacggccta cgccgccttc tcttacatgt acgccggctt cagctccttc ctcatccttg 1621 ccaccgtgct ctgcaacgtg ctggtgtgcg gcgcgctgct ccgcatgcac cgccagttca 1681 tgcgccgcac ctcgttgggc acggagcagc accatgcggc tgccgccgcc gcggtagctt 1741 cggtggcctg tcggggccac gctggggcct ccccagccct gcagcgcctc agcgactttc 1801 gccgccgcag gagtttccgg cgcatcgcgg gtgcggagat ccagatggtc atcttactca 1861 tcgccacctc tctggtggtg ctcatctgct ccattccgct cgtggtgcga gtgttcatta 1921 accagttata tcagccaaac gtggtgaaag acatcagcag aaacccagat ttgcaggcca 1981 tcaggattgc ttctgtgaac cccatcctgg acccctggat ttacatcctt cttcggaaga 2041 ctgtgctcag taaagccata gagaagatca agtgcctctt ctgccgcatt ggcggttccg 2101 gcagagacag ctcggcccag cactgctcag agagtcggag gacatcttcc gccatgtccg 2161 gccactctcg ctccttcctc gcccgggagt taaaggagat cagcagcacg tcccagaccc 2221 tcctgtacct gccagacctg actgaaagca gcctcggagg caggaatttg cttccaggtt 2281 cgcatggcat gggcctgacc caagcagaca ccacctcgct gagaactttg cgaatttccg 2341 agacctcaga ctcctcccag ggccaggact ctgagagtgt cctgttggtg gatgaggtta 2401 gtgggagcca cagagaggag cctgcctcta aaggaaactc tctgcaagtc acattcccca 2461 gtgaaactct gaaattatct gaaaaatgta taagcggacc gacgcgtacg cggccgctcg 2521 agcagaaact catctcagaa gaggatctgg cagcaaatga tatcctggat tacaaggatg 2581 acgacgataa ggtttaaacg gccggccgcg gtcatagctg tttcctgaac agatcccggg 2641 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg 2701 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc 2761 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc 2821 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc 2881 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg 2941 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt 3001 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg 3061 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt 3121 ttaaaataac tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc 3181 caaccggtgg gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact 3241 cagtagatgc ctgttgaatt gggtacgcgg ccagcggcga gcggtatcag ctcactcaaa 3301 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 3361 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 3421 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 3481 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 3541 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 3601 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 3661 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 3721 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 3781 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 3841 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 3901 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 3961 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 4021 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 4081 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 4141 tatatatgag taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc 4201 tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg 4261 gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg 4321 tttatgaaca aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc 4381 gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc 4441 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac 4501 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag 4561 gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca 4621 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg 4681 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg 4741 tcctgatagc gatccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca 4801 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg 4861 tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct 4921 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg 4981 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc 5041 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc 5101 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc 5161 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc 5221 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct 5281 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg 5341 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg 5401 cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa 5461 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat 5521 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg 5581 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat 5641 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg 5701 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca 5761 cacattccac agctggttct ttccgcctca ggactcttcc tttttcaata ttattgaagc 5821 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 5881 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc 5941 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 6001 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 6061 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 6121 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 6181 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 6241 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 6301 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa tttt //