LOCUS       V029545                 6354 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029545
VERSION     V029545
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2492
                     /label="Ptger4(NM_001136079)"
                     /note="Ptger4(NM_001136079)"
                     /gene="Ptger4"
     CDS             2520..2549
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2568..2591
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2611..2627)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2638..3260
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3409..3997)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4326..4373
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4605..5399)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5434..5791)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5793..5897)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5924..6352
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gtccatcccc ggagtcaacg cgtccttctc ctccactccg gagaggctga
     1081 acagcccggt gaccattccc gcagtgatgt tcatcttcgg ggtggtgggc aacctggtgg
     1141 ccatcgtagt attgtgcaag tcgcgcaagg agcagaaaga gacgaccttt tacactctag
     1201 tatgtgggct ggctgtcact gaccttctgg gcaccttgtt ggtaagcccg gtgaccatcg
     1261 ccacatacat gaagggccag tggcccggag accaggcact gtgtgactat agcaccttca
     1321 tcctactttt cttcggtctg tcgggtctca gcatcatctg tgccatgagc atcgagcgct
     1381 acctggccat caaccacgcc tacttctaca gccactacgt ggacaagcgg ctggccggcc
     1441 tcacactctt cgccatctat gcatctaacg tgctgttctg cgcgctgccc aacatgggcc
     1501 tgggcagatc cgagcggcag tacccgggca cctggtgctt catcgactgg accaccaacg
     1561 taacggccta cgccgccttc tcttacatgt acgccggctt cagctccttc ctcatccttg
     1621 ccaccgtgct ctgcaacgtg ctggtgtgcg gcgcgctgct ccgcatgcac cgccagttca
     1681 tgcgccgcac ctcgttgggc acggagcagc accatgcggc tgccgccgcc gcggtagctt
     1741 cggtggcctg tcggggccac gctggggcct ccccagccct gcagcgcctc agcgactttc
     1801 gccgccgcag gagtttccgg cgcatcgcgg gtgcggagat ccagatggtc atcttactca
     1861 tcgccacctc tctggtggtg ctcatctgct ccattccgct cgtggtgcga gtgttcatta
     1921 accagttata tcagccaaac gtggtgaaag acatcagcag aaacccagat ttgcaggcca
     1981 tcaggattgc ttctgtgaac cccatcctgg acccctggat ttacatcctt cttcggaaga
     2041 ctgtgctcag taaagccata gagaagatca agtgcctctt ctgccgcatt ggcggttccg
     2101 gcagagacag ctcggcccag cactgctcag agagtcggag gacatcttcc gccatgtccg
     2161 gccactctcg ctccttcctc gcccgggagt taaaggagat cagcagcacg tcccagaccc
     2221 tcctgtacct gccagacctg actgaaagca gcctcggagg caggaatttg cttccaggtt
     2281 cgcatggcat gggcctgacc caagcagaca ccacctcgct gagaactttg cgaatttccg
     2341 agacctcaga ctcctcccag ggccaggact ctgagagtgt cctgttggtg gatgaggtta
     2401 gtgggagcca cagagaggag cctgcctcta aaggaaactc tctgcaagtc acattcccca
     2461 gtgaaactct gaaattatct gaaaaatgta taagcggacc gacgcgtacg cggccgctcg
     2521 agcagaaact catctcagaa gaggatctgg cagcaaatga tatcctggat tacaaggatg
     2581 acgacgataa ggtttaaacg gccggccgcg gtcatagctg tttcctgaac agatcccggg
     2641 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     2701 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     2761 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     2821 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     2881 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     2941 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     3001 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     3061 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
     3121 ttaaaataac tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc
     3181 caaccggtgg gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact
     3241 cagtagatgc ctgttgaatt gggtacgcgg ccagcggcga gcggtatcag ctcactcaaa
     3301 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3361 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3421 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3481 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3541 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3601 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3661 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3721 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3781 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3841 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3901 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3961 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4021 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4081 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4141 tatatatgag taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc
     4201 tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg
     4261 gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg
     4321 tttatgaaca aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc
     4381 gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc
     4441 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac
     4501 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag
     4561 gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca
     4621 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     4681 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     4741 tcctgatagc gatccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     4801 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg
     4861 tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     4921 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     4981 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     5041 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     5101 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     5161 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc
     5221 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     5281 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     5341 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     5401 cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa
     5461 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     5521 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     5581 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     5641 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     5701 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     5761 cacattccac agctggttct ttccgcctca ggactcttcc tttttcaata ttattgaagc
     5821 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5881 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
     5941 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6001 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6061 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6121 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6181 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6241 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6301 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa tttt
//
