LOCUS       V030938                 5904 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030938
VERSION     V030938
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2051
                     /label="Ptafr(NM_001081211)"
                     /note="Ptafr(NM_001081211)"
                     /gene="Ptafr"
     CDS             2070..2099
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2118..2141
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2161..2177)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2188..2810
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2959..3547)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3876..3923
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4155..4949)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4984..5341)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5343..5447)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5474..5902
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagcacaat ggctcctttc gtgtggattc tgagtttcga tacacgctct
     1081 ttccgattgt ttacagtgtc atctttatac tgggggtggt tgccaacggc tatgtgctat
     1141 gggtctttgc taacttgtac ccttccaaga aactaaatga gataaagatc tttatggtga
     1201 atctcactat ggctgacctg ctcttcctga tcaccctccc actgtggatt gtctactact
     1261 acaacgaggg cgactggatt ctacccaact tcctgtgcaa cgtggctggc tgcctcttct
     1321 tcatcaatac ctactgcagt gtggcctttt tgggtgtcat cacttataac cgctaccagg
     1381 cagtagccta tcccatcaag actgcacagg ccaccacccg caagcgtggc atctctttgt
     1441 ccctgatcat ttgggtatcc attgtggcta ctgcatccta tttcctggcc acagactcca
     1501 ccaacctagt gcccaataag gatggctcag gcaacatcac ccgctgcttt gagcattatg
     1561 agccatacag tgtgcccatc cttgttgttc atgtcttcat cgccttctgc ttcttcctcg
     1621 tcttcttcct tatcttctac tgcaacttgg tcatcatcca cacgctgctc acgcagccca
     1681 tgaggcagca gcgcaaagcg ggggtgaagc ggagggcgct gtggatggtc tgcacggtct
     1741 tggcggtatt catcatctgt tttgtgcccc atcacgtggt ccagctgccc tggaccctag
     1801 cagagttggg ctaccagacc aacttccatc aggctattaa tgatgcccat caaatcaccc
     1861 tctgcctcct cagcaccaac tgtgtcttag atcccgttat ctattgcttt cttaccaaga
     1921 agttccgaaa gcacctcagt gagaagtttt acagcatgcg cagtagccgg aagtgctcca
     1981 gagccacgag tgacacgtgc accgaggtga tagtgccagc caaccagact cctattgtgt
     2041 cgctgaaaaa tacgcgtacg cggccgctcg agcagaaact catctcagaa gaggatctgg
     2101 cagcaaatga tatcctggat tacaaggatg acgacgataa ggtttaaacg gccggccgcg
     2161 gtcatagctg tttcctgaac agatcccggg tggcatccct gtgacccctc cccagtgcct
     2221 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     2281 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     2341 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     2401 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     2461 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     2521 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     2581 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     2641 accactgctc ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc
     2701 cagacacagc ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt
     2761 ggcactgtcc tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg
     2821 ccagcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2881 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2941 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3001 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3061 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3121 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3181 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3241 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3301 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3361 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3421 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3481 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3541 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3601 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3661 ttaaaaatga agttttaaat caatctaaag tatatatgag taacctgagg ctatggcagg
     3721 gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg gggggtgggg
     3781 tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg gggtatcgac
     3841 agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa caccgtgcgt
     3901 tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg
     3961 tgtttcagtt agcctccccc tagggtgggc gaagaactcc agcatgagat ccccgcgctg
     4021 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     4081 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     4141 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     4201 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     4261 tcagcaatat cacgggtagc caacgctatg tcctgatagc gatccgccac acccagccgg
     4321 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     4381 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac
     4441 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     4501 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     4561 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     4621 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     4681 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     4741 agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc
     4801 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     4861 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     4921 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatcg
     4981 atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     5041 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     5101 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     5161 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     5221 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     5281 gcctggggac tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca
     5341 ggactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5401 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5461 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5521 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5581 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5641 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5701 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5761 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5821 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5881 taacaaaaat ttaacgcgaa tttt
//
