LOCUS       V029328                 7002 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029328
VERSION     V029328
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3149
                     /label="Prkcq(NM_008859)"
                     /note="Prkcq(NM_008859)"
                     /gene="Prkcq"
     CDS             3168..3197
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3216..3239
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3259..3275)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3286..3908
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4057..4645)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4974..5021
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5253..6047)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6082..6439)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6441..6545)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6572..7000
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gtcaccgttt cttcgaatcg gtttatccaa ctttgactgt gggacctgcc
     1081 aagcttgtca gggagaggca gtgaacccct actgcgctgt gcttgtcaaa gagtatgtgg
     1141 aatcagaaaa tgggcagatg tacatccaga aaaagccaac catgtaccca ccttgggaca
     1201 gcacctttga cgcccacatt aacaagggaa gggtgatgca gatcatcgtg aagggcaaga
     1261 atgtagacct catctcagaa acaaccgtgg aactctactc cctggcggag agatgccgca
     1321 agaacaatgg gcggacagaa atatggttag agctgaaacc tcaaggccga atgctaatga
     1381 atgcaagata ctttctggaa atgagtgaca caaaggacat gagtgagttt gagaatgaag
     1441 gattctttgc actgcatcag cgccgaggag ccatcaaaca ggccaaagtc caccatgtca
     1501 agtgtcacga gttcacggcc acctttttcc ctcaacccac attttgctct gtctgccatg
     1561 aatttgtctg gggcctgaac aagcagggtt accagtgccg acagtgtaat gcagcgattc
     1621 acaagaagtg cattgataaa gtgatagcca agtgcacagg atccgcaatc aatagccgag
     1681 aaaccatgtt ccataaggag agattcaaga tcgacatgcc acacagattc aaagtctaca
     1741 actacaagag tccaaccttc tgtgagcact gtggtaccct gctctggggg ctggcgaggc
     1801 aaggactcaa atgtgatgca tgtggcatga acgtccacca ccgatgccag acaaaggttg
     1861 ccaatctttg tggtataaac cagaagctaa tggctgaagc actagcgatg attgaaagca
     1921 cccaacaggc tcgctcctta cgagattcag aacacatctt ccgagaaggc ccagttgaaa
     1981 ttggtctccc atgctccacc aaaaacgaaa ccaggccacc atgcgtacca acacctggga
     2041 aaagagaacc ccagggcatt tcctgggatt cccctttgga tgggtcaaat aaatcggccg
     2101 gtcctcctga acccgaagtg agcatgcgca ggacttcact gcagctgaaa ctgaagatcg
     2161 atgacttcat cctgcacaag atgttgggaa aaggaagttt tggcaaggtc ttcctggcag
     2221 agttcaagag aaccaatcag tttttcgcaa taaaagcctt aaagaaagat gtggtgttga
     2281 tggatgatga cgtcgagtgt acaatggtgg aaaagagggt tctgtccttg gcatgggagc
     2341 atccatttct aacacacatg ttctgcacat tccagaccaa ggaaaatctc tttttcgtga
     2401 tggagtatct caatggaggc gacttaatgt accacatcca aagttgccac aaatttgatc
     2461 tttccagagc cacgttttat gctgctgagg tcatccttgg tctgcagttc cttcattcca
     2521 aaggaattgt ctacagggac ctgaagcttg ataatatcct gttagacaga gatggacata
     2581 tcaaaatagc agactttggg atgtgcaaag agaacatgct aggagatgcg aagacaaata
     2641 ctttctgtgg aactcctgac tacattgctc cggagatctt gctgggtcag aagtacaacc
     2701 attccgtcga ctggtggtcc ttcggggtgc tcgtttatga gatgctgatt ggccagtccc
     2761 ccttccacgg gcaggacgag gaggagctgt tccactccat ccgcatggac aaccccttct
     2821 acccgaggtg gctcgaaagg gaggccaagg accttctagt gaagcttttt gtgagagaac
     2881 ctgagaagag gctgggagtg agaggagaca tccgccagca tcctttgttt cgagagatca
     2941 actgggaaga gcttgaaagg aaagagattg acccaccctt cagaccaaaa gtgaaatcac
     3001 catatgactg tagcaatttc gacaaggaat tcctaagtga gaaaccccgg ctatcattcg
     3061 ccgacagagc actcatcaac agcatggacc agaacatgtt cagcaacttt tccttcatta
     3121 acccagggat ggagactctc atttgctcca cgcgtacgcg gccgctcgag cagaaactca
     3181 tctcagaaga ggatctggca gcaaatgata tcctggatta caaggatgac gacgataagg
     3241 tttaaacggc cggccgcggt catagctgtt tcctgaacag atcccgggtg gcatccctgt
     3301 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     3361 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     3421 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     3481 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     3541 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     3601 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     3661 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     3721 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta
     3781 taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga
     3841 catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct
     3901 gttgaattgg gtacgcggcc agcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3961 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4021 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4081 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4141 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4201 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4261 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4321 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4381 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4441 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4501 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4561 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4621 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4681 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4741 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4801 acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc
     4861 cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg
     4921 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
     4981 cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
     5041 cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag
     5101 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     5161 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     5221 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     5281 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     5341 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcga
     5401 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     5461 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc
     5521 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     5581 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     5641 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     5701 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     5761 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     5821 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     5881 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     5941 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     6001 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     6061 catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     6121 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     6181 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     6241 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     6301 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     6361 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     6421 ctggttcttt ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt
     6481 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     6541 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     6601 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6661 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6721 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6781 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6841 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6901 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6961 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt tt
//
