LOCUS       V030882                 7092 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030882
VERSION     V030882
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3239
                     /label="Prkce(NM_011104)"
                     /note="Prkce(NM_011104)"
                     /gene="Prkce"
     CDS             3258..3287
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3306..3329
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3349..3365)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3376..3998
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4147..4735)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5064..5111
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5343..6137)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6172..6529)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6531..6635)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6662..7090
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggtagtgttc aatggccttc ttaagatcaa aatctgcgag gcggtgagct
     1081 tgaagcccac agcctggtcg ctgcgccatg cggtgggacc ccggccacag acgttccttt
     1141 tggaccccta cattgccctt aacgtggacg actcgcgcat cggccaaaca gccaccaagc
     1201 aaaagaccaa cagcccggcc tggcacgatg agttcgtcac cgatgtgtgc aatgggcgca
     1261 agatcgagct ggctgtcttt cacgacgctc ctatcggcta cgacgacttc gtggccaact
     1321 gcaccatcca gttcgaggag ctgctgcaga atgggagccg tcacttcgag gactggattg
     1381 acctggagcc agaaggaaaa gtgtacgtga tcatcgatct ctcgggatca tcgggtgaag
     1441 cccctaaaga caatgaagaa cgagtgttca gggagcgtat gcggccaagg aagcggcaag
     1501 gggctgtcag gcgcagggtc caccaggtca atggccacaa gttcatggcc acctacttgc
     1561 ggcaacccac ctactgctcc cactgcagag atttcatctg gggtgtcata ggaaaacagg
     1621 gatatcaatg tcaagtttgc acttgcgttg tccacaagcg atgtcatgag ctcattatta
     1681 caaagtgcgc tgggctgaag aaacaggaaa cccctgacga ggtgggctcc caacggttca
     1741 gcgtcaacat gccccacaag ttcgggatcc acaactacaa ggtccccacg ttctgtgacc
     1801 actgtgggtc cctgctctgg ggcctcttgc ggcagggctt gcagtgtaaa gtctgcaaaa
     1861 tgaatgttca ccggcgatgt gagaccaatg tggctcccaa ctgtggggta gacgccagag
     1921 gaattgccaa agtgctggct gaccttggtg ttactccaga caaaatcacc aacagtggcc
     1981 aaaggaggaa aaagctcgct gctggtgctg agtccccaca gccggcttct ggaaactccc
     2041 catctgaaga cgaccgatcc aagtcagcgc ccacctcccc ttgtgaccag gaactaaaag
     2101 aacttgaaaa caacattcgg aaggccttgt catttgacaa ccgaggagag gagcaccgag
     2161 cgtcgtcggc caccgatggc cagctggcaa gccccggaga gaacggggaa gtccggccag
     2221 gccaggccaa gcgcttgggg ctggatgagt tcaacttcat caaggtgttg ggcaaaggca
     2281 gctttggcaa ggtcatgttg gcggaactca aaggcaaaga tgaagtctac gctgtgaagg
     2341 tcttgaagaa ggacgttatc ctacaagacg atgatgtgga ctgcacaatg acagagaaga
     2401 ggattttggc tctggctcgg aaacaccctt atctaaccca actctattgc tgcttccaga
     2461 ccaaggaccg cctcttcttc gtcatggaat atgtaaatgg tggagacctc atgttccaga
     2521 ttcagcggtc ccgaaaattt gatgagcctc gttctcggtt ctatgccgca gaggtcacat
     2581 cagccctcat gtttctccac cagcacggag tgatctacag ggatttgaaa ctggacaaca
     2641 tccttctaga tgcagaaggc cactgcaagc tggctgactt tgggatgtgc aaggaaggga
     2701 ttatgaatgg tgtgacaact accaccttct gtgggactcc tgactacata gctccagaga
     2761 tcctacagga gttggagtac ggcccctcag tggactggtg ggccctgggg gtgctgatgt
     2821 acgagatgat ggctgggcag cccccctttg aagctgacaa cgaggacgac ttgttcgaat
     2881 ccatccttca tgatgatgtt ctctatcctg tctggcttag caaggaagct gtcagcatcc
     2941 tgaaagcttt catgaccaag aacccgcaca agcgcctggg ctgtgtggca gcgcagaacg
     3001 gggaggacgc catcaagcaa catccattct tcaaggagat tgactgggta ctgctggagc
     3061 agaagaaaat caagcccccc ttcaagccga gaattaaaac caaaagagat gtcaataact
     3121 ttgaccaaga ctttacgcgg gaagagccaa tacttacact tgtggatgaa gcaatcatta
     3181 agcagatcaa ccaggaagaa ttcaaaggct tctcctactt tggtgaagac ctgatgccca
     3241 cgcgtacgcg gccgctcgag cagaaactca tctcagaaga ggatctggca gcaaatgata
     3301 tcctggatta caaggatgac gacgataagg tttaaacggc cggccgcggt catagctgtt
     3361 tcctgaacag atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     3421 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     3481 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     3541 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     3601 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     3661 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     3721 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     3781 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     3841 ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat
     3901 aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc
     3961 tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcggcgagc
     4021 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4081 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4141 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4201 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4261 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4321 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4381 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4441 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4501 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4561 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4621 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4681 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4741 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4801 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4861 ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc ctgccgcccc
     4921 gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga
     4981 agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc
     5041 ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct
     5101 ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag
     5161 cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     5221 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     5281 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     5341 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     5401 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     5461 cgggtagcca acgctatgtc ctgatagcga tccgccacac ccagccggcc acagtcgatg
     5521 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     5581 acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag ttcggctggc
     5641 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     5701 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     5761 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     5821 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     5881 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     5941 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     6001 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     6061 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     6121 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa
     6181 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     6241 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     6301 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     6361 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     6421 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     6481 tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt
     6541 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6601 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6661 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6721 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6781 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6841 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6901 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6961 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7021 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7081 aacgcgaatt tt
//
