LOCUS       V024775                 9568 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024775
VERSION     V024775
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             784..4227
                     /label="Prdm15(NM_144789)"
                     /note="Prdm15(NM_144789)"
                     /gene="Prdm15"
     CDS             4234..4260
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4264..4290
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4294..4320
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    4344..4932
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4998..5046
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5248..5676
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5690..6019
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6086..6880
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7056..7177
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7226..7242)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7250..7266
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7274..7304)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7319..7340
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7628..8216)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8387..9247)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9248..9352)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtg cccacctact
      721 atctgggaaa agggtggaca ggtgggggcc aggtggagcc tgcgtgctcc tgaggtatca
      781 gcaatggctg aagatggtag tgaggagatc atgtttatct ggtgtgaaga ctgcagccag
      841 taccatgact cggagtgtcc tgaactggga cctgtggtca tggtcaagga ctcctttgtg
      901 ctgagcaggg caaggtcctc cctcccctct aacctggaga tcaggcgcct ggatgacggg
      961 gctgaaggcg tgtttgcagt gacccagcta gtcaagcgca cacaatttgg tccgtttgag
     1021 tccaggagag tcgccaagtg ggagaaagag tcggcatttc ctctgaaggt atttcagaag
     1081 gacgggcatc ctgtgtgctt cgacacttcc aatgaggatg actgcaactg gatgatgcta
     1141 gtgcgaccag cactggagcc tgggcaccag aacctgacag cctaccaaca tggcagtgac
     1201 gtgtatttca ccacctcaaa ggacattcct gcaggcactg aactgcgtgt gtggtatgct
     1261 gccttttatg ccaagaagat ggacaagccc atgctaaagc aggcctgctc cagtgttcag
     1321 gctgcaggca cccctgagcc cagtgtctct gtggaacctg agcgtggcca atgggtgtgt
     1381 aaggtgtgct ccaacacctt cctggagctt cagctcctga acgagcatct cttgggccat
     1441 ctggagcaag ccaaaagcct ccctgcaggt ggccagcagc atgaggcagc ttctgagaag
     1501 gagcctgatg cacccaggat ggagcctccc acagcagccg agagcaagag catccagagt
     1561 gtcatggtca ccaaagagcc aaagaaaaag cctcgaagag ggaggaaacc caaagcatcc
     1621 aaggttgagc agcctctggt cattatcaaa gacaaggagc cttcagagca tgtggcagag
     1681 atcataactg agatcccccc agatgagcct gtgagtgcaa caccagatga acggatcatg
     1741 gagttagtct tggggaagct ggccgccccc acaaatgaag ccagctcagt gccaaagttc
     1801 ccacatcatc caagcagcac cattgccctc aagaggggct tggttctgtc cagcagacat
     1861 ggcgtccggc ggaagctcgt ccgtcagctg ggtgagcata aacgcatcca tcagtgtggc
     1921 acctgcagca aggtcttcca gaacagcagc aacctgagcc ggcacgtgcg ctcgcacggt
     1981 gagtgcgcgc acggggacaa gctgtttaaa tgtgaggaat gttcgaaact gttcagccgt
     2041 aaggaaagtc taaagcagca tgtttcctac aagcacagcc ggaatgaggt ggacggtgag
     2101 taccgctacc gctgtggcag ctgtgggaag acattccgaa tggagagtgc actggagttc
     2161 cacaactgta ggacagatga caagacgttc caatgtgaga tgtgtttcag attcttctcc
     2221 accaacagca acctctctaa gcacaagaag aaacatgggg acaagaagtt tgcctgtgag
     2281 gtctgcagta agatgttcta ccgcaaggat gttatgctgg accaccagag gcgacacttg
     2341 gatggtgtgc ggcgggtgaa gagagaagac ttggaggcca gcggggaaag cctggttcgt
     2401 tacaagaagg aaccttctgg atgcccagtg tgtggcaagg tgttctcctg tcggagcaac
     2461 atgaacaagc acctgctgac ccacggtgac aagaagtaca cctgcgagat ctgtgggcgc
     2521 aagttcttcc gagtggacgt actcagggac cacatccatg tccacttcaa ggacatcgcg
     2581 ctaatggatg accaccagcg ggaggagttc attggcaaaa ttgggatctc ctcagaggag
     2641 aatgatgaca actctgatga gagcgcagac tctgagccac acaagtacag ctgcaagagg
     2701 tgccagctca ctttcggccg tgggaaggaa tacctgaagc acatcatgga ggtgcacaag
     2761 gagaagggcc acggctgcag catctgccac cgacgcttcg cactcaaggc cacctaccat
     2821 gcccacatgg tcatccaccg ggagaacctg cctgacccca acgtgcagaa gtacattcac
     2881 ccctgtgaga tctgtgggag gatattcaac agcattggga acttggagcg ccacaaactc
     2941 atccacacag gtgtgaaaag ccacgcctgt gagcagtgtg ggaagtcctt tgccaggaag
     3001 gacatgctga aagagcacat gcgtgtgcat gacaacatcc gggagtacct gtgcgccgag
     3061 tgtgggaaag gtatgaagac caagcatgca ctgcgccacc atatgaagct gcacaaaggc
     3121 atcaaggagt atgagtgcaa ggaatgtcac cgcaagtttg cacagaaggt caacatgctc
     3181 aagcactaca aacggcacac aggaattaaa gacttcatgt gtgaattgtg tgggaagaca
     3241 ttcagtgaga ggaacacaat ggagacacac aagctcatcc acacagtagg caagcagtgg
     3301 acttgttccg tgtgtgacaa gaagtacgtc acagagtaca tgcttcagaa acacgtccag
     3361 ctcactcatg acaaggtgga agcacagagt tgccaactat gtggcaccaa agtgtcaacc
     3421 cgggcctcca tgagcagaca tatgcgacgc aagcaccccg aggtcctcgc tgttcggatt
     3481 gacgatctgg accacctccc cgagaccacc accatcgatg catcttccat cggcattgtc
     3541 cagcctgcgc tgggcctgga gcaggaagaa ctggcagaag gtaagcatgg aaaggctgcc
     3601 aagcgcagcc acaagagaaa gcagaagcca gaggaagagg caggggctcc agtgcctgag
     3661 gataccacct tcagtgaata ccccgaaaag gagccagagt tcacaggcag tgtgggtgac
     3721 gagacaaatt ctgctgtaca gagtatccaa caggtggtgg tgaccctagg ggaccccaat
     3781 gtgactgctc catcaagctc agttggtcta accaacatca ccgtgacccc catcaccact
     3841 gcagctggaa ctcagtttac caatttacag ccagtggctg ttggacatct taccaaccct
     3901 gatcgccagc tacagctaga taactccatc ctgactgtga cctttgatac tgtcagtggc
     3961 tccgccatgt tgcacaaccg gcaaaatgat gtccagatcc acccacagcc agaagccaca
     4021 aaccctcagt cagtggccca ctttatcaac cttaccacac tggtaaactc catcacaccc
     4081 ttggggaacc aactcagtga gcagcaccca ctcacctggc gagcagtgcc ccagacagat
     4141 gtgctgcagc cgccacaggc ccctgcagcc ccgcagcagg cggtgcaacc ccaggtacag
     4201 aatgagcagc agcagatgta cagctacctc gagtacccat acgacgtacc tgattatgca
     4261 acttatcctt atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca
     4321 tagtgagggc ccgatatctc gacaatcaac ctctggatta caaaatttgt gaaagattga
     4381 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4441 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4501 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4561 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4621 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4681 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     4741 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4801 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4861 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4921 ccgcctcccc gcctggaaac gggggaggct aactgaaaca cggaaggaga caataccgga
     4981 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt
     5041 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     5101 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     5161 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcagatctgc
     5221 gcagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     5281 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5341 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5401 ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5461 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5521 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5581 ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa
     5641 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     5701 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5761 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5821 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     5881 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5941 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     6001 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     6061 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     6121 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     6181 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     6241 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     6301 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     6361 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     6421 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6481 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6541 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6601 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6661 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6721 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6781 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     6841 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgcg
     6901 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     6961 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     7021 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     7081 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7141 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     7201 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7261 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7321 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7381 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7441 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7501 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7561 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7621 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7681 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7741 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7801 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     7861 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7921 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7981 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8041 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8101 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8161 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8221 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8281 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8341 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8401 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8461 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8521 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8581 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8641 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8701 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8761 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8821 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8881 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8941 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9001 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9061 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9121 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9181 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9241 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9301 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9361 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtcgac
     9421 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     9481 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     9541 cgacaattgc atgaagaatc tgcttagg
//
