LOCUS       V031043                 7350 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031043
VERSION     V031043
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3497
                     /label="Prdm1(NM_007548)"
                     /note="Prdm1(NM_007548)"
                     /gene="Prdm1"
     CDS             3516..3545
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3564..3587
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3607..3623)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3634..4256
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4405..4993)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5322..5369
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5601..6395)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6430..6787)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6789..6893)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6920..7348
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gttggatctt ctcttggaaa aacgtgtggg tacgaccttg gctgccccca
     1081 agtctagctc cggctccgtg aagtttcaag gactggcaga gactgggatc atgaaaatgg
     1141 acatggagga cgctgatatg actttgtgga cagaggccga gtttgaagag aagtgtacat
     1201 acatagtgaa cgaccacccc tgggattccg gcgctgacgg gggtacttct gttcaagccg
     1261 aggcatcctt accaaggaac ctgcttttca agtatgctgc caacaacagc aaagaggtta
     1321 ttggcgtggt aagtaaggag tacataccga agggaacacg ctttggaccc ctcatcggtg
     1381 aagtctacac taatgacaca gttcccaaga atgccaacag gaagtatttt tggcggatct
     1441 attccagaga ggagttccac cacttcattg atggctttaa tgaggagaaa agcaactgga
     1501 tgcgctacgt gaatccagct cactctgccc gggagcaaaa cctggctgcc tgtcagaacg
     1561 ggatgaacat ctacttctac actattaagc ctatccctgc caaccaggaa cttcttgtgt
     1621 ggtattgtcg ggactttgcg gagaggctcc actaccctta tcctggagag ctcacagtga
     1681 taaatctcac acaaacggaa agcaacccaa agcaatacag tagtgagaaa aatgaactct
     1741 acccaaagag tgtccccaag agagagtaca gcgtgaaaga aattctaaaa ctggactcca
     1801 atccctccaa aaggaaggac atctaccgtt ccaacatttc acccttcact ttagaaaagg
     1861 acatggatgg ctttcggaaa aatgggagcc ccgacatgcc cttctaccct cgggtggttt
     1921 atcctatccg ggcacctctg ccagaagact ttttgaaagc gtccctggcc tatgggatgg
     1981 agagacccac ctacataact cacagtcccc ttccgtcttc cacaactcca agtccccctg
     2041 cgagcagcag cccggagcag agccttaaga gctccagccc ccacagcagc ccgggaaaca
     2101 cggtgtcacc cctggcgcca ggcctcccag aacaccggga ctcctactcc tacttgaatg
     2161 tttcctatgg ttccgagggc ctgggctcct accctggcta tgcacctgcc ccccacctcc
     2221 caccagcttt cattccttct tacaatgctc actaccccaa gttcctgttg ccaccgtacg
     2281 gcattagttc caatggcttg agcaccatga acaacatcaa tggtatcaac aacttcagcc
     2341 tcttccctag gttgtatccc gtctacagta acctccttag tggcagcagc ctgcctcatc
     2401 ccatgctcaa tccagcttcc ctaccgagtt ccctgcctac cgatggagcc cggaggctgc
     2461 ttccaccgga gcaccccaaa gaggtgctta tcccagcacc ccacagtgcc ttctccctta
     2521 ccggggctgc agccagcatg aaggacgaga gtagtccccc cagcggatct ccaacggcgg
     2581 gaactgcagc cacgtcagaa cacgtggtac aacccaaagc tacctcatca gtgatggcgg
     2641 cccccagcac tgacggagcc atgaatctca ttaaaaacaa acgaaacatg actggttaca
     2701 agactcttcc ttaccctctg aagaaacaga atggcaagat caagtatgag tgcaatgtct
     2761 gtgccaagac gttcggtcag ctctccaacc tgaaggtcca cctgagagtg cacagtggag
     2821 aacggccttt caagtgccag acctgcaaca agggttttac tcagctcgcc cacctgcaga
     2881 aacactactt ggtacacaca ggagagaagc cacatgagtg ccaggtctgc cacaagagat
     2941 ttagcagcac aagcaatctc aagacccacc ttcgattgca ttctggagaa aaaccttacc
     3001 aatgtaaggt gtgccctgcc aagtttacgc aatttgtgca cctgaagctg cacaagcgac
     3061 tgcatacccg ggagcggcct cacaagtgtg cccagtgtca caagagctac atccatctct
     3121 gcagcctcaa ggtccacctg aagggcaact gccctgcggg cccagctgct gggctgcctt
     3181 tggaggatct gacccgaatc aatgaagaaa ttgagaggtt cgacatcagc gacaatgcag
     3241 accgtcttga ggacatggag gacagtgtcg atgtgacctc catggtggag aaggagattc
     3301 tagctgtggt cagaaaagag aaagaagaaa ccagtctgaa agtgtctttg caaagaaaca
     3361 tggggaacgg cctcctctcc tcagggtgca gcctctatga gtcatcggac ctgtccctca
     3421 tgaagttgcc tcacagcaac ccactacctc tggtgcctgt aaaggtcaaa caagaaacag
     3481 ttgaaccgat ggatcctacg cgtacgcggc cgctcgagca gaaactcatc tcagaagagg
     3541 atctggcagc aaatgatatc ctggattaca aggatgacga cgataaggtt taaacggccg
     3601 gccgcggtca tagctgtttc ctgaacagat cccgggtggc atccctgtga cccctcccca
     3661 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     3721 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     3781 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     3841 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     3901 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     3961 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     4021 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     4081 gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag
     4141 gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc
     4201 ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt
     4261 acgcggccag cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4321 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4381 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4441 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4501 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4561 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4621 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4681 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4741 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4801 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4861 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4921 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4981 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5041 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5101 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat
     5161 ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     5221 gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt
     5281 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     5341 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
     5401 cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc
     5461 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     5521 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     5581 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     5641 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     5701 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcgatc cgccacaccc
     5761 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     5821 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg
     5881 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     5941 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     6001 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     6061 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     6121 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     6181 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     6241 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     6301 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     6361 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     6421 tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     6481 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     6541 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     6601 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     6661 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     6721 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc
     6781 gcctcaggac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6841 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6901 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6961 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7021 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7081 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7141 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7201 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7261 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7321 ctgatttaac aaaaatttaa cgcgaatttt
//
