LOCUS V046779 7621 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046779 VERSION V046779 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..2280 /label="Ppp3cb(NM_008914)" /note="Ppp3cb(NM_008914)" /gene="Ppp3cb" CDS 2287..2313 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2317..2343 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2347..2373 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 2397..2985 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3051..3099 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3301..3729 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3743..4072 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4139..4933 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5109..5230 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5279..5295) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5303..5319 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5327..5357) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5372..5393 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5681..6269) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6440..7300) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7301..7405) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc cgccccggag 721 ccggcccggg ccgcgccgcc cccgcccccg cccccaccgc cccctctcgg ggccgaccgc 781 gtcgtcaaag ctgttccttt tcccccaact catcggctga catctgaaga agtgtttgat 841 atggatggga tacccagggt tgatgttctg aagaaccact tggtaaaaga agggcgggtg 901 gatgaagaaa ttgcactaag aattatcaat gagggtgctg ccatacttcg gcgggagaaa 961 accatgatag aagtagaagc tccaattaca gtgtgtggtg acatccatgg ccaatttttt 1021 gatctgatga aactttttga agtaggagga tcacctgcta atacacgata cctttttctt 1081 ggtgattatg tggacagagg ttattttagt atagagtgtg tcttatattt atgggtcttg 1141 aagattctat acccaagcac attattcctt ctgagaggca accatgaatg cagacacctt 1201 actgaatatt ttacctttaa gcaggaatgt aaaattaaat attcagaaag agtctatgaa 1261 gcttgtatgg aggcttttga cagcttgccc cttgctgcac ttctaaacca acaatttctt 1321 tgtgttcatg gtggactttc accagaaata cacacactgg atgatattag gagattagat 1381 agatttaaag agccacctgc atttggacca atgtgtgact tgctatggtc tgatccttct 1441 gaagactttg gaaatgaaaa atcacaagaa cattttagtc ataatacagt tcgaggatgt 1501 tcttattttt ataactatcc agcagtgtgt gaatttttgc aaaacaataa tttgttatcg 1561 attattagag ctcatgaagc tcaagatgca ggctatagaa tgtacagaaa aagtcaaact 1621 acagggtttc cttcattaat aacaattttt tcggcaccta attacttaga tgtctacaat 1681 aataaagctg ctgtactaaa gtatgaaaat aatgtgatga acattcgaca gtttaattgc 1741 tctccacatc cttattggtt gcccaatttt atggatgtct ttacatggtc tttaccattt 1801 gttggagaaa aagtgacaga aatgttggta aatgttctga gtatttgttc tgatgatgaa 1861 ctaatgacag aaggtgaaga ccagtttgat gtaggttcag ctgcagcccg gaaagaaatc 1921 ataagaaaca agatccgagc aattggcaag atggcaagag tcttctctgt tctcagggag 1981 gagagtgaaa gcgtgctgac actcaagggc ctgactccca cagggatgtt gcctagtgga 2041 gtgttggctg gaggacggca gaccttgcaa agtgccacag ttgaggctat tgaggctgaa 2101 aaagcaatac gaggattctc tccaccacat agaatctgca gttttgaaga ggcaaagggt 2161 ttggatagga tcaatgagag aatgccaccc cggaaagatg ctgtgcagca agatggtttc 2221 aattccctga acaccgcaca taccactgag aaccacggga ctggcaacca tagtgcccag 2281 ctcgagtacc catacgacgt acctgattat gcaacttatc cttatgatgt acctgattat 2341 gctagctatc cttatgatgt cccagactac gcatagtgag ggcccgatat ctcgacaatc 2401 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 2461 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 2521 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 2581 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 2641 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 2701 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 2761 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 2821 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 2881 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 2941 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga aacgggggag 3001 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa 3061 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc 3121 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt 3181 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg 3241 tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc 3301 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 3361 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 3421 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta 3481 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 3541 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 3601 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 3661 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 3721 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 3781 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 3841 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 3901 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 3961 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 4021 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 4081 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 4141 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 4201 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 4261 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 4321 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 4381 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 4441 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 4501 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 4561 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 4621 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 4681 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 4741 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 4801 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 4861 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 4921 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc 4981 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 5041 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 5101 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 5161 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 5221 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 5281 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 5341 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 5401 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 5461 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 5521 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 5581 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 5641 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 5701 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 5761 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 5821 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct 5881 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 5941 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 6001 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 6061 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 6121 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 6181 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 6241 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 6301 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 6361 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 6421 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 6481 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 6541 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 6601 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 6661 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 6721 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 6781 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 6841 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 6901 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 6961 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 7021 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 7081 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 7141 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 7201 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 7261 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 7321 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 7381 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 7441 gatcgggaga tctcccgatc ccctatggtc gactctcagt acaatctgct ctgatgccgc 7501 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag 7561 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag 7621 g //