LOCUS       V029799                 7923 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029799
VERSION     V029799
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..4070
                     /label="Ppargc1b(NM_133249)"
                     /note="Ppargc1b(NM_133249)"
                     /gene="Ppargc1b"
     CDS             4089..4118
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             4137..4160
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(4180..4196)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    4207..4829
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4978..5566)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5895..5942
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(6174..6968)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(7003..7360)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(7362..7466)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7493..7921
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcggggaac gactgcggcg cgctgctgga tgaagagctc tcgtccttct
     1081 tcctcaacta tctctctgac acgcagggtg gggactctgg agaggaacag ctgtgtgctg
     1141 acttgccaga gcttgacctc tcccagctgg acgccagtga ctttgactca gccacgtgct
     1201 ttggggagct gcagtggtgc ccggagacct cagagacaga gcccagccag tacagccccg
     1261 atgactccga gctcttccag attgacagtg agaatgaagc tctcttggct gcgcttacga
     1321 agaccctgga tgacatcccc gaagacgatg tggggctggc tgccttccca gaactggatg
     1381 aaggcgacac accatcctgc accccagcct cacctgcccc cttatctgca ccccccagcc
     1441 ccaccctgga gaggcttctg tccccagcgt ctgacgtgga cgagctttca ctgctacaga
     1501 agctcctcct ggccacatcc tccccaacag caagctctga cgctctgaag gacggggcca
     1561 cctggtccca gaccagcctc agttccagaa gtcagcggcc ttgtgtcaag gtggatggca
     1621 cccaggataa gaagaccccc acactgcggg ctcagagccg gccttgtacg gaactgcata
     1681 agcacctcac ttcggtgctg ccctgtccca gagtgaaagc ctgctcccca actccgcacc
     1741 cgagccctcg gctcctctcc aaagaggagg aggaggaggt gggggaggat tgcccaagcc
     1801 cttggccgac tccagcctcg ccccaagact ccctagcaca ggacacggcc agccccgaca
     1861 gtgcccagcc tcccgaggag gatgtgaggg ccatggtaca gctcattcgc tacatgcata
     1921 cctactgcct gcctcagagg aagctgcccc aacgggcccc agagccaatc ccccaggcct
     1981 gcagcagcct ctccaggcag gttcaacccc gatcccggca tccccccaaa gccttctgga
     2041 ctgagttctc tatcctaagg gaacttctgg cccaagatat cctctgtgat gttagcaagc
     2101 cctaccgcct ggccatacct gtctatgctt ccctcacacc tcagtccagg cccaggcccc
     2161 ccaaggacag tcaggcctcc cctgcccact ctgccatggc agaagaggtg agaatcactg
     2221 cttcccccaa gagcaccggg cctagaccca gcctgcgtcc tctgaggctg gaggtgaaac
     2281 gggatgttaa caagcctaca aggcaaaagc gggaggaaga tgaggaggag gaggaggaag
     2341 aagaagaaga ggaagaagaa aaagaagagg aagaagagga gtggggcagg aagagaccag
     2401 gtcgtggcct gccatggacc aaactaggga ggaagatgga cagctccgtg tgccccgtgc
     2461 ggcgctccag gagactgaat ccagagctgg gtccctggct gacattcact gatgagccct
     2521 taggtgctct gccctcgatg tgcctggata cagagaccca caacctggag gaagacctgg
     2581 gcagcctcac agacagtagt caaggccggc agctccccca gggatcccag atccccgccc
     2641 tggaaagccc ctgtgagagt gggtgcggag acacagatga agatccaagc tgcccacagc
     2701 ccacttccag agactcctcc aggtgcctca tgctggcctt gtcacaaagc gactctcttg
     2761 gcaagaagag ctttgaggag tccctgacgg tggagctttg cggcacggca ggactcacgc
     2821 cacccaccac acctccatac aagccaatgg aggaggaccc cttcaagcca gacaccaagc
     2881 tcagcccagg ccaagacaca gctcccagcc ttccctcccc cgaggctctt ccgctcacag
     2941 ccaccccagg agcttcccac aagctgccca agaggcaccc agagcgaagc gagctcctgt
     3001 cccatttgca gcatgccaca acccaaccag tctcacaggc tggccagaag cgccccttct
     3061 cctgctcctt tggagaccac gactactgcc aggtgctcag gccagaggct gccctgcaga
     3121 ggaaggtgct gcggtcctgg gagccaatcg gggtccacct tgaagacttg gcccagcagg
     3181 gtgcccctct gccaacggaa acaaaggccc ctaggaggga ggcaaaccag aactgtgacc
     3241 ctacccacaa ggacagcatg cagctaagag accatgagat ccgtgccagt ctcacaaagc
     3301 actttgggct gctggagact gctctggaag gtgaagacct ggcgtcctgt aaaagcccgg
     3361 agtatgacac cgtatttgag gacagcagca gcagcagtgg cgagagtagc ttcctgcttg
     3421 aggaggagga ggaagaggag gagggagggg aagaggacga tgaaggagag gactcagggg
     3481 tcagccctcc ctgctctgat cactgcccct accagagccc acccagtaag gccagtcggc
     3541 agctctgctc ccgaagccgc tccagttccg gctcctcgtc ctgcagctcc tggtcaccag
     3601 ccacccggaa gaacttcaga cgtgagagca gagggccctg ttcagatgga accccaagcg
     3661 tccggcatgc caggaagcgg cgggaaaagg ccatcggtga aggccgtgtg gtatacattc
     3721 gaaatctctc cagtgacatg agctctcggg aactaaagaa gcgctttgag gtgttcggtg
     3781 agattgtaga gtgccaggtg ctgacgagaa gtaaaagagg ccagaagcac ggttttatca
     3841 ccttccggtg ttcagagcac gctgccctgt ccgtgaggaa cggcgccacc ctgagaaagc
     3901 gcaatgagcc ctccttccac ctgagctatg gagggctccg gcacttccgt tggcccagat
     3961 acactgacta tgatcccaca tctgaggagt cccttccctc atctgggaaa agcaagtacg
     4021 aagccatgga ttttgacagc ttactgaaag aggcccagca gagcctgcat acgcgtacgc
     4081 ggccgctcga gcagaaactc atctcagaag aggatctggc agcaaatgat atcctggatt
     4141 acaaggatga cgacgataag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca
     4201 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
     4261 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     4321 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
     4381 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
     4441 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
     4501 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
     4561 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
     4621 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     4681 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct
     4741 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt
     4801 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcggcgag cggtatcagc
     4861 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5041 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5401 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5581 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5641 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5701 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc
     5761 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg
     5821 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg
     5881 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc
     5941 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct
     6001 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
     6061 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     6121 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
     6181 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     6241 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     6301 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa
     6361 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     6421 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     6481 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     6541 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     6601 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     6661 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     6721 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     6781 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     6841 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     6901 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     6961 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc
     7021 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     7081 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     7141 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     7201 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     7261 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     7321 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat
     7381 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7441 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     7501 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7561 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7621 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7681 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7741 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7801 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7861 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7921 ttt
//
