LOCUS       V044379                12432 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044379
VERSION     V044379
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..7112
                     /label="Plxnb1(NM_172775)"
                     /note="Plxnb1(NM_172775)"
                     /gene="Plxnb1"
     CDS             7119..7184
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    7208..7796
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7862..7910
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8112..8540
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8554..8883
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8950..9744
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9920..10041
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10090..10106)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10114..10130
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10138..10168)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10183..10204
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10492..11080)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11251..12111)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12112..12216)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgtc tgtcctcggc ccagttcttc
      781 tccaggtgtt ctgggccggg tgtgtcgtca ccctgcggtc ccctctgcca gctgctttca
      841 ctgccaatgg cacacatcta caacacttgg caagggaccc caccacaggt accctctatg
      901 taggggccac caacttcctg ttccagttga gccctgggct gcagctggaa gccgtggtgt
      961 ccacgggccc tgtgaatgac agccgggatt gcctgccacc tgtgatacct gatgaatgtc
     1021 cccaagccca gcctactaac aaccctaacc agctgctcct ggtgagccca gaggctctgg
     1081 tggtgtgtgg gagcgtacac cagggcatct gtgagctacg gagcctggga cagatcaggc
     1141 agctgctgct acggccagag cgacctgggg acacccagta tgtggctgca aatgaccctg
     1201 cagtcagtac agtggggctg gtggcccagg gattggtagg ggagcccctc ctgtttgtgg
     1261 ggcgggggta caccagcagg ggtgtaggtg gtgggattcc tcccattaca acccgagccc
     1321 tgcgaccacc ggacccccaa gctgccttct cttatgaaga aacagccaag ttagcagtgg
     1381 gccgcctgtc cgagtacagc caccacttcg tgagtgcctt tgtacgcggg gccagtgcat
     1441 acttcctgtt cttgcggcga gacctgaagg ccccttctag agctttccgt gcctatgtgt
     1501 ctcgagtgtg ccttcaggac cagcactact actcttatgt ggaattgccc ctggcctgcc
     1561 agggtggtcg ttacggtctt atccaggctg cagctgtagc cacgtccaag gaggtggccc
     1621 gtggggacgt actctttgca gctttctcct cagtggctcc tcccactgtg gattggcccc
     1681 tgtcagcatc tactggggca tctggaacct ctgtgctctg tgccttcccc ctggatgagg
     1741 tagaccagct tgctaattac actcgagatg cctgttatac tcgggaaggc cgtgctgaga
     1801 acgggaccaa ggttgctgac attgcatacg atgtcctttc cgactgtgcg cagctaccag
     1861 tggacacccc ggatgctttt ccatgtggct ctgaccacac acccagtccc atggtcagct
     1921 gtgtcccttt ggaagccacg ccaattctgg agctaccagg ggttcagcta acagctgtgg
     1981 ctgtcaccat ggaggatgga cacactattg ctttcctggg tgacagtcaa ggacagttgc
     2041 atagggtcta cttaggccct ggaagaagtg ctgccccata ttctaaacag agcatccagc
     2101 cggggtctcc tgtgaacaga gatcttacct ttgatggtac ctttgagcat ctctatgtag
     2161 caactcagac tactcttgtg aaggttcctg tggctccttg tgctcagcat ctggactgtg
     2221 actcttgcct tgctcacagg gacccttatt gcggatggtg tgtgctcctg ggcaggtgta
     2281 gtcgccggtc ggagtgctca agggaccagg gcccagagca gtggctgtgg agcttccagc
     2341 cggaactggg ctgtcttcga gtggtggccg tgagccctgc caatatcagt cgggaagaga
     2401 ggagggaggt tttcttgtca gtgccaggcc tgccatctct ctggccaggg gagtcatatt
     2461 tctgctactt tggagaccaa cagagtcctg ctctactgac cagttctggt gtgatgtgtc
     2521 cctccccaga ccccagtgag gctccagtgc tgcagagagg agccgaccat atctctgtga
     2581 acgtggagct caggtttggt gccgtggtga tcgccagcac ctccctctcc ttctatgact
     2641 gcgtggcagt tactgcgtct tccccatctg caccgtgccg ggcctgtgtg agcagccgct
     2701 ggggctgtaa ctggtgtgtg tggcagcagc tgtgcacaca caaggcctcg tgtgacgctg
     2761 ggcctatggt ggcaagccaa cagagcccac tccttcccct aatccctcct gcaagggatg
     2821 aactcacccc tttcccaccc acagtccccc aaaccacggt cactcctacc cccaacagct
     2881 tcccaataga gcctagggct ccctccacag cctcagatgt cctacctggg gccaagcctt
     2941 cccggctcag cctctggggc ccatgggcag gtcctggccc catactttcc cctacttcca
     3001 cagagtcacc tcttcatgag aagccccttc ctcctgaccc ccctaccata cctggaacca
     3061 ctgtccctgc ccccactggc ttgggaccat cgaccacacc tgaggacctc ttggcctcct
     3121 acccattccc ctcagatgca gctgcagtgt cccctgcaga gcctggccct gaggctctgc
     3181 cttccatggt ggctctggac cagccccctg gcactgttcc agacactact ttcccagggg
     3241 cccctggctc catgaagccc gttctggatt ggctcaccaa aggaggcggc gagctgcccg
     3301 aggcggatga gtggatgggg ggtgacacgc ccgccttctc cacttccaca ctcctctcag
     3361 gtgatggaga ctcagcagag cacgagggcc ctcctgcccc cctcatcctc ctgtccagcc
     3421 tcgactacca gtacgacacc cccgggctct gggagctggg agaggtgaat cagagggtga
     3481 gctcctgccc ctgtgtggag accgtccagg gctccttgct gataccggtc catgtggaac
     3541 gcgaagtcca gcttcgaggc aggaacctgt ggcttttcca ggatggcccg aggagcagcg
     3601 agtgtgtgct ggagctaggg agtcgggagg tggctgtgga ggctcaggtg gagtgtgcgc
     3661 cgcctccaga tgtctggtgc cacatcaagt gccagcagca tcagttcagc tatgaagctt
     3721 tgaagccaga actgcaggtg gggctgttcc tgcgttgggc aggcggtctg cgcgtggaca
     3781 gtgccgatgg gctgcatgtg gtgttgtatg actgctctgt gggacatggg gactgcagcc
     3841 gctgccaaac tgccatgcct cagtacgact gtgtgtggtg tgagggggag cgtccgcgtt
     3901 gtgtggcccg ggaagcctgt aatgaagccg agactgtggc cactcagtgc cccgcacctc
     3961 tcattcactc ggtggatcca ctgactggac ctatagatgg aggcacccgt gtcactatca
     4021 ggggctccaa cctgggccaa catgtgcagg atgtcctgga catggtcaga gtggccggag
     4081 ttccctgcgc tgtggatgct ggggagtatg atgtctctag tagtcttgtg tgcatcactg
     4141 gagccagcgg ggaggaggtg actggcactg tggcagtgga ggtgcctgga agaggacacg
     4201 gtgtctcaga gttcagcttt gcctatcagg atccaaaagt acactccatc ttcccagccc
     4261 gtggccctag agctggaggt acccgcctta ccctgcatgg ttctaagctc ctgactggac
     4321 ggctagagga catccgtgtg gtggttggag accagccttg ccacctgctc ctggagcagc
     4381 agtctgagca gctacactgt gagaccggcc cataccctgt gcctgctgaa cttccagtga
     4441 ctgtcttgtt tggggccact gagcggaggc ttcagcacgg ccaattcaag tatacatcag
     4501 accccaatgt cacctcagtg ggcccctcca agagcttctt cagcggagga cgtgagatat
     4561 gggtccgcgg ccaggatctt gatgtggtac agaggccaag aatccgagtg accgtggtcc
     4621 caagacagca tggccagggg cttgcacaga agcaacacgt ggtccctgag aaatttgagg
     4681 agccgtgtct cgtgaactcc tcccacctcc tcatgtgccg cactcccgct ctccctggcc
     4741 caccctggga ctctggggtc caggtggagt ttatcctcga caacatggtc tttgactttg
     4801 ctgcactgag ccccacaccc ttctcctatg aggctgatcc caccctgcgt tccctgaacc
     4861 ccgaggatcc cagcacgccg ttccggcaca agccagggag tgtgttctct gtggaggggg
     4921 agaatctgga cctcgccatg tctaaagaag aggtggtggc catgataggg gacgggccct
     4981 gcgtggtaaa gacactgacc cggaaccacc tgtactgtga gccccctgtg gagcagcccc
     5041 tgccacatcc ccatgccctc cgagaggctc cagatgcttt gcctgagttc acggtacaga
     5101 tgggcaacct gcgcttctcc ttgggtcatg tgcagtacga tggcgagagc cccgtggctt
     5161 ttcctgtggc agcccaagtg ggcttgggag tgggcacgtc tctcctggct ctgggtgtca
     5221 tcatcattgt cctcatatac aggaggaaga gcaagcaggc cctgagggac tataagaaag
     5281 tgcagatcca gctggagaac ctggagagca gtgtacggga ccgctgtaag aaggagttta
     5341 cagacctcat gacggagatg acggatctca ccagtgacct ccttggcagc ggtatcccct
     5401 tccttgacta caaagtgtat gctgagaggg tcttcttccc tgggtaccgg gagtccccct
     5461 tgcacaggga cctcggtgtg cctgacagca ggcgacccac cgtggaacag ggcctggggc
     5521 agctctccaa cctgctaaac agcaagctct tccttaccaa gttcatccac acactggaga
     5581 gtcagcgcac cttctctgct cgggaccgtg cctacgtggc atctctgctc actgttgcac
     5641 ttcacgggaa gcttgaatac ttcacggaca tactgcggac tctgctcagt gacctggtag
     5701 ctcagtatgt tgccaagaac cccaagctga tgctgcgcag gacagagacc gtggtagaaa
     5761 agctgctcac caactggatg tccatctgcc tctacacctt tgtgagggac tctgtgggag
     5821 agcctctgta tatgctcttc agagggatta agcatcaagt ggacaagggt cccgtggaca
     5881 gtgtgactgg caaagccaaa tacactctga atgacaaccg cctgctcaga gaggatgtgg
     5941 agtaccgtcc cttgaccttg aatgctcttc tggctgtggg gcctggggca ggagaagccc
     6001 agtgtgtacc tgtgaaagtc ctggactgtg acaccatctc ccaggccaag gagaagatgc
     6061 tagaccagct ttacaaggga gtgcctcttg cccagcggcc cgactcttgc accttggatg
     6121 ttgaatggcg gtctggagtg gctgggcacc ttatcctttc tgatgaggac gtcacttccg
     6181 aactccaggg tctgtggagg cgtctgaata cactgcaaca ttacaaggtc ccagatggag
     6241 caacggtggc ccttgtcccc tgcctcacca agcatattct tagggaaaac caggattatg
     6301 tccctgggga acggacccca atgctggagg atgtagatga ggggggcatc cggccctggc
     6361 acctggtaaa gccgagtgat gaaccagagc ctcccaggcc gaggaggggc agccttcggg
     6421 gtggggagcg tgagcgagcc aaggctatcc ctgagatcta cctgacacgc ctgctatcca
     6481 tgaagggcac actgcagaag tttgtggatg acctgttcca ggtgattctc agcaccagcc
     6541 gccctgtgcc tctggctgtg aagtacttct ttgacttgct ggatgaacaa gctcagcagc
     6601 atggcatctc tgatcaggat actatccaca tctggaagac caacagcctg ccgctaaggt
     6661 tctggatcaa catcatcaag aacccacagt ttgtgttcga tgtgcagact tcggataaca
     6721 tggatgctgt gctcctggtc attgcacaga ccttcatgga tgcttgcacc ctggccgacc
     6781 acaagctggg ccgggattct cccatcaaca aacttctgta tgctcgagat attccccgtt
     6841 acaaacagat ggtggaaagg tactatgcag acatcagaca gactgtcccg gccagtgacc
     6901 aagagatgaa ctcagtcttg gcggagctgt cccggaactg ctctgccgac cttggggcgc
     6961 gagtggctct gcatgaactc tacaagtata tcaacaagta ctatgaccag atcatcactg
     7021 ccctggagga ggatggcact gcccagaaga tgcagctggg ctaccggctc cagcagatcg
     7081 ccgctgctgt ggaaaacaag gtcacggatc taggatccga ctacaaagac catgacggtg
     7141 attataaaga tcatgacatc gactacaagg atgacgatga caagtagtga gggcccgata
     7201 tctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7261 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7321 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7381 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7441 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7501 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7561 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     7621 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7681 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7741 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7801 aaacggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     7861 acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg
     7921 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     7981 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     8041 cgcagccaac gtcggggcgg caggccctgc catagcagat ctgcgcagct ggggctctag
     8101 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     8161 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8221 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg
     8281 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8341 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8401 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8461 ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta
     8521 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     8581 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     8641 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8701 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8761 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8821 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     8881 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     8941 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     9001 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     9061 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     9121 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     9181 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     9241 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     9301 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     9361 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     9421 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     9481 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     9541 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     9601 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     9661 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     9721 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc
     9781 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     9841 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     9901 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     9961 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10021 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
    10081 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    10141 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    10201 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    10261 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    10321 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10381 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10441 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10501 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    10561 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    10621 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10681 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    10741 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    10801 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    10861 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    10921 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    10981 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    11041 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    11101 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    11161 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    11221 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    11281 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    11341 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    11401 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    11461 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    11521 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    11581 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    11641 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    11701 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    11761 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    11821 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    11881 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    11941 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    12001 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    12061 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    12121 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    12181 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    12241 tgacgtcgac ggatcgggag atctcccgat cccctatggt cgactctcag tacaatctgc
    12301 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
    12361 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
    12421 aatctgctta gg
//
