LOCUS       V031304                 7053 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031304
VERSION     V031304
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3200
                     /label="Pik3r1(NM_001077495)"
                     /note="Pik3r1(NM_001077495)"
                     /gene="Pik3r1"
     CDS             3219..3248
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3267..3290
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3310..3326)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3337..3959
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4108..4696)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5025..5072
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5304..6098)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6133..6490)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6492..6596)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6623..7051
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gagtgcagag ggctaccagt acagagcact gtacgactac aagaaggagc
     1081 gagaggaaga cattgaccta cacctggggg acatactgac tgtgaataaa ggctccttag
     1141 tggcacttgg attcagtgat ggccaggaag cccggcctga agatattggc tggttaaatg
     1201 gctacaatga aaccactggg gagaggggag actttccagg aacttacgtt gaatacattg
     1261 gaaggaaaag aatttcaccc cctactccca agcctcggcc ccctcgaccg cttcctgttg
     1321 ctccgggttc ttcaaaaact gaagctgaca cggagcagca agcgttgccc cttcctgacc
     1381 tggccgagca gtttgcccct cctgatgttg ccccgcctct ccttataaag ctcctggaag
     1441 ccattgagaa gaaaggactg gaatgttcga ctctatacag aacacaaagc tccagcaacc
     1501 ctgcagaatt acgacagctt cttgattgtg atgccgcgtc agtggacttg gagatgatcg
     1561 acgtacacgt cttagcagat gctttcaaac gctatctcgc cgacttacca aatcctgtca
     1621 ttcctgtagc tgtttacaat gagatgatgt ctttagccca agaactacag agccctgaag
     1681 actgcatcca gctgttgaag aagctcatta gattgcctaa tatacctcat cagtgttggc
     1741 ttacgcttca gtatttgctc aagcattttt tcaagctctc tcaagcctcc agcaaaaacc
     1801 ttttgaatgc aagagtcctc tctgagattt tcagccccgt gcttttcaga tttccagccg
     1861 ccagctctga taatactgaa cacctcataa aagcgataga gattttaatc tcaacggaat
     1921 ggaatgagag acagccagca ccagcactgc cccccaaacc acccaagccc actactgtag
     1981 ccaacaacag catgaacaac aatatgtcct tgcaggatgc tgaatggtac tggggagaca
     2041 tctcaaggga agaagtgaat gaaaaactcc gagacactgc tgatgggacc tttttggtac
     2101 gagacgcatc tactaaaatg cacggcgatt acactcttac actaaggaaa ggaggaaata
     2161 acaaattaat caaaatcttt caccgtgatg gaaaatatgg cttctctgat ccattaacct
     2221 tcaactctgt ggttgagtta ataaaccact accggaatga gtctttagct cagtacaacc
     2281 ccaagctgga tgtgaagttg ctctacccag tgtccaaata ccagcaggat caagttgtca
     2341 aagaagataa tattgaagct gtagggaaaa aattacatga atataatact caatttcaag
     2401 aaaaaagtcg ggaatatgat agattatatg aggagtacac ccgtacttcc caggaaatcc
     2461 aaatgaaaag aacggctatc gaagcattta atgaaaccat aaaaatattt gaagaacaat
     2521 gccaaaccca ggagcggtac agcaaagaat acatagagaa gtttaaacgc gaaggcaacg
     2581 agaaagaaat tcaaaggatt atgcataacc atgataagct gaagtcgcgt atcagtgaga
     2641 tcattgacag taggaggagg ttggaagaag acttgaagaa gcaggcagct gagtaccgag
     2701 agatcgacaa acgcatgaac agtattaagc cggacctcat ccagttgaga aagacaagag
     2761 accaatactt gatgtggctg acgcagaaag gtgtgcggca gaagaagctg aacgagtggc
     2821 tggggaatga aaataccgaa gatcaatact ccctggtaga agatgatgag gatttgcccc
     2881 accatgacga gaagacgtgg aatgtcggga gcagcaaccg aaacaaagcg gagaacctat
     2941 tgcgagggaa gcgagacggc actttccttg tccgggagag cagtaagcag ggctgctatg
     3001 cctgctccgt agtggtagac ggcgaagtca agcattgcgt cattaacaag actgccaccg
     3061 gctatggctt tgccgagccc tacaacctgt acagctccct gaaggagctg gtgctacatt
     3121 atcaacacac ctccctcgtg cagcacaatg actccctcaa tgtcacacta gcatacccag
     3181 tatatgcaca acagaggcga acgcgtacgc ggccgctcga gcagaaactc atctcagaag
     3241 aggatctggc agcaaatgat atcctggatt acaaggatga cgacgataag gtttaaacgg
     3301 ccggccgcgg tcatagctgt ttcctgaaca gatcccgggt ggcatccctg tgacccctcc
     3361 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     3421 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     3481 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     3541 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     3601 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     3661 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     3721 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     3781 caggcgtgaa ccactgctcc cttccctgtc cttctgattt taaaataact ataccagcag
     3841 gaggacgtcc agacacagca taggctacct ggccatgccc aaccggtggg acatttgagt
     3901 tgcttgcttg gcactgtcct ctcatgcgtt gggtccactc agtagatgcc tgttgaattg
     3961 ggtacgcggc cagcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4021 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4081 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4141 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4201 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4261 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4321 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4381 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4441 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4501 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     4561 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4621 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4681 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4741 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4801 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aacctgaggc
     4861 tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg
     4921 ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg
     4981 ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac
     5041 accgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
     5101 ctccttccgt gtttcagtta gcctccccct agggtgggcg aagaactcca gcatgagatc
     5161 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     5221 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
     5281 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     5341 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     5401 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg atccgccaca
     5461 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     5521 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc
     5581 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     5641 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     5701 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     5761 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     5821 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     5881 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac
     5941 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     6001 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     6061 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     6121 tcttgatcga tctttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
     6181 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     6241 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
     6301 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     6361 tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     6421 tgctggggag cctggggact ttccacaccc taactgacac acattccaca gctggttctt
     6481 tccgcctcag gactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     6541 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     6601 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6661 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6721 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6781 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6841 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6901 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6961 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7021 gagctgattt aacaaaaatt taacgcgaat ttt
//
