LOCUS V031148 8427 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031148 VERSION V031148 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..4574 /label="Phka1(NM_173021)" /note="Phka1(NM_173021)" /gene="Phka1" CDS 4593..4622 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 4641..4664 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(4684..4700) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 4711..5333 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(5482..6070) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 6399..6446 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(6678..7472) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(7507..7864) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(7866..7970) /gene="bla" /label="AmpR promoter" rep_origin 7997..8425 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gaggagccgc agtaactcgg gagtccgcct ggacggctat gctcggctgg 1081 tgcatcagac catcctgtgc catcagaatc cagtgacagg cttgcttcca gccagctatg 1141 atcaaaaaga tgcctgggtc cgagataatg tgtacagtat cctggctgtg tggggtttgg 1201 gcctggcata tcgcaaaaat gctgaccgtg atgaggacaa ggcaaaggcc tatgaactgg 1261 agcagagtgt agtgaagtta atgaggggac tgctgcactg catgatcaga caggtggata 1321 aagtagagtc cttcaagtac agtcagagta ctaaagatag cctccatgcc aagtacaaca 1381 ccaaaacttg tgccactgtg gtgggtgatg accagtgggg acacctgcag ttggatgcta 1441 cttctgtgta cctgctcttc ctagcacaaa tgactgcctc aggactccat atcatccaca 1501 gcctagacga agtcaatttt atacagaacc tcgtgtttta cattgaagct gcatataaaa 1561 ctgctgactt tgggatatgg gaacgtggcg ataagacaaa ccaaggcatc tcggaattga 1621 atgcgagttc agttggaatg gccaaggcag ccctggaagc actagatgaa ttagacttgt 1681 ttggtgtgaa aggtgggcca caatcagtta tccatgtcct ggctgatgaa gtccaacact 1741 gccagtctat cctgaattca ctactgccca gggcttcaac atccaaagaa gttgatgcca 1801 gtctgctctc agtggtctct ttcccagcct ttgctgtaga ggacagccat ttggtggagc 1861 tcaccaaaca ggagatcatc accaagcttc agggtcgtta tggttgctgt cgttttctgc 1921 gagatggata taaaactcct aaagaggatc ccaatcgcct atactatgaa ccagctgagc 1981 tgaagctatt tgaaaacatt gagtgcgaat ggccattgtt ctggacatac tttatccttg 2041 atgggatctt cagtggcaac gtagaacagg ttcaagaata tagagaggct cttgatgcag 2101 tcctcatcaa gggcaaaaat ggagtccctc ttcttccaga gctgtacagt gtccctcctg 2161 acagggttga tgaagagtat caaaatcccc acactgtgga tcgagtccct atgggaaaat 2221 tgcctcacat gtggggtcag tctctataca ttttaggaag cttgatggca gagggatttt 2281 tagctcctgg agaaattgat cccctgaatc gtaggttttc tactgtgcca aagccagatg 2341 tggtggttca agtctccatt ctggctgaaa cagaagaaat caaggccatt ttgaaggaca 2401 aaggaattga tgtggagacc attgctgaag tgtaccccat aagagtacag ccagctcgta 2461 ttctcagcca tatttattct agtctaggat gcaacagtag aatgaaactc agtggacgac 2521 cctacaggct catgggtgtg cttggaacat caaaacttta tgacattcgc aaaactatct 2581 ttactttcac tccacagttt atagaccagc aacagttcta cctggctctg gacaaccaga 2641 tgatagtaga aatgctcaga acagaccttt cctacctctg tagccgctgg aggatgacag 2701 gccagcccac gatcactttc cctatctcgc acaccatgct tgatgaagat ggaaccagct 2761 tgaattcaag tatcttggca gcactccgaa aaatgcagga tggctatttt ggtggggcca 2821 ggatccaaac aggtaaattg tcagagtttt tgacaacatc ttgctgcaca cacttaagct 2881 tcatggaccc tggacctgag ggtaagctgt acagtgaaga ttatgatgaa gactatgaag 2941 atgatttgga ctctggcaac tggatggaca gctatgattc aacaagtaat gatgttcaca 3001 tgtatcttcc tacaaagtta tttcagcctt ctcgtccttc actcaactta cttgactccc 3061 ctgagtctcc acaagatagc caggttcctt ccgttcatgt agaagtgcat cttcctaggg 3121 accagtctgg ggaagtggac ttccagtcat tggtttcaca gttgaaggag acctcaagct 3181 tacaggagca agctgatata ctctacatgc tgtattcaat gaaaggacct gactggaaca 3241 ctgaattgta tgaagaaggg ggggctactg tcagagagct tcttagtgaa ctgtatgtca 3301 aagttggtga aattcggcac tggggtctga tccgatatat ctctgggatc ttacggaaga 3361 aagtggaggc acttgatgag gcctgcacag accttctgtc ctaccagaaa cacctgacag 3421 taggacttcc tccagaacct cgagagaaga ccatctctgc gcctctaccg tacgaggcac 3481 tcactaagct gatagatgaa gccagtgaag gcgacatgag catctcaacc cttacacagg 3541 aaataatggt ctatcttgcc atgtatatga gaactcagcc tggcctcttt gcagaaatgt 3601 tcagacttcg aatcggtttg atcatacaag ttatggcaac agaactagca cactctcttc 3661 gatgttcagc tgaggaagcc acagagggcc tgatgaatct cagtccttca gccatgaaga 3721 acctcctgca tcacattctc agtggcaaag agtttggagt ggaacgaagc gttcgtccca 3781 ctgattcaaa tgtgagtcct gctatttcca tccatgagat tggtgctgtt ggagcaacaa 3841 aaactgaacg aactggaatc atgcagttaa aaagtgagat aaagcaggtg gaatttcgta 3901 ggctgtctgt ctcgatggag agtcagacta gtggtggtca tccctcgggt gtagatttga 3961 tgtcgccatc ctttctgtcc cctgcagcct gtattgctgc aagtagtgga tcctttccta 4021 cggtgtgtga tcatcagaca tctaaagata gtcgtcaagg ccagtggcaa cgcaggagaa 4081 ggctagatgg agcactaaat agagtaccaa ttggatttta tcaaaaagta tggaaaattt 4141 tgcagaaatg tcatgggctt tctgtggaag gttttgttct tccctcttca accactaggg 4201 agatgacccc aggtgagatt aaattctctg tccatgtgga gtctgtcctg aatcgtgtcc 4261 ctcagccaga ataccgccaa cttctggttg aagccatcct tgttctcacc atgctggcag 4321 atattgaaat tcatagcatt gggagcatca ttgctgtgga gaaaatagtt catattgcca 4381 acgacttgtt ccttcaagaa cagaaaaccc tcggcgcaga tgataccatg ttggcaaagg 4441 atcctgcatc tggcatctgt actctcctgt atgacagtgc acccagtggc agatttggca 4501 ccatgaccta cctctccaag gcagctgcca cctacgtgca ggagtttctg ccacacagcc 4561 tctgtgccat gcagacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc 4621 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc 4681 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg 4741 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 4801 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 4861 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 4921 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 4981 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 5041 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 5101 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 5161 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac 5221 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg 5281 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg 5341 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 5401 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 5461 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 5521 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 5581 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 5641 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 5701 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 5761 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 5821 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 5881 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 5941 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 6001 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 6061 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 6121 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 6181 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc 6241 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg 6301 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc 6361 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg 6421 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt 6481 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg 6541 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa 6601 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc 6661 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc 6721 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc 6781 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc 6841 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag 6901 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg 6961 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga 7021 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg 7081 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc 7141 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc 7201 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg 7261 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg 7321 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag 7381 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga 7441 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga 7501 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc 7561 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg 7621 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat 7681 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga 7741 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg 7801 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc 7861 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 7921 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 7981 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 8041 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 8101 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 8161 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 8221 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 8281 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 8341 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 8401 atttaacaaa aatttaacgc gaatttt //