LOCUS       V019375                 7910 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019375
VERSION     V019375
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1004..4072
                     /label="Phf8(NM_001113354)"
                     /note="Phf8(NM_001113354)"
                     /gene="Phf8"
     CDS             4076..4105
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             4124..4147
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(4167..4183)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    4194..4816
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4965..5553)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5882..5929
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(6161..6955)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6990..7347)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(7349..7453)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7480..7908
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccgcc accatggcct cggtgcctgt
     1021 gtattgcctc tgtcgactgc cttatgatgt gacccgcttc atgatcgagt gtgacatgtg
     1081 ccaggactgg tttcacggca gttgtgttgg tgttgaggag gagaaggctg ctgatattga
     1141 tctttaccac tgccctaact gtgaggtctt acatggacct tccattatga aaaaacgtcg
     1201 tggatcttcc aaaggacatg ataatcacaa ggggaagcca ctgaagactg gaagctctat
     1261 gtttatccga gaacttcggg gcagaacttt tgacagctca gatgaagtga ttctgaagcc
     1321 cactggaagt cagctgactg tggaattctt ggaagagaat agcttcagcg tgcctatcct
     1381 tgtcttgaag aaggatgggt tggggatgac attaccctct ccatcattca ctgtgaggga
     1441 tgtggaacac tatgttggtt ctgacaaaga gattgatgtg attgatgtgg cccgccaggc
     1501 tgactgcaag atgaaactcg gtgattttgt caaatactat tacagtggga agagggaaaa
     1561 agtcctcaat gtcattagtt tggaattctc cgataccagg ctttcaaacc tcgtggaaac
     1621 acccaggatt gttcgcaagc tgtcatgggt ggagaacttg tggccagagg aatgtgtctt
     1681 tgagagaccc aatgtgcaga agtactgcct catgagtgtg cgggatagct atacagattt
     1741 tcacattgac tttggtggga cctcagtttg gtaccatgtg cttaagggtg agaagatctt
     1801 ctacctgatc cgcccaacaa atgctaatct gactctcttt gagtgctgga gtagctcctc
     1861 caatcagaac gagatgttct ttggtgacca agtggaaaag tgctacaagt gttctgtgaa
     1921 gcaaggacaa acactgttta ttcctacagg atggatacat gctgtgttaa cacccgtgga
     1981 ctgcttagca ttcggaggga acttcttaca cagtcttaac attgaaatgc aactcaaggc
     2041 ttatgaaatt gagaagaggc tgagcacagc agaccttttc aagtttccca acttcgagac
     2101 catctgttgg tatgtgggaa aacatattct ggacatcttt cgaggcttac gagaaaatag
     2161 aagacaccct gcctcctacc tggtccatgg tggtaaagct ctgaacttgg catttagagc
     2221 ttggacaaag aaagaagctt tgccagacca cgaggatgag atcccagaga cagtgcggac
     2281 tgtacagctc attaaagatc tggctaggga gatccgtctg gttgaagaca tcttccaaca
     2341 gaacgttggg aagacgagca atatctttgg gctgcagagg atcttcccag ctggctccat
     2401 ccccttaacc aagccagccc attccacttc agtatccatg tccaagctgt cactgccctc
     2461 caaaaatggt tcaaagaaga aaggcctgaa gcccaaggat atcttcaaga aggcagagcg
     2521 aaagggcaag cagagttcag ccttggggcc tgctggccag ttgagctata atctcatgga
     2581 cccatacagt catcaggcac tgaagacagg cccttcccag aaagcaaagt tcaacatgag
     2641 tggtaccagc ttgaatgatt cagatgatga ctcagcagac atggaccttg atggcagtga
     2701 gaaccctctg gccctgttga tggctaatgg cagtacgaag aggatgaaga gtgtatccaa
     2761 atctcggaga gccaaaattg caaagaaggt agacagtgca agactggtag cagaacaggt
     2821 catgggagat gaatttgact tggattcaga tgatgagctg cagattgacg agagattggg
     2881 aaaggaaaag gcgaacctgt taataagatc aaaatttccc cggaagttgc cccgtgcaaa
     2941 accttgctct gaccccaacc gaattcgtga acctggagaa gttgagtttg acattgagga
     3001 ggactatacc acagatgagg acatggtgga aggggttgaa agcaagcttg ggaatgggag
     3061 tggagccggt ggaattcttg atctacttaa ggccagcagg caggtggggg gacctgacta
     3121 tgctgccctc actgaggccc cagcctcccc cagcactcaa gaggccatcc agggcatgtt
     3181 gtgtatggcc aacctgcagt cctcatcatc ctcaccagct acctccagtc tgcaggcttg
     3241 gtggactgga gggcaagaaa gaagcagcgg gagctccagc agtggcctgg gcactgtgtc
     3301 tagtagtcct gcttcccagc gcaccccagg gaagcggccc atcaagaggc cagcatactg
     3361 gaaaaacgag agtgaagagg aggagaatgc cagtcttgat gagcaagaca gcttgggagc
     3421 atgcttcaag gatgctgagt atatctatcc atctctggag tctgatgatg atgaccctgc
     3481 tttgaaatct cgacccaaga aaaagaagaa ttcagatgat gctccatgga gtcctaaagc
     3541 ccgtgtgact ccaaccctac ccaagcagga ccgtcctgtt cgtgagggga ccagagttgc
     3601 ctccattgag acaggcttgg ctgcagcagc tgcaaagctt gcccagcagg agctacaaaa
     3661 ggcccaaaag aagaaatata tcaagaagaa gcctttgctg aaggaggtag aacagccgcg
     3721 ccctcaagat tccaatccca tcatgacaat gcctgcacca actgtggcta caacacccca
     3781 gcctgacacc tcctcctcac cccagccacc tcctgagcct aaacaagagg ctctgtcagg
     3841 aagtcttgct gaccatgagt acactgctcg tcccaatgcc tttggcatgg ctcaggcaaa
     3901 tcgcagcacc acacccatgg cccctggagt cttcctcacc cagcggcgcc cttcagttgg
     3961 ttcccagagc agtcaggcag gacaaggaaa gcgtcctaaa aagggcctgg ccacagcaaa
     4021 gcagagactc ggccgcatcc tgaaaatcca cagaaatggc aagttacttc tgctcgagca
     4081 gaaactcatc tcagaagagg atctggcagc aaatgatatc ctggattaca aggatgacga
     4141 cgataaggtt taaacggccg gccgcggtca tagctgtttc ctgaacagat cccgggtggc
     4201 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     4261 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     4321 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
     4381 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
     4441 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat
     4501 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
     4561 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
     4621 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa
     4681 aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc catgcccaac
     4741 cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg tccactcagt
     4801 agatgcctgt tgaattgggt acgcggccag cggcgagcgg tatcagctca ctcaaaggcg
     4861 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4921 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4981 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5041 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5101 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5161 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5221 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5281 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5341 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5401 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5461 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5521 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5581 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     5641 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     5701 tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg
     5761 ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc
     5821 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta
     5881 tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
     5941 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag
     6001 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
     6061 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
     6121 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     6181 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     6241 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     6301 gatagcgatc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     6361 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     6421 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     6481 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     6541 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     6601 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     6661 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     6721 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt
     6781 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     6841 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     6901 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     6961 acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct
     7021 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     7081 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     7141 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
     7201 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
     7261 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     7321 ttccacagct ggttctttcc gcctcaggac tcttcctttt tcaatattat tgaagcattt
     7381 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7441 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     7501 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     7561 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7621 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     7681 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     7741 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     7801 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     7861 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
//
