LOCUS V018259 5771 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018259 VERSION V018259 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1010..1978 /label="PPP1CC(NM_002710)" /note="PPP1CC(NM_002710)" /gene="PPP1CC" CDS 1982..2011 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" primer_bind complement(2028..2044) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2055..2677 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2826..3414) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3743..3790 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4022..4816) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4851..5208) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5210..5314) /gene="bla" /label="AmpR promoter" rep_origin 5341..5769 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgccacca tggcggattt 1021 agataaactc aacatcgaca gcattatcca acggctgctg gaagtgagag ggtccaagcc 1081 tggtaagaat gtccagcttc aggagaatga aatcagagga ctgtgcttaa agtctcgtga 1141 aatctttctc agtcagccta tcctactaga acttgaagca ccactcaaaa tatgtggtga 1201 catccatgga caatactatg atttgctgcg actttttgag tacggtggtt tcccaccaga 1261 aagcaactac ctgtttcttg gggactatgt ggacagggga aagcagtcat tggagacgat 1321 ctgcctctta ctggcctaca aaataaaata tcctgagaat ttttttcttc tcagagggaa 1381 ccatgaatgt gccagcatca acagaattta tggattttat gatgaatgta aaagaagata 1441 caacattaaa ctatggaaaa ctttcacaga ctgttttaac tgtttaccga tagcagccat 1501 cgtggatgag aagatattct gctgtcatgg aggtttatca ccagatcttc aatctatgga 1561 gcagattcgg cgaattatgc gaccaactga tgtaccagat caaggtcttc tttgtgatct 1621 tttgtggtct gaccccgata aagatgtctt aggctggggt gaaaatgaca gaggagtgtc 1681 cttcacattt ggtgcagaag tggttgcaaa atttctccat aagcatgatt tggatcttat 1741 atgtagagcc catcaggtgg ttgaagatgg atatgaattt tttgcaaaga ggcagttggt 1801 cactctgttt tctgcgccca attattgcgg agagtttgac aatgcaggtg ccatgatgag 1861 tgtggatgaa acactaatgt gttcttttca gattttaaag cctgcagaga aaaagaagcc 1921 aaatgccacg agacctgtaa cgcctccaag gggtatgatc acaaagcaag caaagaaact 1981 cgagcagaaa ctcatctcag aagaggatct gtaaacggcc ggccgcggtc atagctgttt 2041 cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg 2101 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt 2161 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc 2221 aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag 2281 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc 2341 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt 2401 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg 2461 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct 2521 tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag acacagcata 2581 ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc actgtcctct 2641 catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca gcggcgagcg 2701 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 2761 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 2821 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 2881 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 2941 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 3001 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 3061 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 3121 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 3181 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 3241 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 3301 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 3361 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 3421 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 3481 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 3541 tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc tgccgccccg 3601 acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa 3661 gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga gtgccagccc 3721 tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt attctgtctt 3781 tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc 3841 ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag 3901 ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg 3961 aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg 4021 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga 4081 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac 4141 gggtagccaa cgctatgtcc tgatagcgat ccgccacacc cagccggcca cagtcgatga 4201 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca 4261 cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg 4321 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag 4381 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa 4441 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt 4501 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt 4561 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc 4621 gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa 4681 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt 4741 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc 4801 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag 4861 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc 4921 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg 4981 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg 5041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt 5101 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt 5161 ccacacccta actgacacac attccacagc tggttctttc cgcctcagga ctcttccttt 5221 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 5281 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 5341 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 5401 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 5461 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 5521 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 5581 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 5641 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 5701 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 5761 acgcgaattt t //