LOCUS       V043660                11821 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043660
VERSION     V043660
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..6480
                     /label="PLXND1(NM_015103)"
                     /note="PLXND1(NM_015103)"
                     /gene="PLXND1"
     CDS             6487..6513
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             6517..6543
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             6547..6573
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    6597..7185
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7251..7299
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7501..7929
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7943..8272
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8339..9133
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9309..9430
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9479..9495)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9503..9519
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9527..9557)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9572..9593
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9881..10469)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10640..11500)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11501..11605)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc tcctcgcgcc
      721 gcgggcggcg caccccttag cgcccgggcc gccgccgcca gccccccgcc gttccagacg
      781 ccgccgcggt gcccggtgcc gctgctgttg ctgctgctcc tgggggcggc gcgggccggc
      841 gccctggaga tccagcgtcg gttcccctcg cccacgccca ccaacaactt cgccctggac
      901 ggcgcggcgg ggaccgtgta cctggcggcc gtcaaccgcc tctatcagct gtcgggcgcc
      961 aacctgagcc tggaggccga ggcggccgtg ggcccggtgc ccgacagccc gctgtgtcac
     1021 gctccgcagc tgccgcaggc ctcgtgcgag cacccgcggc gcctcacgga caactacaac
     1081 aagatcctgc agctggaccc cggccagggc ctggtagtcg tgtgcgggtc catctaccag
     1141 ggcttctgcc agctgcggcg ccggggcaac atctcggccg tggccgtgcg cttcccgccc
     1201 gccgcgccgc ccgccgagcc cgtcacggtg ttccccagca tgctgaacgt ggcggccaac
     1261 cacccgaacg cgtccaccgt ggggctagtt ctgcctcccg ccgcgggcgc ggggggcagc
     1321 cgcctgctcg tgggcgccac gtacaccggt tacggcagct ccttcttccc gcgcaaccgc
     1381 agcctggagg accaccgctt cgagaacacg cccgagatcg ccatccgctc cctggacacg
     1441 cgcggcgacc tggccaagct cttcaccttc gacctcaacc cctccgacga caacatcctc
     1501 aagatcaagc agggcgccaa ggagcagcac aagctgggct tcgtgagcgc cttcctgcac
     1561 ccgtccgacc cgccgccggg tgcacagtcc tacgcgtacc tggcgctcaa cagcgaggcg
     1621 cgcgcgggcg acaaggagag ccaggcgcgg agcctgctgg cgcgcatctg cctgccccac
     1681 ggcgccggcg gcgacgccaa gaagctcacc gagtcctaca tccagttggg cttgcagtgc
     1741 gcgggcggcg cgggccgcgg cgacctctac agccgcctgg tgtcggtctt cccagcccgg
     1801 gagcggctct ttgctgtctt cgagcggccc caggggtccc ccgcggcccg cgctgctccg
     1861 gccgcactct gcgccttccg cttcgccgac gtgcgagccg ccatccgagc tgcgcgcacc
     1921 gcctgcttcg tggaaccggc gcccgacgtg gtggcggtgc tcgacagcgt ggtgcagggc
     1981 acgggaccgg cctgtgagcg caagctcaac atccagctcc agccagagca gctggactgt
     2041 ggagctgctc acctgcagca cccgctgtcc atcctgcagc ccctgaaggc cacgcccgtg
     2101 ttccgcgccc cgggcctcac ctccgtggcc gtggccagcg tcaacaacta cacagcggtc
     2161 ttcctgggca cggtcaacgg gaggcttctc aagatcaacc tgaacgagag catgcaggtg
     2221 gtgagcaggc gggtggtgac tgtggcctat ggggagcccg tgcaccatgt catgcagttt
     2281 gacccagcag actccggtta cctttacctg atgacgtccc accagatggc cagggtgaag
     2341 gtcgccgcct gcaacgtgca ctccacctgt ggggactgcg tgggtgcggc ggacgcctac
     2401 tgcggctggt gtgccctgga gacgcggtgc accttgcagc aggactgcac caattccagc
     2461 cagcagcatt tctggaccag tgccagcgag ggccccagcc gctgtcctgc catgaccgtc
     2521 ctgccttccg agatcgatgt gcgccaggag tacccaggca tgatcctgca gatctcgggc
     2581 agcctgccca gcctcagtgg catggagatg gcctgtgact atgggaacaa catccgcact
     2641 gtggctcggg tcccaggccc tgcctttggt caccagattg cctactgcaa cctcctgccg
     2701 agggaccagt ttccgccctt cccccccaac caggaccacg tgactgttga gatgtctgtg
     2761 agggtcaatg ggcggaacat cgtcaaggcc aatttcacca tctacgactg cagccgcact
     2821 gcacaagtgt acccccacac agcctgtacc agctgcctgt cggcacagtg gccctgtttc
     2881 tggtgcagcc agcagcactc ctgtgtttcc aaccagtctc ggtgcgaggc ctcaccaaac
     2941 cccacgagcc ctcaggactg cccccggacc ctgctctcac ccctggcacc cgtgcctacg
     3001 ggtggctccc agaacatcct ggtgcctctg gccaacactg cctttttcca gggtgcagcc
     3061 ctggagtgta gttttgggct ggaggagatc ttcgaggctg tgtgggtgaa tgagtctgtt
     3121 gtacgctgtg accaggtggt gctgcacacg acccggaaga gccaggtgtt cccgctcagc
     3181 ctccaactaa aggggcggcc agcccgattc ctggacagcc ctgagcccat gacagtcatg
     3241 gtctataact gtgccatggg cagccccgac tgttcccagt gcctgggccg cgaagacctg
     3301 ggtcacctgt gcatgtggag tgatggctgc cgcctgcggg ggcctctgca gcccatggct
     3361 ggcacctgcc ccgcccccga gatccacgcg attgagcccc tgagtggccc gttggacggt
     3421 gggaccctgc tgaccatccg aggaaggaac ctgggccggc ggctcagtga cgtggcccac
     3481 ggcgtgtgga ttggtggtgt ggcctgtgag ccactgcctg acagatacac ggtgtcggag
     3541 gagatcgtgt gtgtcacagg gccagcccca ggaccactct caggtgtggt gaccgtgaac
     3601 gcctctaagg agggcaagtc ccgggaccgc ttctcctacg tgctgcccct ggtccactcc
     3661 ctggagccta ccatgggccc caaggccggg ggcaccagga tcaccatcca tgggaatgac
     3721 ctccatgtag gctccgagct ccaggtcctg gtgaacgaca cagacccctg cacggagctg
     3781 atgcgcacag ataccagcat cgcctgcacc atgcctgagg gggccctgcc ggctccggtg
     3841 cctgtgtgtg tgcgcttcga gcgtcggggc tgcgtgcacg gcaacctcac cttctggtac
     3901 atgcagaacc cggtcatcac ggccatcagt ccccgccgca gccctgtcag tggcggcagg
     3961 accatcacag tggctggtga gcgtttccac atggtgcaga atgtgtccat ggccgtccac
     4021 cacattggcc gggagcccac gctctgcaag gttctcaact ccaccctcat cacctgcccg
     4081 tcccccgggg ccctgagcaa cgcatcagcg ccagtggact tcttcatcaa tgggcgggcc
     4141 tacgcagacg aggtggctgt ggctgaggag ctactggacc ccgaggaggc acagcggggc
     4201 agcaggttcc gcctggacta cctccccaac ccacagttct ctacggccaa gagggagaag
     4261 tggatcaagc accaccccgg ggagcctctc accctcgtta tccacaagga gcaggacagc
     4321 ctggggctcc agagtcacga gtaccgggtc aagataggcc aagtaagctg cgacatccag
     4381 attgtctctg acagaatcat ccactgctcg gtcaacgagt ccctgggcgc ggccgtgggg
     4441 cagctgccca tcacaatcca ggtagggaac ttcaaccaga ccatcgccac actgcagctg
     4501 gggggcagcg agacggccat catcgtgtcc atcgtcatct gcagcgtcct gctgctgctc
     4561 tccgtggtgg ccctgttcgt cttctgtacc aagagccgac gtgctgagcg ttactggcag
     4621 aagacgctgc tgcagatgga ggagatggaa tctcagatcc gagaggaaat ccgcaaaggc
     4681 ttcgctgagc tgcagacaga catgacagat ctcaccaagg agctgaaccg cagccagggc
     4741 atccccttcc tggagtataa gcacttcgtg acccgcacct tcttccccaa gtgttcctcc
     4801 ctttatgaag agcgttacgt gctgccctcc cagaccctca actcccaggg cagctcccag
     4861 gcacaggaaa cccacccact gctgggagag tggaagattc ctgagagctg ccggcccaac
     4921 atggaagagg gaattagctt gttctcctca ctactcaaca acaagcactt cctcatcgtc
     4981 tttgtccacg cgctggagca gcagaaggac tttgcggtgc gcgacaggtg cagcctggcc
     5041 tcgctgctga ccatcgcgct gcacggcaag ctggagtact acaccagcat catgaaggag
     5101 ctgctggtgg acctcattga cgcctcggcc gccaagaacc ccaagctcat gctgcggcgc
     5161 acagagtctg tggtggagaa gatgctcacc aactggatgt ccatctgcat gtacagctgt
     5221 ctgcgggaga cggtggggga gccattcttc ctgctgctgt gtgccatcaa gcagcaaatc
     5281 aacaagggct ccatcgacgc catcacaggc aaggcccgct acacactcag tgaggagtgg
     5341 ctgctgcggg agaacatcga ggccaagccc cggaacctga acgtgtcctt ccagggctgt
     5401 ggcatggact cgctgagcgt gcgggccatg gacaccgaca cgctgacaca ggtcaaggag
     5461 aagatcctgg aggccttctg caagaatgtg ccctactccc agtggccgcg tgcagaggac
     5521 gtcgaccttg agtggttcgc ctccagcaca cagagctaca tccttcggga cctggacgac
     5581 acctcagtgg tggaagacgg ccgcaagaag cttaacacgc tggcccatta caagatccct
     5641 gaaggtgcct ccctggccat gagtctcata gacaagaagg acaacacact gggccgagtg
     5701 aaagacttgg acacagagaa gtatttccat ttggtgctgc ctacggacga gctggcggag
     5761 cccaagaagt ctcaccggca gagccatcgc aagaaggtgc tcccggaaat ctacctgacc
     5821 cgcctgctct ccaccaaggg cacgttgcag aagtttctgg atgacctgtt caaggccatt
     5881 ctgagtatcc gtgaagacaa gcccccactg gctgtcaagt actttttcga cttcctggag
     5941 gagcaggctg agaagagggg aatctccgac cccgacaccc tacacatctg gaagaccaac
     6001 agccttcctc tccggttctg ggtgaacatc ctgaagaacc cccagtttgt ctttgacatc
     6061 gacaagacag accacatcga cgcctgcctt tcagtcatcg cgcaggcctt catcgacgcc
     6121 tgctccatct ctgacctgca gctgggcaag gattcgccaa ccaacaagct cctctacgcc
     6181 aaggagattc ctgagtaccg gaagatcgtg cagcgctact acaagcagat ccaggacatg
     6241 acgccgctca gcgagcaaga gatgaatgcc catctggccg aggagtcgag gaaataccag
     6301 aatgagttca acaccaatgt ggccatggca gagatttata agtacgccaa gaggtatcgg
     6361 ccgcagatca tggccgcgct ggaggccaac cccacggccc ggaggacaca actgcagcac
     6421 aagtttgagc aggtggtggc tttgatggag gacaacatct acgagtgcta cagtgaggcc
     6481 ctcgagtacc catacgacgt acctgattat gcaacttatc cttatgatgt acctgattat
     6541 gctagctatc cttatgatgt cccagactac gcatagtgag ggcccgatat ctcgacaatc
     6601 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6661 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6721 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6781 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6841 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6901 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6961 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     7021 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7081 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7141 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga aacgggggag
     7201 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     7261 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     7321 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     7381 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     7441 tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc
     7501 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7561 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7621 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta
     7681 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7741 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7801 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7861 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7921 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7981 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     8041 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8101 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8161 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     8221 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     8281 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     8341 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     8401 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     8461 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     8521 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     8581 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     8641 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     8701 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     8761 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     8821 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     8881 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     8941 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     9001 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     9061 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     9121 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc
     9181 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     9241 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     9301 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9361 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9421 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     9481 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     9541 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9601 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     9661 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     9721 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9781 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9841 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9901 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9961 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10021 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
    10081 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10141 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10201 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10261 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    10321 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    10381 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    10441 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    10501 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    10561 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    10621 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    10681 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    10741 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    10801 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    10861 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10921 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10981 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    11041 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    11101 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    11161 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    11221 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    11281 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    11341 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    11401 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    11461 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    11521 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    11581 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
    11641 gatcgggaga tctcccgatc ccctatggtc gactctcagt acaatctgct ctgatgccgc
    11701 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
    11761 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
    11821 g
//
