LOCUS       V025505                 8320 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025505
VERSION     V025505
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2979
                     /label="PLOD2(NM_182943)"
                     /note="PLOD2(NM_182943)"
                     /gene="PLOD2"
     CDS             2986..3012
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3016..3042
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3046..3072
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3096..3684
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3750..3798
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4000..4428
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4442..4771
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4838..5632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5808..5929
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5978..5994)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6002..6018
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6026..6056)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6071..6092
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6380..6968)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7139..7999)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8000..8104)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggg gggatgcacg
      721 gtgaagcctc agctgctgct cctggcgctc gtcctccacc cctggaatcc ctgtctgggt
      781 gcggactcgg agaagccctc gagcatcccc acagataaat tattagtcat aactgtagca
      841 acaaaagaaa gtgatggatt ccatcgattt atgcagtcag ccaaatattt caattatact
      901 gtgaaggtcc ttggtcaagg agaagaatgg agaggtggtg atggaattaa tagtattgga
      961 gggggccaga aagtgagatt aatgaaagaa gtcatggaac actatgctga tcaagatgat
     1021 ctggttgtca tgtttactga atgctttgat gtcatatttg ctggtggtcc agaagaagtt
     1081 ctaaaaaaat tccaaaaggc aaaccacaaa gtggtctttg cagcagatgg aattttgtgg
     1141 ccagataaaa gactagcaga caagtatcct gttgtgcaca ttgggaaacg ctatctgaat
     1201 tcaggaggat ttattggcta tgctccatat gtcaaccgta tagttcaaca atggaatctc
     1261 caggataatg atgatgatca gctcttttac actaaagttt acattgatcc actgaaaagg
     1321 gaagctatta acatcacatt ggatcacaaa tgcaaaattt tccagacctt aaatggagct
     1381 gtagatgaag ttgttttaaa atttgaaaat ggcaaagcca gagctaagaa tacattttat
     1441 gaaacattac cagtggcaat taatggaaat ggacccacca agattctcct gaattatttt
     1501 ggaaactatg tacccaattc atggacacag gataatggct gcactctttg tgaattcgat
     1561 acagtcgact tgtctgcagt agatgtccat ccaaacgtat caataggtgt ttttattgag
     1621 caaccaaccc cttttctacc tcggtttctg gacatattgt tgacactgga ttacccaaaa
     1681 gaagcactta aactttttat tcataacaaa gaagtttatc atgaaaagga catcaaggta
     1741 ttttttgata aagctaagca tgaaatcaaa actataaaaa tagtaggacc agaagaaaat
     1801 ctaagtcaag cggaagccag aaacatggga atggactttt gccgtcagga tgaaaagtgt
     1861 gattattact ttagtgtgga tgcagatgtt gttttgacaa atccaaggac tttaaaaatt
     1921 ttgattgaac aaaacagaaa gatcattgct cctcttgtaa ctcgtcatgg aaagctgtgg
     1981 tccaatttct ggggagcatt gagtcctgat ggatactatg cacgatctga agattatgtg
     2041 gatattgttc aagggaatag agtaggagta tggaatgtcc catatatggc taatgtgtac
     2101 ttaattaaag gaaagacact ccgatcagag atgaatgaaa ggaactattt tgttcgtgat
     2161 aaactggatc ctgatatggc tctttgccga aatgctagag aaatgacttt acaaagggaa
     2221 aaagactccc ctactccgga aacattccaa atgctcagcc ccccaaaggg tgtatttatg
     2281 tacatttcta atagacatga atttggaagg ctattatcca ctgctaatta caatacttcc
     2341 cattataaca atgacctctg gcagattttt gaaaatcctg tggactggaa ggaaaagtat
     2401 ataaaccgtg attattcaaa gattttcact gaaaatatag ttgaacagcc ctgtccagat
     2461 gtcttttggt tccccatatt ttctgaaaaa gcctgtgatg aattggtaga agaaatggaa
     2521 cattacggca aatggtctgg gggaaaacat catgatagcc gtatatctgg tggttatgaa
     2581 aatgtcccaa ctgatgatat ccacatgaag caagttgatc tggagaatgt atggcttcat
     2641 tttatccggg agttcattgc accagttaca ctgaaggtct ttgcaggcta ttatacgaag
     2701 ggatttgcac tactgaattt tgtagtaaaa tactcccctg aacgacagcg ttctcttcgt
     2761 cctcatcatg atgcttctac atttaccata aacattgcac ttaataacgt gggagaagac
     2821 tttcagggag gtggttgcaa atttctaagg tacaattgct ctattgagtc accacgaaaa
     2881 ggctggagct tcatgcatcc tgggagactc acacatttgc atgaaggact tcctgttaaa
     2941 aatggaacaa gatacattgc agtgtcattt atagatcccc tcgagtaccc atacgacgta
     3001 cctgattatg caacttatcc ttatgatgta cctgattatg ctagctatcc ttatgatgtc
     3061 ccagactacg catagtgagg gcccgatatc tcgacaatca acctctggat tacaaaattt
     3121 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3181 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3241 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3301 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3361 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3421 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3481 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     3541 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3601 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3661 tctccctttg ggccgcctcc ccgcctggaa acgggggagg ctaactgaaa cacggaagga
     3721 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     3781 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     3841 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     3901 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     3961 tagcagatct gcgcagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     4021 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4081 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4141 aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4201 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4261 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4321 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg
     4381 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     4441 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4501 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4561 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4621 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4681 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     4741 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     4801 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     4861 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4921 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     4981 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     5041 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5101 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5161 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5221 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5281 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     5341 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     5401 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5461 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5521 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     5581 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     5641 ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     5701 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     5761 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     5821 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5881 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     5941 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6001 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6061 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6121 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6181 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6241 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6301 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6361 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6421 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6481 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6541 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     6601 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6661 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6721 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6781 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     6841 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6901 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6961 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7021 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7081 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7141 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7201 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7261 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7321 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7381 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7441 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7501 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7561 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7621 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7681 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7741 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7801 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7861 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7921 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7981 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8041 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8101 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     8161 cctatggtcg actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     8221 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     8281 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
//
