LOCUS       V038951                 9313 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038951
VERSION     V038951
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3972
                     /label="PDGFRA(NM_006206)"
                     /note="PDGFRA(NM_006206)"
                     /gene="PDGFRA"
     CDS             3979..4005
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4009..4035
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4039..4065
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    4089..4677
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4743..4791
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4993..5421
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5435..5764
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5831..6625
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6801..6922
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6971..6987)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6995..7011
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7019..7049)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7064..7085
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7373..7961)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8132..8992)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8993..9097)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggg gacttcccat
      721 ccggcgttcc tggtcttagg ctgtcttctc acagggctga gcctaatcct ctgccagctt
      781 tcattaccct ctatccttcc aaatgaaaat gaaaaggttg tgcagctgaa ttcatccttt
      841 tctctgagat gctttgggga gagtgaagtg agctggcagt accccatgtc tgaagaagag
      901 agctccgatg tggaaatcag aaatgaagaa aacaacagcg gcctttttgt gacggtcttg
      961 gaagtgagca gtgcctcggc ggcccacaca gggttgtaca cttgctatta caaccacact
     1021 cagacagaag agaatgagct tgaaggcagg cacatttaca tctatgtgcc agacccagat
     1081 gtagcctttg tacctctagg aatgacggat tatttagtca tcgtggagga tgatgattct
     1141 gccattatac cttgtcgcac aactgatccc gagactcctg taaccttaca caacagtgag
     1201 ggggtggtac ctgcctccta cgacagcaga cagggcttta atgggacctt cactgtaggg
     1261 ccctatatct gtgaggccac cgtcaaagga aagaagttcc agaccatccc atttaatgtt
     1321 tatgctttaa aagcaacatc agagctggat ctagaaatgg aagctcttaa aaccgtgtat
     1381 aagtcagggg aaacgattgt ggtcacctgt gctgttttta acaatgaggt ggttgacctt
     1441 caatggactt accctggaga agtgaaaggc aaaggcatca caatgctgga agaaatcaaa
     1501 gtcccatcca tcaaattggt gtacactttg acggtccccg aggccacggt gaaagacagt
     1561 ggagattacg aatgtgctgc ccgccaggct accagggagg tcaaagaaat gaagaaagtc
     1621 actatttctg tccatgagaa aggtttcatt gaaatcaaac ccaccttcag ccagttggaa
     1681 gctgtcaacc tgcatgaagt caaacatttt gttgtagagg tgcgggccta cccacctccc
     1741 aggatatcct ggctgaaaaa caatctgact ctgattgaaa atctcactga gatcaccact
     1801 gatgtggaaa agattcagga aataaggtat cgaagcaaat taaagctgat ccgtgctaag
     1861 gaagaagaca gtggccatta tactattgta gctcaaaatg aagatgctgt gaagagctat
     1921 acttttgaac tgttaactca agttccttca tccattctgg acttggtcga tgatcaccat
     1981 ggctcaactg ggggacagac ggtgaggtgc acagctgaag gcacgccgct tcctgatatt
     2041 gagtggatga tatgcaaaga tattaagaaa tgtaataatg aaacttcctg gactattttg
     2101 gccaacaatg tctcaaacat catcacggag atccactccc gagacaggag taccgtggag
     2161 ggccgtgtga ctttcgccaa agtggaggag accatcgccg tgcgatgcct ggctaagaat
     2221 ctccttggag ctgagaaccg agagctgaag ctggtggctc ccaccctgcg ttctgaactc
     2281 acggtggctg ctgcagtcct ggtgctgttg gtgattgtga tcatctcact tattgtcctg
     2341 gttgtcattt ggaaacagaa accgaggtat gaaattcgct ggagggtcat tgaatcaatc
     2401 agcccagatg gacatgaata tatttatgtg gacccgatgc agctgcctta tgactcaaga
     2461 tgggagtttc caagagatgg actagtgctt ggtcgggtct tggggtctgg agcgtttggg
     2521 aaggtggttg aaggaacagc ctatggatta agccggtccc aacctgtcat gaaagttgca
     2581 gtgaagatgc taaaacccac ggccagatcc agtgaaaaac aagctctcat gtctgaactg
     2641 aagataatga ctcacctggg gccacatttg aacattgtaa acttgctggg agcctgcacc
     2701 aagtcaggcc ccatttacat catcacagag tattgcttct atggagattt ggtcaactat
     2761 ttgcataaga atagggatag cttcctgagc caccacccag agaagccaaa gaaagagctg
     2821 gatatctttg gattgaaccc tgctgatgaa agcacacgga gctatgttat tttatctttt
     2881 gaaaacaatg gtgactacat ggacatgaag caggctgata ctacacagta tgtccccatg
     2941 ctagaaagga aagaggtttc taaatattcc gacatccaga gatcactcta tgatcgtcca
     3001 gcctcatata agaagaaatc tatgttagac tcagaagtca aaaacctcct ttcagatgat
     3061 aactcagaag gccttacttt attggatttg ttgagcttca cctatcaagt tgcccgagga
     3121 atggagtttt tggcttcaaa aaattgtgtc caccgtgatc tggctgctcg caacgtcctc
     3181 ctggcacaag gaaaaattgt gaagatctgt gactttggcc tggccagaga catcatgcat
     3241 gattcgaact atgtgtcgaa aggcagtacc tttctgcccg tgaagtggat ggctcctgag
     3301 agcatctttg acaacctcta caccacactg agtgatgtct ggtcttatgg cattctgctc
     3361 tgggagatct tttcccttgg tggcacccct taccccggca tgatggtgga ttctactttc
     3421 tacaataaga tcaagagtgg gtaccggatg gccaagcctg accacgctac cagtgaagtc
     3481 tacgagatca tggtgaaatg ctggaacagt gagccggaga agagaccctc cttttaccac
     3541 ctgagtgaga ttgtggagaa tctgctgcct ggacaatata aaaagagtta tgaaaaaatt
     3601 cacctggact tcctgaagag tgaccatcct gctgtggcac gcatgcgtgt ggactcagac
     3661 aatgcataca ttggtgtcac ctacaaaaac gaggaagaca agctgaagga ctgggagggt
     3721 ggtctggatg agcagagact gagcgctgac agtggctaca tcattcctct gcctgacatt
     3781 gaccctgtcc ctgaggagga ggacctgggc aagaggaaca gacacagctc gcagacctct
     3841 gaagagagtg ccattgagac gggttccagc agttccacct tcatcaagag agaggacgag
     3901 accattgaag acatcgacat gatggatgac atcggcatag actcttcaga cctggtggaa
     3961 gacagcttcc tgctcgagta cccatacgac gtacctgatt atgcaactta tccttatgat
     4021 gtacctgatt atgctagcta tccttatgat gtcccagact acgcatagtg agggcccgat
     4081 atctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4141 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4201 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4261 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4321 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4381 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4441 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     4501 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4561 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4621 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     4681 gaaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     4741 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     4801 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     4861 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     4921 tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc tggggctcta
     4981 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5041 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     5101 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag
     5161 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     5221 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5281 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     5341 cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt
     5401 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     5461 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     5521 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5581 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5641 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5701 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5761 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     5821 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5881 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5941 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     6001 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     6061 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     6121 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     6181 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6241 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     6301 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     6361 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     6421 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     6481 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6541 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6601 tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag
     6661 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6721 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6781 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     6841 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6901 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     6961 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7021 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7081 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7141 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7201 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7261 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7321 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7381 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7441 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7501 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7561 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7621 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7681 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7741 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7801 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7861 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7921 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7981 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8041 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8101 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8161 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8221 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8281 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8341 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8401 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8461 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8521 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8581 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8641 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8701 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8761 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8821 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8881 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8941 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9001 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9061 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9121 ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca gtacaatctg
     9181 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     9241 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     9301 gaatctgctt agg
//
