LOCUS       V029639                 6000 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029639
VERSION     V029639
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2147
                     /label="P2ry1(NM_008772)"
                     /note="P2ry1(NM_008772)"
                     /gene="P2ry1"
     CDS             2166..2195
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2214..2237
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2257..2273)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2284..2906
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3055..3643)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3972..4019
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4251..5045)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5080..5437)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5439..5543)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5570..5998
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaccgaggtg ccttggtcgg ttgtccccaa cgggacggat gctgcctttc
     1081 tggcgggcct gggctcgctt tggggaaaca gtactgtcgc ctcaactgca gcagtttcct
     1141 cttcattcca atgtgccctg accaagaccg gtttccagtt ctactacctg ccggctgtct
     1201 acattttagt gttcatcata ggcttcctag gcaacagcgt ggctatctgg atgttcgttt
     1261 tccacatgaa gccttggagc ggcatctccg tgtacatgtt caatttggct ctggctgact
     1321 ttttatacgt gctcacccta ccagccctca tcttctacta cttcaacaag actgactgga
     1381 tcttcgggga tgctatgtgc aagctgcaga gattcatctt ccacgtaaat ctctacggta
     1441 gcatcttgtt cctcacctgc atcagcgcac acaggtacag tggcgtggtg taccctctca
     1501 agtctctggg caggctcaag aagaagaatg ccatttatgt cagcgtgctg gtgtggctca
     1561 ttgtggtggt ggccatctcc cctattctct tctactctgg cactgggact cggaaaaaca
     1621 aaactgtcac ctgctatgac accacgtcca atgattacct gcgaagttat ttcatctaca
     1681 gtatgtgcac gactgtggcc atgttctgca tccctttggt gctgatcttg ggctgttatg
     1741 gattaattgt taaagctttg atttacaatg acctggacaa ctctccgctc cggaggaaat
     1801 ccatttacct ggtgataatt gtcctgacgg tgtttgctgt gtcttatatc cctttccatg
     1861 tgatgaaaac gatgaatttg cgagcacggt tggatttcca gaccccagaa atgtgtgatt
     1921 tcaacgacag ggtttatgcc acttatcagg taacaagagg tctagcaagt ctcaacagct
     1981 gtgtggaccc cattctttat ttcttggctg gagatacatt cagaaggaga ctgtcccgag
     2041 ccaccaggaa agcttccagg aggagtgagg ccaatttaca atccaagagt gaagaaatga
     2101 ctctcaatat tttatctgag ttcaagcaga atggagacac gagtttgacg cgtacgcggc
     2161 cgctcgagca gaaactcatc tcagaagagg atctggcagc aaatgatatc ctggattaca
     2221 aggatgacga cgataaggtt taaacggccg gccgcggtca tagctgtttc ctgaacagat
     2281 cccgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     2341 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     2401 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     2461 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     2521 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     2581 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     2641 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     2701 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     2761 ctgattttaa aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc
     2821 catgcccaac cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg
     2881 tccactcagt agatgcctgt tgaattgggt acgcggccag cggcgagcgg tatcagctca
     2941 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3001 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3061 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3121 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3181 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3241 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3301 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3361 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3421 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3481 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3541 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3601 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3661 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3721 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3781 ctaaagtata tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc
     3841 gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc
     3901 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac
     3961 cccgcgttta tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt
     4021 catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg
     4081 gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg
     4141 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
     4201 tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac
     4261 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     4321 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     4381 gctatgtcct gatagcgatc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     4441 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
     4501 tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     4561 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     4621 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     4681 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     4741 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     4801 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     4861 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     4921 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     4981 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     5041 atcatgcgaa acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc
     5101 aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc
     5161 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
     5221 gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct
     5281 ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt
     5341 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa
     5401 ctgacacaca ttccacagct ggttctttcc gcctcaggac tcttcctttt tcaatattat
     5461 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5521 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     5581 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5641 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5701 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5761 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5821 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5881 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5941 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
//
