LOCUS       V030908                 5862 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030908
VERSION     V030908
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2009
                     /label="Or6a2(NM_010983)"
                     /note="Or6a2(NM_010983)"
                     /gene="Or6a2"
     CDS             2028..2057
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2076..2099
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2119..2135)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2146..2768
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2917..3505)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3834..3881
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4113..4907)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4942..5299)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5301..5405)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5432..5860
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagcgaagg aaccacactg ggagagtgag tgaatttgtg ttgctgggtt
     1081 tcccagctcc tgccccactg cgggcactac tatttttcct ttctctgttg gcctacgtgt
     1141 tggtgctgac tgaaaacata ctcatcatta cagcaattag gaaccacccc accctccaca
     1201 aacccatgta ttttttcttg gctaatatgt cattcctgga gatttggtat gtcactgtta
     1261 cgattcctaa gatgcttgct ggcttcattg gttccgagga gaatcatgga cagctgatct
     1321 cctttgaggc atgcatgaca cagctctact ttttcctagg cttgggttgc acagagtgtg
     1381 tccttcttgc tgtcatggcc tatgaccgct atgtggccat ctgtcaccca ctccactatc
     1441 ctgtcattgt cagtagccgg ctatgtgtgc agatggcagc tggatcctgg gctggaggtt
     1501 ttggtatctc catggttaaa gttttcctca tttctcgcct gtcttactgt ggccccaaca
     1561 ccatcaacca ctttttctgt gatgtttctc cattgctcaa cttgtcatgc actgacatgt
     1621 ccacagcaga gcttacagac tttatcctgg ccatttttat tctgctgggg ccactctctg
     1681 tcactggggc ttcctatatg gccatcacag gtgcagtgat gcgcatcccc tcagctgctg
     1741 gccgccataa ggccttttca acctgtgcct cccacctcac tgttgtgatt atcttctatg
     1801 cagccagtat tttcatctat gccaggccta aggcactctc agcttttgac accaacaagc
     1861 tggtctctgt actctacgct gtcattgtac cattgctcaa tcccatcatc tactgcttgc
     1921 gcaatcaaga agtcaaaaaa gccctacgtc gcactctgca cctggcccaa ggccaggacg
     1981 ccaataccaa gaaatccagc agagatggta cgcgtacgcg gccgctcgag cagaaactca
     2041 tctcagaaga ggatctggca gcaaatgata tcctggatta caaggatgac gacgataagg
     2101 tttaaacggc cggccgcggt catagctgtt tcctgaacag atcccgggtg gcatccctgt
     2161 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     2221 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     2281 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     2341 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     2401 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     2461 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     2521 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     2581 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta
     2641 taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga
     2701 catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct
     2761 gttgaattgg gtacgcggcc agcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2821 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2881 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2941 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3001 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3061 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3121 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3181 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3241 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3301 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3361 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3421 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3481 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3541 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3601 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3661 acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc
     3721 cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg
     3781 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
     3841 cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
     3901 cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag
     3961 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     4021 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     4081 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     4141 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     4201 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcga
     4261 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     4321 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc
     4381 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     4441 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     4501 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     4561 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     4621 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     4681 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     4741 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     4801 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     4861 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     4921 catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     4981 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     5041 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     5101 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     5161 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     5221 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     5281 ctggttcttt ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt
     5341 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5401 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     5461 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5521 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5581 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5641 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5701 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5761 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5821 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt tt
//
