LOCUS       V031101                 5787 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031101
VERSION     V031101
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1934
                     /label="Or4c3d(NM_020515)"
                     /note="Or4c3d(NM_020515)"
                     /gene="Or4c3d"
     CDS             1953..1982
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2001..2024
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2044..2060)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2071..2693
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2842..3430)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3759..3806
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4038..4832)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4867..5224)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5226..5330)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5357..5785
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggatagtcca cgcaatgtca cagaattttt catgctgggt ctctcccaga
     1081 acccccaggt gcagaggatg ttgtttgggc tttttctgct ggtcttcctg gtctctgttg
     1141 ggggcaacat gctcatcatt atcacgatta ccttcagccc caccctaggt tctcctatgt
     1201 actttttcct ctcatatctt tcctttattg atacttgcta ttcctcttgt atgacaccca
     1261 aactcatcgc tgactccctg catgagggaa gagccatctc ttttgagggc tgcttggccc
     1321 agttctttgt ggctcattta ttgggaggaa ctgagatcat tctgctcaca gtgatggcct
     1381 atgaccgcta tgtggccatc tgcaagcccc tacactatac caccaccatg accaggcatg
     1441 tctgtattgt gctggtggca gtggcttggc tgggtggcat cctgcattca acagctcagc
     1501 tctttctggt ccttcagttg cccttctgtg gtcccaatgt gataaaccac tttgtgtgtg
     1561 acttgtatcc tttactggag ctggcctgca ctgacacata tgtcattggc ctgctggtgg
     1621 tggccaacag tggtgtgatt tgcctgttga acttcctcat gctggctgcc tcgtacattg
     1681 tcatcctgcg cactttgagg tcccacagtg cagagggaag acggaaagct ctgtctacct
     1741 gtggagccca cttcactgtt gttgctttgt tctttgtgcc ctgtatattt atttacatga
     1801 gaccatcatc tactctgtcc atagacaaaa tagtagctgt gttttattgt attttgacac
     1861 ccatgttcaa ccccctgatt tatactctga gaaatgcaga ggtgaaaaat gccatgaaga
     1921 atctctggag aaaaacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc
     1981 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc
     2041 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg
     2101 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     2161 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     2221 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     2281 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     2341 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc
     2401 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc
     2461 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg
     2521 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac
     2581 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg
     2641 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg
     2701 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2761 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2821 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2881 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2941 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3001 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3061 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3121 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3181 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3241 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3301 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3361 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3421 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3481 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3541 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc
     3601 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg
     3661 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc
     3721 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg
     3781 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt
     3841 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg
     3901 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     3961 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     4021 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     4081 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     4141 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc
     4201 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     4261 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg
     4321 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     4381 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     4441 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     4501 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     4561 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     4621 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg
     4681 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     4741 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     4801 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     4861 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     4921 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     4981 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     5041 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     5101 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     5161 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc
     5221 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5281 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5341 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5401 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5461 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5521 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5581 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5641 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5701 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5761 atttaacaaa aatttaacgc gaatttt
//
