LOCUS       V046115                12399 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046115
VERSION     V046115
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..7079
                     /label="Numa1(NM_001403544)"
                     /note="Numa1(NM_001403544)"
                     /gene="Numa1"
     CDS             7086..7151
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    7175..7763
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7829..7877
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8079..8507
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8521..8850
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8917..9711
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9887..10008
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10057..10073)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10081..10097
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10105..10135)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10150..10171
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10459..11047)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11218..12078)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12079..12183)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgac actccatgcc acccgggcag
      781 ctacgctcct ttcttgggtg aacagtctgc atgttgctga ccccgtggag acagtgctgc
      841 agctccagga ctgcagcatc ttcatcaaga ttattaacac catccatgac actaaagagg
      901 ggcaacaaat cctacagcag ccactaccgg aaagactaga ctttgtttgc agttttctac
      961 agaaaaatcg gaaacatcct tcatctacac aatgcctggt atctgtgcag aaagtgatag
     1021 agggatccga gatggaactg gccaagatga taatgctgtt tttatatcag tccaccatga
     1081 gctccagaaa cctccgggac tgggagcagt ttgaatatgg ggttcaggct gagttagcgg
     1141 tcatcctgaa gtttatgctg gatcatgagg agtcgctaaa ccttactgag gacttagaga
     1201 gtttcttgga gaaagttcct tacacccatg ccagcaccct ctcagaagag ctttctccac
     1261 ccagccacca gaccaagagg aagattcgct tcctagaaat acagaggatt gcttcatctt
     1321 ccagtgagaa caacttcctc tcaggctctc catcctcccc tatgggcgac atactgcaga
     1381 ccccacagtt ccagatgaga cgactgaaga agcagctggc agacgagagg agcaacagag
     1441 atgacctgga gctggagctg tctgagagcc tcaagctcct cactgagaag gacgcgcaga
     1501 tcgccatgat gcagcagcgc attgatcacc tggctctgct gaatgagaag caggcagcca
     1561 gctcccagga gcccagtgag cttgaggagc tccgaggcaa gaatgaaagc ctcactgtgc
     1621 ggctgcatga gactttgaag cagtgtcaga acctgaagac cgagaagagc cagatggatc
     1681 gaaagattag ccagctttct gaggagaatg gggacctttc ctttaaggtt cgagagtttg
     1741 caaaccactt gcaacaactt cagggtgcat tcaacgacct gatagaggag cacagcaagg
     1801 cttctcagga gtgggcagag aagcaggctc gtctggaaaa tgagctcagc acagccctcc
     1861 aggataagaa atgtcttgaa gagaagaatg aaattcttca gggaaagctt tcccagctgg
     1921 aagatcaagc aactcggctg caggagagcc cagccccgga gaagggtgag gtgctaggag
     1981 acgccttgca gctggacact ctaaagcaag aggcagccaa acttgccaca gacaacaccc
     2041 agctccaaac ccgcgtagag acactggagt gtgaacgtgg caagcaggaa gcccagctgc
     2101 ttgctgaacg gagccgcttt gaagacgaaa agcagcagct ggccagcttg attgctgacc
     2161 ttcagagctc cgtctctaac ctcagccagg ccaaagagga gttagagcaa gcctcccagg
     2221 cccaaggggc tcagctcact gcccagttga cctctatgac agggctcaat gccaccctgc
     2281 agcagcgaga tcaagagctg gccagtctga aagaacaggc caaaaaggaa caggctcaga
     2341 tgctacagac catgcaagag caagaacagg ctgcccaggg cctccgccag caggtggagc
     2401 agctgagcag cagcctgaag ctgaaggagc agcaattaga agaggcagcc aaggagcagg
     2461 aggcaaccag acaggatcac gcccagcaac tggccatcgt tgctgaggct cgagaggcct
     2521 ctctaaggga acgggatact gctcgccagc agctagaaac agtggagaag gagaaggatg
     2581 ccaagctaga gagtctgcag cagcaacttc aggctgccaa tgatgctagg gacaatgcgc
     2641 agacctcggt cacacaggct cagcaggaaa aggcagagct gagccaaaag ataggagagc
     2701 ttcatgcctg cattgaagct tcccaccagg aacagcgtca ggttcaagct cgtgtgacag
     2761 agctagaagc ccagctgaaa gctgagcagc aaaaaacaac tgagcgagaa aaggtggttc
     2821 aggagaaggc ccagctccag gagcagctcc gggcccttga ggagtctttg aagattacca
     2881 aaggcagcct tgaagaagag aaacgcaggg ctgcagatgc cctgaaggag cagcagtgcc
     2941 gtgccactga gatggaggca gagtctcgaa gcctgatgga gcagcgggag cgggaacaga
     3001 aggagttaga gcaagagaag gctgggcgaa aggggctgga ggcccggata cagcagcttg
     3061 aagaggctca tcaggctgag actgaagcct tgcggcatga gctggcagag gccacagctt
     3121 cccagcacag agctgagagt gagtgtgagc ggctcatcag agaggttgag agccggcaga
     3181 agcggtttga agctagacag caagaggagg caaggtacgg tgccatgttc caggaacagc
     3241 tgatggcttt gaaaggggag aagactggcc aagaggtcca ggaggaggca gtagagatcc
     3301 acagtgaagg ccagccaggc cagcagcaga gtcagctagc ccagcttcat gccagtctgg
     3361 ccaaagccat ccagcaggtc caggagaagg aagtgagagc acagaagctg gtagatgatc
     3421 tctcagctct gcaggagaag atggctgcca ctaacaagga ggtggcctgt ctgaagacct
     3481 tggtgctcaa ggcaggtgag cagcaggaaa cagcctcact tgagctgctc aaagagcccc
     3541 cgagagctgc aaacagagcg tctgaccagc taggagaaca gcagggacgg ccattctcta
     3601 gtacacacgc agcagtaaaa gctatggagc gtgaagctga gcagatgggc ggtgaactgg
     3661 agaggctgcg ggcagcactg atcaagagcc agggccagca gcaagaggag cgtgggcaac
     3721 aggagaggga ggtagcacgg ctgacccagg agcggggcca ggcccaggct gacctggctc
     3781 aggagaaggc agccaaggca gagctggaga tgcgactgca gaacaccctc aatgagcagc
     3841 gtgtggagtt tgctgcccta caggaagcac tggcccatgc cctgacagaa aaggaaggga
     3901 cagaccaaga gcttgccaaa cttcgagggc aggaggcagc tcaaagaaca gagctgaagg
     3961 agcttcaaca gactttggaa cagctgaaaa tacagttagt gaagaaagaa aaagagcacc
     4021 ctgcaggggg tgccagtgga gaagatgctt ctggcccagg aacccagtcg gagacagctg
     4081 gaaagacaga tgcaccaggc cctgagttgc aggcacttcg ggcagagatc agtaagctag
     4141 agcagcagtg tcagcagcag cagcaacagg tggaaggcct gacacacagc ctcaagtctg
     4201 agcgggcttg ccgggccgag caagacaaag ccctggagac actgcagggc cagttagaag
     4261 agaaggctcg agagctaggg cataatcagg ctgcctcagc ttcagctcag agagagttgc
     4321 aagccctccg tgctaaagcc caggaccata gcaaagctga ggaagagtgg aaggcccagg
     4381 tagcccgggg ccagcaggag gctgaaagaa aaagcagtct tatcagcagc ttggaagagg
     4441 aggtatccat cctgaatcgc caagtcctag agaaggaagg ggagagcaag gagttgaagc
     4501 gcctggttgt agctgaatcc gagaagagcc agaagctgga agagaggctg cgcctgcttc
     4561 aagtagaaac agccagcaat agtgccaggg cagcagaacg cagctcagct ctacgagagg
     4621 aggtccagag cctccgggaa gaggtggaga aacagcgtgt agtttcagag aactcgcggc
     4681 aggagttggc ctcacaagca gagcgagctg aggagttggg ccaagaattg aaggcctggc
     4741 aggagaagtt tttccagaag gagcaagctc tctctgccct gcagctggag cacaccagca
     4801 cacaggccct ggtaagtgag ctgctgcctg ccaagcacct ttgtcagcag ctgcaagctg
     4861 agcaagcggc tgccgagaag cgcttccggg aggagctcga gcagagcaag caggcagctg
     4921 gtgggctcca ggcagagctg atgcgggccc agcgggagct cggagagctc ggatctttgc
     4981 ggcagaagat agttgagcag gagcgagcag cccagcagct gcgggcagag aaggccagct
     5041 atgcagagca gctgagcatg ctgaagaagg cccatggcct gctggcagag gagaaccggg
     5101 ggctgggaga gcgggccaac cttggccggc agtttctgga agtagaacta gatcaagccc
     5161 gggagaaata tgtccaggag ttggctgctg tgcgtactga tgctgagacc cacttggctg
     5221 aaatgcgaca agaagcacag agtactagcc gggagctgga ggtgatgact gccaagtacg
     5281 aaggtgccaa ggtgaaggtc ctagaggaaa ggcagaggtt ccaagaagag aggcagaaac
     5341 tcactgccca ggtggagcaa ctagaggtat ttcaaagaga gcagactaag caggtggaag
     5401 aactaagtaa gaagctgaca gagcatgacc aagccagcaa ggtgcagcaa cagaagctaa
     5461 aggctttcca ggcccagaga ggtgaaagcc agcaagaggt ccagcgcctc cagacccagc
     5521 tgaacgagct gcaggcccag ctgagccaga aggagcaggc agctgagcat tacaagctcc
     5581 agatggagaa agccaagacc cattatgatg ccaagaagca gcagaaccaa aagctgcagg
     5641 aacagcttca agacttggag gaactgcaga aggagaacaa ggagctgcgg tctgaagctg
     5701 aacgtctggg ccgtgagctg cagcaggcag ggctgaagac caaggaagct gaacagacct
     5761 gccgtcacct tacagcccag gtgcgcagcc tggaggccca ggttgcccat gcagaccagc
     5821 agcttcgtga cttgggcaaa ttccaggtgg caactgatgc cttaaagagc cgtgagcctc
     5881 aagtgaaacc ccagctggac ttaagtattg acagcctgga tctgagtttg gaggagggaa
     5941 ccccatgcag tgtggctagc aagctgcctc gtacccaacc agatggtacc agtgtccctg
     6001 gagagccagc ttcacctatc tcccagcgcc tgccccccaa agtggaatcc ctggagagcc
     6061 tctacttcac ccccactcct gctcggggtc aggccccctt ggagactagc cttgactctc
     6121 tgggggatgc cttccctgac tccggccgta agacccgctc tgctcgccgc cgtaccacac
     6181 aaatcatcaa catcactatg accaagaaat tagaattgga agagccagac agcgccaact
     6241 catccttcta cagcacgcag tctgctcctg cttcccaggc caacttgcga gccacttcct
     6301 ccactcagtc tctggcccgc ttgggttctc ctgatgatgg caactccgct ctgcttagcc
     6361 tgcctggcta ccggcctacc acacgaagct ctgctcgtcg ctcccaggcc aggatgtcca
     6421 gtggggcccc ccaagggagg aatagcttct acatgggcac ttgccaggat gagcccgagc
     6481 agctggatga ttggaaccgc atcgcagagt tgcagcagcg caaccgagta tgccccccgc
     6541 acctgaagac ctgctaccct ctggagtcca ggcctaccct gagcctggcc accatcactg
     6601 atgaggagat gaagactgga gaccccaggg aaaccttgcg tagagccagc atgcagccag
     6661 ctcagattgc tgagggtgtt ggcataacta ccaggcagca gcgcaaacga gtttcctctg
     6721 agacccacca gggccctggc actcctgagt ccaagaaagc taccagctgc tttccacgcc
     6781 ccatgacccc cagggaccgg catgaaggac gtaagcagag cagcactgct gacacccaga
     6841 agaaagcagc tccagttctc aaacaggcgg accggcgtca gtcaatggct ttcagcatcc
     6901 ttaacacacc taagaagctg ggaaatagtc ttctaaggcg aggagcttca aagaagaccc
     6961 cagcaaaggt ctcccccaat cctcgcagtg gaacccgccg ttctccacgc attgccacca
     7021 ccacaactgg cactgctact gttgccacca ctcctcgggc caagggcaag gtgaagcatg
     7081 gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg
     7141 acgatgacaa gtagtgaggg cccgatatct cgacaatcaa cctctggatt acaaaatttg
     7201 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7261 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7321 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7381 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7441 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7501 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7561 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     7621 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7681 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7741 ctccctttgg gccgcctccc cgcctggaaa cgggggaggc taactgaaac acggaaggag
     7801 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg
     7861 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     7921 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     7981 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     8041 agcagatctg cgcagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     8101 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8161 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8221 agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8281 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8341 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8401 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc
     8461 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     8521 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8581 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8641 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     8701 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8761 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     8821 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     8881 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     8941 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     9001 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     9061 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     9121 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     9181 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     9241 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     9301 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     9361 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     9421 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     9481 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     9541 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     9601 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     9661 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     9721 tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     9781 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     9841 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     9901 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9961 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
    10021 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    10081 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    10141 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    10201 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    10261 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    10321 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    10381 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    10441 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    10501 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10561 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10621 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
    10681 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10741 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10801 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10861 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
    10921 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10981 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    11041 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    11101 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    11161 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    11221 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    11281 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    11341 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    11401 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    11461 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    11521 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    11581 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11641 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11701 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    11761 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    11821 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    11881 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    11941 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    12001 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    12061 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    12121 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    12181 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
    12241 ctatggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
    12301 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
    12361 caaggcttga ccgacaattg catgaagaat ctgcttagg
//
