LOCUS       V047089                12420 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047089
VERSION     V047089
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..7100
                     /label="NUMA1(NM_006185)"
                     /note="NUMA1(NM_006185)"
                     /gene="NUMA1"
     CDS             7107..7172
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    7196..7784
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7850..7898
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8100..8528
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8542..8871
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8938..9732
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9908..10029
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10078..10094)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10102..10118
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10126..10156)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10171..10192
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10480..11068)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11239..12099)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12100..12204)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgac actccacgcc acccgggggg
      781 ctgcactcct ctcttgggtg aacagtctac acgtggctga ccctgtggag gctgtgctgc
      841 agctccagga ctgcagcatc ttcatcaaga tcattgacag aatccatggc actgaagagg
      901 gacagcaaat cttgaagcag ccggtgtcag agagactgga ctttgtgtgc agttttctgc
      961 agaaaaatcg aaaacatccc tcttccccag aatgcctggt atctgcacag aaggtgctag
     1021 agggatcaga gctggaactg gcgaagatga ccatgctgct cttataccac tctaccatga
     1081 gctccaaaag tcccagggac tgggaacagt ttgaatataa aattcaggct gagttggctg
     1141 tcattcttaa atttgtgctg gaccatgagg acgggctaaa ccttaatgag gacctagaga
     1201 acttcctaca gaaagctcct gtgccttcta cctgttctag cacattccct gaagagctct
     1261 ccccacctag ccaccaggcc aagagggaga ttcgcttcct agagctacag aaggttgcct
     1321 cctcttccag tgggaacaac tttctctcag gttctccagc ttctcccatg ggtgatatcc
     1381 tgcagacccc acagttccag atgagacggc tgaagaagca gcttgctgat gagagaagta
     1441 atagggatga gctggagctg gagctagctg agaaccgcaa gctcctcacc gagaaggatg
     1501 cacagatagc catgatgcag cagcgcattg accgcctagc cctgctgaat gagaagcagg
     1561 cggccagccc actggagccc aaggagcttg aggagctgcg tgacaagaat gagagcctta
     1621 ccatgcggct gcatgaaacc ctgaagcagt gccaggacct gaagacagag aagagccaga
     1681 tggatcgcaa aatcaaccag ctttcggagg agaatggaga cctttccttt aagctgcggg
     1741 agtttgccag tcatctgcag cagctacagg atgccctcaa tgagctgacg gaggagcaca
     1801 gcaaggccac tcaggagtgg ctagagaagc aggcccagct ggagaaggag ctcagcgcag
     1861 ccctgcagga caagaaatgc cttgaagaga agaacgaaat ccttcaggga aaactttcac
     1921 agctggaaga acacttgtcc cagctgcagg ataacccacc ccaggagaag ggcgaggtgc
     1981 tgggtgatgt cttgcagctg gaaaccttga agcaagaggc agccactctt gctgcaaaca
     2041 acacacagct ccaagccagg gtagagatgc tggagactga gcgaggccag caggaagcca
     2101 agctgcttgc tgagcggggc cacttcgaag aagaaaagca gcagctgtct agcctgatca
     2161 ctgacctgca gagctccatc tccaacctca gccaggccaa ggaagagctg gagcaggcct
     2221 cccaggctca tggggcccgg ttgactgccc aggtggcctc tctgacctct gagctcacca
     2281 cactcaatgc caccatccag caacaggatc aagaactggc tggcctgaag cagcaggcca
     2341 aagagaagca ggcccagcta gcacagaccc tccaacagca agaacaggcc tcccagggcc
     2401 tccgccacca ggtggagcag ctaagcagta gcctgaagca gaaggagcag cagttgaagg
     2461 aggtagcgga gaagcaggag gcaactaggc aggaccatgc ccagcaactg gccactgctg
     2521 cagaggagcg agaggcctcc ttaagggagc gggatgcggc tctcaagcag ctggaggcac
     2581 tggagaagga gaaggctgcc aagctggaga ttctgcagca gcaacttcag gtggctaatg
     2641 aagcccggga cagtgcccag acctcagtga cacaggccca gcgggagaag gcagagctga
     2701 gccggaaggt ggaggaactc caggcctgtg ttgagacagc ccgccaggaa cagcatgagg
     2761 cccaggccca ggttgcagag ctagagttgc agctgcggtc tgagcagcaa aaagcaactg
     2821 agaaagaaag ggtggcccag gagaaggacc agctccagga gcagctccag gccctcaaag
     2881 agtccttgaa ggtcaccaag ggcagccttg aagaggagaa gcgcagggct gcagatgccc
     2941 tggaagagca gcagcgttgt atctctgagc tgaaggcaga gacccgaagc ctggtggagc
     3001 agcataagcg ggaacgaaag gagctggaag aagagagggc tgggcgcaag gggctggagg
     3061 ctcgattaca gcagcttggg gaggcccatc aggctgagac tgaagtcctg cggcgggagc
     3121 tggcagaggc catggctgcc cagcacacag ctgagagtga gtgtgagcag ctcgtcaaag
     3181 aagtagctgc ctggcgtgag cggtatgagg atagccagca agaggaggca cagtatggcg
     3241 ccatgttcca ggaacagctg atgactttga aggaggaatg tgagaaggcc cgccaggagc
     3301 tgcaggaggc aaaggagaag gtggcaggca tagaatccca cagcgagctc cagataagcc
     3361 ggcagcagaa cgaactagct gagctccatg ccaacctggc cagagcactc cagcaggtcc
     3421 aagagaagga agtcagggcc cagaagcttg cagatgacct ctccactctg caggaaaaga
     3481 tggctgccac cagcaaagag gtggcccgct tggagacctt ggtgcgcaag gcaggtgagc
     3541 agcaggaaac agcctcccgg gagttagtca aggagcctgc gagggcagga gacagacagc
     3601 ccgagtggct ggaagagcaa cagggacgcc agttctgcag cacacaggca gcgctgcagg
     3661 ctatggagcg ggaggcagag cagatgggca atgagctgga acggctgcgg gccgcgctga
     3721 tggagagcca ggggcagcag caggaggagc gtgggcagca ggaaagggag gtggcgcggc
     3781 tgacccagga gcggggccgt gcccaggctg accttgccct ggagaaggcg gccagagcag
     3841 agcttgagat gcggctgcag aacgccctca acgagcagcg tgtggagttc gctaccctgc
     3901 aagaggcact ggctcatgcc ctgacggaaa aggaaggcaa ggaccaggag ttggccaagc
     3961 ttcgtggtct ggaggcagcc cagataaaag agctggagga acttcggcaa accgtgaagc
     4021 aactgaagga acagctggct aagaaagaaa aggagcacgc atctggctca ggagcccaat
     4081 ctgaggctgc tggcaggaca gagccaacag gccccaagct ggaggcactg cgggcagagg
     4141 tgagcaagct ggaacagcaa tgccagaagc agcaggagca ggctgacagc ctggaacgca
     4201 gcctcgaggc tgagcgggcc tcccgggctg agcgggacag tgctctggag actctgcagg
     4261 gccagttaga ggagaaggcc caggagctag ggcacagtca gagtgcctta gcctcggccc
     4321 aacgggagtt ggctgccttc cgcaccaagg tacaagacca cagcaaggct gaagatgagt
     4381 ggaaggccca ggtggcccgg ggccggcaag aggctgagag gaaaaatagc ctcatcagca
     4441 gcttggagga ggaggtgtcc atcctgaatc gccaggtcct ggagaaggag ggggagagca
     4501 aggagttgaa gcggctggtg atggccgagt cagagaagag ccagaagctg gaggagaggc
     4561 tgcgcctgct gcaggcagag acagccagca acagtgccag agctgcagaa cgcagctctg
     4621 ctctgcggga ggaggtgcag agcctccggg aggaggctga gaaacagcgg gtggcttcag
     4681 agaacctgcg gcaggagctg acctcacagg ctgagcgtgc ggaggagctg ggccaagaat
     4741 tgaaggcgtg gcaggagaag ttcttccaga aagagcaggc cctctccacc ctgcagctcg
     4801 agcacaccag cacacaggcc ctggtgagtg agctgctgcc agctaagcac ctctgccagc
     4861 agctgcaggc cgagcaggcc gctgccgaga aacgccaccg tgaggagctg gagcagagca
     4921 agcaggccgc tgggggactg cgggcagagc tgctgcgggc ccagcgggag cttggggagc
     4981 tgattcctct gcggcagaag gtggcagagc aggagcgaac agctcagcag ctgcgggcag
     5041 agaaggccag ctatgcagag cagctgagca tgctgaagaa ggcgcatggc ctgctggcag
     5101 aggagaaccg ggggctgggt gagcgggcca accttggccg gcagtttctg gaagtggagt
     5161 tggaccaggc ccgggagaag tatgtccaag agttggcagc cgtacgtgct gatgctgaga
     5221 cccgtctggc tgaggtgcag cgagaagcac agagcactgc ccgggagctg gaggtgatga
     5281 ctgccaagta tgagggtgcc aaggtcaagg tcctggagga gaggcagcgg ttccaggaag
     5341 agaggcagaa actcactgcc caggtggagc agctagaggt atttcagaga gagcaaacta
     5401 agcaggtgga agaactgagt aagaaactgg ctgactctga ccaagccagc aaggtgcagc
     5461 agcagaagct gaaggctgtc caggctcagg gaggcgagag ccagcaggag gcccagcgcc
     5521 tccaggccca gctgaatgaa ctgcaagccc agttgagcca gaaggagcag gcagctgagc
     5581 actataagct gcagatggag aaagccaaaa cacattatga tgccaagaag cagcagaacc
     5641 aagagctgca ggagcagctg cggagcctgg agcagctgca gaaggaaaac aaagagctgc
     5701 gagctgaagc tgaacggctg ggccatgagc tacagcaggc tgggctgaag accaaggagg
     5761 ctgaacagac ctgccgccac cttactgccc aggtgcgcag cctggaggca caggttgccc
     5821 atgcagacca gcagcttcga gacctgggca aattccaggt ggcaactgat gctttaaaga
     5881 gccgtgagcc ccaggctaag ccccagctgg acttgagtat tgacagcctg gatctgagct
     5941 gcgaggaggg gaccccactc agtatcacca gcaagctgcc tcgtacccag ccagacggca
     6001 ccagcgtccc tggagaacca gcctcaccta tctcccagcg cctgcccccc aaggtagaat
     6061 ccctggagag tctctacttc actcccatcc ctgctcggag tcaggccccc ctggagagca
     6121 gcctggactc cctgggagac gtcttcctgg actcgggtcg taagacccgc tccgctcgtc
     6181 ggcgcaccac gcagatcatc aacatcacca tgaccaagaa gctagatgtg gaagagccag
     6241 acagcgccaa ctcatcgttc tacagcacgc ggtctgctcc tgcttcccag gctagcctgc
     6301 gagccacctc ctctactcag tctctagctc gcctgggttc tcccgattat ggcaactcag
     6361 ccctgctcag cttgcctggc taccgcccca ccactcgcag ttctgctcgt cgttcccagg
     6421 ccggggtgtc cagtggggcc cctccaggaa ggaacagctt ctacatgggc acttgccagg
     6481 atgagcctga gcagctggat gactggaacc gcattgcaga gctgcagcag cgcaatcgag
     6541 tgtgcccccc acatctgaag acctgctatc ccctggagtc caggccttcc ctgagcctgg
     6601 gcaccatcac agatgaggag atgaaaactg gagaccccca agagaccctg cgccgagcca
     6661 gcatgcagcc aatccagata gccgagggca ctggcatcac cacccggcag cagcgcaaac
     6721 gggtctccct agagccccac cagggccctg gaactcctga gtctaagaag gccaccagct
     6781 gtttcccacg ccccatgact ccccgagacc gacatgaagg gcgcaaacag agcactactg
     6841 aggcccagaa gaaagcagct ccagcttcta ctaaacaggc tgaccggcgc cagtcgatgg
     6901 ccttcagcat cctcaacaca cccaagaagc tagggaacag ccttctgcgg cggggagcct
     6961 caaagaaggc cctgtccaag gcttccccca acactcgcag tggaacccgc cgttctccgc
     7021 gcattgccac caccacagcc agcgccgcca ctgctgccgc cattggtgcc acccctcgag
     7081 ccaagggcaa ggcaaagcac ggatccgact acaaagacca tgacggtgat tataaagatc
     7141 atgacatcga ctacaaggat gacgatgaca agtagtgagg gcccgatatc tcgacaatca
     7201 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7261 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7321 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7381 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7441 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7501 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7561 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     7621 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7681 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     7741 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa acgggggagg
     7801 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     7861 agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     7921 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     7981 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     8041 cggggcggca ggccctgcca tagcagatct gcgcagctgg ggctctaggg ggtatcccca
     8101 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8161 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8221 gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag
     8281 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8341 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8401 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     8461 agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     8521 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     8581 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     8641 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     8701 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8761 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     8821 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     8881 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     8941 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     9001 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     9061 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     9121 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     9181 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     9241 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     9301 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     9361 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     9421 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     9481 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     9541 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     9601 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     9661 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     9721 gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct
     9781 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     9841 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     9901 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     9961 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10021 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
    10081 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    10141 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    10201 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    10261 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    10321 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    10381 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    10441 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    10501 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    10561 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    10621 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
    10681 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    10741 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    10801 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    10861 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    10921 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    10981 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    11041 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    11101 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    11161 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    11221 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    11281 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    11341 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    11401 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    11461 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    11521 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    11581 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    11641 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    11701 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    11761 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    11821 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    11881 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    11941 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    12001 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    12061 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    12121 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    12181 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    12241 atcgggagat ctcccgatcc cctatggtcg actctcagta caatctgctc tgatgccgca
    12301 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    12361 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
//
