LOCUS V025438 9939 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V025438 VERSION V025438 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 678..696 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 744..1451 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 1500..4619 /label="NOD2(NM_022162)" /note="NOD2(NM_022162)" /gene="NOD2" CDS 4626..4691 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 4715..5303 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 5369..5417 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5619..6047 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 6061..6390 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 6457..7251 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 7427..7548 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(7597..7613) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7621..7637 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7645..7675) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7690..7711 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7999..8587) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8758..9618) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9619..9723) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct 661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta 721 aacttaagct tggtaccgcc accatggtga gcaagggcga ggaggataac atggccatca 781 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg 841 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 901 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg 961 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 1021 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 1081 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 1141 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg 1201 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga 1261 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc 1321 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact 1381 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg 1441 agctgtacaa gtggatcgga ggagggggaa gcggtggagg cggtagtgag ctcggatcca 1501 tgggggaaga gggtggttca gcctctcacg atgaggagga aagagcaagt gtcctcctcg 1561 gacattctcc gggttgtgaa atgtgctcgc aggaggcttt tcaggcacag aggagccagc 1621 tggtcgagct gctggtctca gggtccctgg aaggcttcga gagtgtcctg gactggctgc 1681 tgtcctggga ggtcctctcc tgggaggact acgagggctt ccacctcctg ggccagcctc 1741 tctcccactt ggccaggcgc cttctggaca ccgtctggaa taagggtact tgggcctgtc 1801 agaagctcat cgcggctgcc caagaagccc aggccgacag ccagtccccc aagctgcatg 1861 gctgctggga cccccactcg ctccacccag cccgagacct gcagagtcac cggccagcca 1921 ttgtcaggag gctccacagc catgtggaga acatgctgga cctggcatgg gagcggggtt 1981 tcgtcagcca gtatgaatgt gatgaaatca ggttgccgat cttcacaccg tcccagaggg 2041 caagaaggct gcttgatctt gccacggtga aagcgaatgg attggctgcc ttccttctac 2101 aacatgttca ggaattacca gtcccattgg ccctgccttt ggaagctgcc acatgcaaga 2161 agtatatggc caagctgagg accacggtgt ctgctcagtc tcgcttcctc agtacctatg 2221 atggagcaga gacgctctgc ctggaggaca tatacacaga gaatgtcctg gaggtctggg 2281 cagatgtggg catggctgga cccccgcaga agagcccagc caccctgggc ctggaggagc 2341 tcttcagcac ccctggccac ctcaatgacg atgcggacac tgtgctggtg gtgggtgagg 2401 cgggcagtgg caagagcacg ctcctgcagc ggctgcactt gctgtgggct gcagggcaag 2461 acttccagga atttctcttt gtcttcccat tcagctgccg gcagctgcag tgcatggcca 2521 aaccactctc tgtgcggact ctactctttg agcactgctg ttggcctgat gttggtcaag 2581 aagacatctt ccagttactc cttgaccacc ctgaccgtgt cctgttaacc tttgatggct 2641 ttgacgagtt caagttcagg ttcacggatc gtgaacgcca ctgctccccg accgacccca 2701 cctctgtcca gaccctgctc ttcaaccttc tgcagggcaa cctgctgaag aatgcccgca 2761 aggtggtgac cagccgtccg gccgctgtgt cggcgttcct caggaagtac atccgcaccg 2821 agttcaacct caagggcttc tctgaacagg gcatcgagct gtacctgagg aagcgccatc 2881 atgagcccgg ggtggcggac cgcctcatcc gcctgctcca agagacctca gccctgcacg 2941 gtttgtgcca cctgcctgtc ttctcatgga tggtgtccaa atgccaccag gaactgttgc 3001 tgcaggaggg ggggtcccca aagaccacta cagatatgta cctgctgatt ctgcagcatt 3061 ttctgctgca tgccaccccc ccagactcag cttcccaagg tctgggaccc agtcttcttc 3121 ggggccgcct ccccaccctc ctgcacctgg gcagactggc tctgtggggc ctgggcatgt 3181 gctgctacgt gttctcagcc cagcagctcc aggcagcaca ggtcagccct gatgacattt 3241 ctcttggctt cctggtgcgt gccaaaggtg tcgtgccagg gagtacggcg cccctggaat 3301 tccttcacat cactttccag tgcttctttg ccgcgttcta cctggcactc agtgctgatg 3361 tgccaccagc tttgctcaga cacctcttca attgtggcag gccaggcaac tcaccaatgg 3421 ccaggctcct gcccacgatg tgcatccagg cctcggaggg aaaggacagc agcgtggcag 3481 ctttgctgca gaaggccgag ccgcacaacc ttcagatcac agcagccttc ctggcagggc 3541 tgttgtcccg ggagcactgg ggcctgctgg ctgagtgcca gacatctgag aaggccctgc 3601 tccggcgcca ggcctgtgcc cgctggtgtc tggcccgcag cctccgcaag cacttccact 3661 ccatcccgcc agctgcaccg ggtgaggcca agagcgtgca tgccatgccc gggttcatct 3721 ggctcatccg gagcctgtac gagatgcagg aggagcggct ggctcggaag gctgcacgtg 3781 gcctgaatgt tgggcacctc aagttgacat tttgcagtgt gggccccact gagtgtgctg 3841 ccctggcctt tgtgctgcag cacctccggc ggcccgtggc cctgcagctg gactacaact 3901 ctgtgggtga cattggcgtg gagcagctgc tgccttgcct tggtgtctgc aaggctctgt 3961 atttgcgcga taacaatatc tcagaccgag gcatctgcaa gctcattgaa tgtgctcttc 4021 actgcgagca attgcagaag ttagctctat tcaacaacaa attgactgac ggctgtgcac 4081 actccatggc taagctcctt gcatgcaggc agaacttctt ggcattgagg ctggggaata 4141 actacatcac tgccgcggga gcccaagtgc tggccgaggg gctccgaggc aacacctcct 4201 tgcagttcct gggattctgg ggcaacagag tgggtgacga gggggcccag gccctggctg 4261 aagccttggg tgatcaccag agcttgaggt ggctcagcct ggtggggaac aacattggca 4321 gtgtgggtgc ccaagccttg gcactgatgc tggcaaagaa cgtcatgcta gaagaactct 4381 gcctggagga gaaccatctc caggatgaag gtgtatgttc tctcgcagaa ggactgaaga 4441 aaaattcaag tttgaaaatc ctgaagttgt ccaataactg catcacctac ctaggggcag 4501 aagccctcct gcaggccctt gaaaggaatg acaccatcct ggaagtctgg ctccgaggga 4561 acactttctc tctagaggag gttgacaagc tcggctgcag ggacaccaga ctcttgcttc 4621 tcgaggacta caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg 4681 acgatgacaa gtagtgaggg cccgatatct cgacaatcaa cctctggatt acaaaatttg 4741 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 4801 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 4861 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 4921 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 4981 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 5041 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 5101 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 5161 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 5221 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 5281 ctccctttgg gccgcctccc cgcctggaaa cgggggaggc taactgaaac acggaaggag 5341 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg 5401 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac 5461 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc 5521 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat 5581 agcagatctg cgcagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 5641 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 5701 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 5761 agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 5821 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 5881 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 5941 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc 6001 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 6061 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 6121 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 6181 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 6241 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 6301 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 6361 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 6421 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 6481 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 6541 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 6601 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg 6661 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 6721 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 6781 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 6841 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 6901 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 6961 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat 7021 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 7081 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 7141 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 7201 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 7261 tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 7321 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 7381 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 7441 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 7501 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 7561 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 7621 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 7681 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 7741 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 7801 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 7861 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 7921 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 7981 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 8041 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 8101 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 8161 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 8221 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 8281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 8341 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 8401 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 8461 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 8521 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 8581 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 8641 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 8701 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 8761 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 8821 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 8881 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 8941 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 9001 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 9061 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 9121 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 9181 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 9241 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 9301 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 9361 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 9421 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 9481 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 9541 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 9601 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 9661 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 9721 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc 9781 ctatggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc 9841 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg 9901 caaggcttga ccgacaattg catgaagaat ctgcttagg //