LOCUS V025798 7608 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V025798 VERSION V025798 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 744..770 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 774..800 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 804..830 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 840..881 /codon_start=1 /product="epitope tag from simian virus 5" /transl_table=1 /translation="GKPIPNPLLGLDST" /label="V5 tag" CDS 888..2279 /label="NDUFV1(NM_007103)" /note="NDUFV1(NM_007103)" /gene="NDUFV1" misc_feature 2384..2972 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3038..3086 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3288..3716 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3730..4059 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4126..4920 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5096..5217 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5266..5282) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5290..5306 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5314..5344) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5359..5380 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5668..6256) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6427..7287) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7288..7392) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagc tagcgccacc atgtacccat acgacgtacc tgattatgca acttatcctt 781 atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca ggcggaggtg 841 gtaagcctat ccctaaccct ctcctcggtc tcgattctac aggatccatg ctggcaacac 901 ggcggctgct cggctggtcg cttcccgcgc gggtatctgt gcgtttcagc ggcgacacga 961 cagcacccaa gaaaacctca tttggctcgc tgaaggatga agaccggatt ttcaccaacc 1021 tgtacggccg ccatgactgg aggctgaaag gttccctgag tcgaggtgac tggtacaaga 1081 caaaggagat cctgctgaag gggcccgact ggatcctggg cgagatcaag acatcgggtt 1141 tgaggggccg tggaggcgct ggcttcccca ctggcctcaa gtggagcttc atgaataagc 1201 cctcagatgg caggcccaag tatctggtgg tgaacgcaga cgagggggag ccgggcacct 1261 gcaaggaccg ggagatctta cgccatgatc ctcacaagct gctggaaggc tgcctggtgg 1321 ggggccgggc catgggcgcc cgcgctgcct atatctacat ccgaggggaa ttctacaatg 1381 aggcctccaa tctgcaggtg gccatccgag aggcctatga ggcaggtctg attggcaaga 1441 atgcttgtgg ctctggctat gattttgacg tgtttgtggt gcgcggggct ggggcctaca 1501 tctgtggaga ggagacagcg ctcatcgagt ccattgaggg caagcagggc aagccccgcc 1561 tgaagccccc cttccccgca gacgtgggag tgtttggctg ccccacaact gtggccaacg 1621 tggagacagt ggcagtgtcc cccacaatct gccgccgtgg aggtacctgg tttgctggct 1681 ttggcagaga acgcaactca ggcaccaaac tattcaacat ctctggccat gtcaaccacc 1741 cttgcactgt ggaggaggag atgtctgtgc ccttgaaaga actgattgag aagcatgctg 1801 ggggtgtcac gggcggctgg gacaacctcc ttgctgtgat ccctggcggc tcgtctaccc 1861 cactgatccc caagtctgtg tgtgagacgg tgctgatgga cttcgatgcg ctggtgcagg 1921 cacagacagg cctgggcaca gctgcggtga tcgtcatgga ccgctcgacg gacatcgtga 1981 aagccatcgc ccgcctcatt gagttctata agcacgagag ctgtggccag tgtaccccat 2041 gccgtgaggg tgtggactgg atgaacaagg tgatggcacg tttcgtgagg ggggatgccc 2101 ggccggccga gatcgactcc ctgtgggaga tcagcaagca gatagaaggc catacgattt 2161 gtgctctggg tgacggggcc gcctggcctg tgcagggtct gatccgccac tttcggccgg 2221 agctcgagga gcggatgcag cggtttgccc agcagcatca ggcccggcag gctgcctctt 2281 agtagaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag ggccccttaa 2341 gggttcgatc cctaccggtt agtaatgagt ttgatatctc gacaatcaac ctctggatta 2401 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 2461 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 2521 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 2581 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 2641 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 2701 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 2761 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg 2821 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 2881 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 2941 gagtcggatc tccctttggg ccgcctcccc gcctggaaac gggggaggct aactgaaaca 3001 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 3061 acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc 3121 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc 3181 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg 3241 ccctgccata gcagatctgc gcagctgggg ctctaggggg tatccccacg cgccctgtag 3301 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 3361 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 3421 tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca 3481 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 3541 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 3601 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg 3661 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 3721 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 3781 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 3841 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 3901 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 3961 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 4021 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 4081 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 4141 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 4201 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 4261 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 4321 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 4381 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 4441 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 4501 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 4561 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 4621 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 4681 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 4741 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 4801 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 4861 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 4921 gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 4981 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 5041 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 5101 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 5161 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 5221 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 5281 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 5341 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 5401 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 5461 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 5521 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 5581 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 5641 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 5701 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 5761 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 5821 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta 5881 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 5941 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 6001 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 6061 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 6121 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 6181 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 6241 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 6301 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 6361 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 6421 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 6481 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 6541 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 6601 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 6661 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 6721 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 6781 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 6841 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 6901 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 6961 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 7021 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 7081 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 7141 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 7201 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 7261 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 7321 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 7381 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct 7441 cccgatcccc tatggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag 7501 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct 7561 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagg //