LOCUS       V028315                13369 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028315
VERSION     V028315
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..8025
                     /label="NCOR1(NM_006311)"
                     /note="NCOR1(NM_006311)"
                     /gene="NCOR1"
     CDS             8032..8121
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    8145..8733
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    8799..8847
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      9049..9477
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9491..9820
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             9887..10681
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    10857..10978
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(11027..11043)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11051..11067
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11075..11105)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11120..11141
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11429..12017)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12188..13048)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13049..13153)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtc aagttcaggt
      721 tatcctccca accaaggagc attcagcaca gaacaaagtc gttatcctcc tcactctgtc
      781 cagtatacat ttcccaacac ccgccaccag caggagttcg cagtccctga ttatcgttcc
      841 tctcatcttg aagtgagtca ggcatcacag cttttgcagc aacagcagca gcaacagctt
      901 cgaaggcgac cttccttgct ttcagaattt cacccaggtt ctgacaggcc tcaagaaagg
      961 agaactagtt atgaaccgtt tcatccaggc ccatccccag tggatcatga ttcactggaa
     1021 tcgaagcgac cacgtctgga acaggtttct gattctcatt ttcagcgtgt cagtgctgcg
     1081 gttttgcctt tagtgcaccc gctgccagaa gggctgaggg cttctgcaga tgctaagaag
     1141 gatccagcat tcggaggcaa acatgaagct ccatcctctc caatttcggg gcaaccatgt
     1201 ggagatgatc aaaatgcttc accttcaaaa ctctcaaagg aagagttaat acagagtatg
     1261 gatcgtgtag atcgagaaat tgcaaaagta gaacagcaga tccttaaact gaaaaagaaa
     1321 caacaacagc ttgaagaaga ggcagctaaa cctcctgagc ctgagaagcc cgtgtcccct
     1381 cctcctgtgg agcagaaaca ccgcagtatt gtccaaatta tttatgatga gaatcggaaa
     1441 aaagcagaag aagctcataa aatttttgaa ggtcttggcc caaaagttga actgccactg
     1501 tataaccagc catcagatac caaggtgtac catgagaaca tcaagacaaa ccaggtgatg
     1561 aggaaaaaac tcattttatt ttttaaaaga agaaatcatg caagaaaaca aagggaacaa
     1621 aaaatctgcc agcgttatga tcagctcatg gaggcatggg agaaaaaagt ggacagaata
     1681 gaaaataatc ctcggaggaa agctaaagaa agcaaaacaa gggaatacta tgaaaagcag
     1741 tttccagaaa ttcgaaaaca aagagaacag caagaaagat ttcagcgagt tgggcagagg
     1801 ggagctggtc tttcagccac cattgctagg agtgagcatg agatttctga aattattgat
     1861 gggctctctg agcaggagaa taatgagaaa caaatgcggc agctctctgt gattccacct
     1921 atgatgtttg atgcagaaca aagacgagtc aagttcatta acatgaatgg gcttatggag
     1981 gaccctatga aagtgtataa agataggcag tttatgaatg tttggactga ccatgaaaag
     2041 gagatcttta aggacaagtt tatccagcat ccaaaaaact ttggactaat tgcatcatac
     2101 ttggagagga agagtgttcc tgattgtgtt ttgtattact atttaaccaa gaaaaatgag
     2161 aattataaag ccctcgtcag aaggaattat gggaaacgca gaggcagaaa ccagcaaatt
     2221 gctcgaccct cgcaagaaga aaaagtagaa gaaaaagaag aggataaagc agaaaaaaca
     2281 gaaaaaaaag aagaagaaaa gaaagatgaa gaggaaaaag atgaaaaaga agactccaaa
     2341 gaaaatacca aggaaaagga caagatagat ggtacagcag aagaaactga ggaaagagag
     2401 caagccacac cccgggggcg aaagactgcc aacagtcagg gccgccgtaa gggccggatc
     2461 accaggtcca tgacaaacga agctgcagct gccagtgctg cagccgcagc ggctactgaa
     2521 gagcccccac cacctctgcc accgccacca gaacccattt ctacagagcc tgtggagacc
     2581 tctcgatgga cagaagaaga aatggaagtt gctaaaaaag gtctagtaga acatggtcgt
     2641 aactgggcag caattgctaa aatggtggga acgaaaagtg aagctcaatg taaaaacttc
     2701 tattttaact ataaaaggcg acacaatctt gacaacctct tacagcagca taaacagaaa
     2761 acttcacgaa aacctcgtga agagcgagat gtgtctcaat gtgaaagtgt cgcttccact
     2821 gtttctgctc aggaggatga agatattgaa gcctccaatg aagaagaaaa tccagaagac
     2881 agcgaagttg aagctgtcaa gcccagcgag gacagtcctg aaaatgctac ttctcgagga
     2941 aacacagaac ctgcggttga gcttgagccc accacggaaa ctgcacccag tacatctccc
     3001 tccttagcag ttccaagtac aaaaccagct gaagatgaaa gtgtggagac ccaggtgaat
     3061 gacagcatca gtgctgagac agcagagcag atggatgtag atcagcagga gcacagtgct
     3121 gaagagggtt ctgtttgtga tcccccaccc gctaccaaag ctgactctgt ggacgttgaa
     3181 gtgagggtgc cagaaaacca tgcatctaaa gttgaaggtg ataataccaa agaaagagac
     3241 ttggatagag ccagtgagaa ggtggaacct agagatgaag atttggtggt agctcagcaa
     3301 ataaatgccc aaaggcccga gccccagtca gacaatgatt ccagtgccac gtgcagcgct
     3361 gatgaggatg tggatggaga gccagagagg cagagaatgt ttcctatgga ctcaaagcct
     3421 tcactgttaa accccactgg atctatactc gtctcatctc cgttaaaacc aaatccactg
     3481 gatctgccac agcttcagca tcgagctgct gttatcccac caatggtatc ctgcacccca
     3541 tgtaacatac caattggaac cccagtgagc ggctatgctc tctaccagcg acacattaaa
     3601 gcaatgcatg agtcagcact cctggaggag cagcggcaga gacaagaaca gatagatttg
     3661 gaatgtagaa gttctacaag tccatgtggc acatccaaga gtccaaacag agagtgggaa
     3721 gtccttcagc ctgctccaca tcaagtgata actaatctcc ctgaaggcgt tcggcttccg
     3781 acaactcgac caaccaggcc accgccccct ctcatcccgt catccaaaac cacagtggct
     3841 tcagaaaaac catcttttat aatgggaggc tccatctcac agggaacacc aggcacttat
     3901 ttgacttctc ataatcaggc ttcctacact caagaaacac ccaagccgtc agtgggatct
     3961 atctctcttg gactgccacg gcaacaggaa tctgccaaat cagctacttt gccctacatc
     4021 aagcaggaag aattttctcc ccgaagccaa aactcacaac ctgagggtct gttggtcagg
     4081 gcccaacatg aaggtgtagt cagaggtacc gcaggagcca tacaagaagg aagtataact
     4141 cggggaactc caaccagcaa aatttcagtg gagagcattc catccctacg gggctctatc
     4201 actcagggca ccccggctct gccccagact ggcataccaa cagaggcttt ggtgaagggg
     4261 tccatttcga gaatgcccat tgaagacagc agtcctgaga aaggcagaga ggaagctgca
     4321 tccaaaggcc atgttattta tgaaggcaaa agtggacata tcttgtcata tgataatatt
     4381 aagaatgccc gagaagggac taggagtcca agaacagctc atgaaatcag tttaaagaga
     4441 agctatgaat cagtggaagg aaatataaag caagggatgt caatgaggga gtctcctgta
     4501 tcagcaccgt tagaggggct gatatgccga gcattaccca gggggagtcc tcattctgac
     4561 ctcaaagaaa ggactgtatt gtctggctcc ataatgcagg ggacaccaag agcaacaact
     4621 gaaagctttg aagatggcct taaatatccc aaacaaatta aaagggaaag tcctcccata
     4681 cgagcatttg aaggtgccat taccaaagga aaaccatatg atggcatcac caccatcaaa
     4741 gaaatggggc gttccattca tgagattcca aggcaagata ttttaactca ggaaagtcgg
     4801 aaaactccag aagtggtcca gagcacacgg ccgataattg agggttccat ttcccagggc
     4861 acaccaataa agtttgacaa caactcaggt caatctgcca tcaaacacaa tgtcaaatcc
     4921 ttaatcacgg ggcctagcaa actatcccgt ggaatgcctc cgctggaaat tgtgccagag
     4981 aacataaaag tggtagaacg gggaaaatat gaggatgtga aagcaggcga gaccgtgcgt
     5041 tcccggcaca cgtcagtggt aagctctggc ccctccgttc ttaggtccac actgcatgaa
     5101 gctcccaaag cacaactgag ccctgggatt tatgatgaca ccagtgcacg gaggacccct
     5161 gtgagttatc aaaacaccat gtccagaggc tcacccatga tgaacagaac ttctgatgtt
     5221 acaatttctt ctaacaagtc taccaatcat gaaaggaaat cgacactgac ccctacccag
     5281 agggaaagta tcccagcgaa gtctccagtg cctggggtgg accctgtcgt gagccacagt
     5341 ccgtttgatc cccatcacag aggcagcact gcaggcgagg tttatcggag ccacctgccc
     5401 acgcacttgg atccagccat gccttttcac agggctttgg atcctgcagc ggctgcttac
     5461 ctgtttcaga gacagctttc accaactcca ggttacccaa gtcagtatca gctttacgca
     5521 atggagaaca caagacagac aatcttaaat gattacatta cctcacaaca gatgcaagtg
     5581 aacttgcgtc cagatgtggc cagaggactc tccccaagag agcagccact gggtctccca
     5641 tacccagcaa cgagaggaat cattgacctg accaatatgc ctccaacaat tttagtgcct
     5701 catccagggg gaacaagcac tcctcccatg gacagaatca cttatattcc tggtacacag
     5761 attactttcc ctcccaggcc gtacaactct gcttccatgt ctccaggaca cccaacacac
     5821 cttgcagctg ctgcaagtgc tgagagggaa cgggaacggg agcgggagaa ggagcgggag
     5881 cgggaacgga ttgctgcagc ttcctccgac ctctacctgc ggccaggctc agaacagcct
     5941 ggccgacctg gcagtcatgg atatgttcgc tccccttccc cttcagtaag aactcaggag
     6001 accatgttgc aacagagacc cagtgttttc caaggaacca atggaaccag tgtaatcaca
     6061 cctttggatc caactgctca gctacgaatc atgccactgc ctgctggggg cccttcaata
     6121 agccaaggcc tgccagcctc ccgttacaac actgctgcgg atgccctggc tgctcttgtg
     6181 gatgctgcag cttctgcacc ccagatggat gtgtccaaaa caaaagagag taagcatgaa
     6241 gctgccaggt tagaagaaaa tttgagaagc aggtcagcag cagttagtga acagcagcag
     6301 ctagagcaga aaaccctgga ggtggagaag agatctgttc agtgtttata cacttcttca
     6361 gcctttccaa gtggcaagcc ccagcctcat tcttcagtag tttattctga ggctgggaaa
     6421 gataaagggc ctcctccaaa atccagatat gaggaagagc taaggaccag agggaagact
     6481 accattactg cagctaactt catagacgtg atcatcaccc ggcaaattgc ctcggacaag
     6541 gatgcgaggg aacgtggctc tcaaagttca gactcttcta gtagcttatc ttctcacagg
     6601 tatgaaacac ctagcgatgc tattgaggtg ataagtcctg ccagctcacc tgcgccaccc
     6661 caggagaaac tgcagaccta tcagccagag gttgttaagg caaatcaagc ggaaaatgat
     6721 cctaccagac aatatgaagg accattacat cactatcgac cacagcagga atcaccatct
     6781 ccccaacaac agctgccccc ttcttcacag gcagagggaa tggggcaagt gcccaggacc
     6841 catcggctga tcacacttgc tgatcacatc tgtcaaatta tcacacaaga ttttgctaga
     6901 aatcaagttt cctcgcagac tccccagcag cctcctactt ctacattcca gaactcacct
     6961 tctgctttgg tatctacacc tgtgaggact aaaacatcaa accgttacag cccagaatcc
     7021 caggctcagt ctgtccatca tcaaagacca ggttcaaggg tctctccaga aaatcttgtg
     7081 gacaaatcca ggggaagtag gcctggaaaa tccccagaga ggagtcacgt ctcttcggag
     7141 ccctacgagc ccatctcccc accccaggtt ccggttgtgc atgagaaaca ggacagcttg
     7201 ctgctcttgt ctcagagggg cgcagagcct gcagagcaga ggaatgatgc ccgctcacca
     7261 gggagtataa gctacttgcc ttcattcttc accaagcttg aaaatacatc acccatggtt
     7321 aaatcaaaga agcaggagat ttttcgtaag ttgaactcct ctggtggagg tgactctgat
     7381 atggcagctg ctcagccagg aactgagatc tttaatctgc cagcagttac tacgtcaggc
     7441 tcagttagct ctagaggcca ttcttttgct gatcctgcca gtaatcttgg gctggaagac
     7501 attatcagga aggctctcat gggaagcttt gatgacaaag ttgaggatca tggagttgtc
     7561 atgtcccagc ctatgggagt agtgcctggt actgccaaca cctcagttgt gaccagtggt
     7621 gagacacgaa gagaggaagg ggacccatca cctcattcag gaggagtttg caaaccaaag
     7681 ctgatcagca agtcaaacag caggaaatct aagtctccta tacctgggca aggctactta
     7741 ggaacggaac ggccctcttc agtctcctct gtacattcag aaggggatta ccataggcag
     7801 acgccagggt gggcctggga agacaggccc tcttcaacag gctcaactca gtttccttat
     7861 aaccctctga ctatgcggat gctcagcagt actccaccaa caccgattgc atgtgctccc
     7921 tctgcggtga accaagcagc tcctcaccaa cagaacagga tctgggagcg agagcctgcc
     7981 ccactgctct cagcacagta cgagaccctg tcggatagtg atgacctcga ggaacaaaaa
     8041 ctaatatcag aggaagattt ggaacagaaa ctaatcagtg aagaagattt agagcaaaag
     8101 ttaatttccg aggaggactt atagtgaggg cccgatatct cgacaatcaa cctctggatt
     8161 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     8221 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     8281 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     8341 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     8401 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     8461 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     8521 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     8581 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     8641 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     8701 cgagtcggat ctccctttgg gccgcctccc cgcctggaaa cgggggaggc taactgaaac
     8761 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     8821 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     8881 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     8941 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     9001 gccctgccat agcagatctg cgcagctggg gctctagggg gtatccccac gcgccctgta
     9061 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     9121 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     9181 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc
     9241 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     9301 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     9361 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg
     9421 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     9481 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     9541 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     9601 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     9661 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9721 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     9781 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     9841 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     9901 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     9961 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
    10021 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
    10081 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
    10141 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
    10201 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
    10261 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
    10321 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
    10381 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
    10441 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
    10501 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
    10561 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
    10621 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
    10681 agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
    10741 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
    10801 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
    10861 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    10921 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    10981 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    11041 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    11101 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    11161 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    11221 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    11281 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    11341 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    11401 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    11461 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    11521 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    11581 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
    11641 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    11701 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    11761 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    11821 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
    11881 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    11941 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    12001 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    12061 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    12121 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    12181 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    12241 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    12301 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    12361 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    12421 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    12481 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    12541 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    12601 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    12661 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    12721 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    12781 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    12841 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    12901 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    12961 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    13021 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    13081 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    13141 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    13201 tcccgatccc ctatggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca
    13261 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
    13321 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttagg
//
