LOCUS V035789 7384 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V035789 VERSION V035789 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..2043 /label="NADK(NM_023018)" /note="NADK(NM_023018)" /gene="NADK" CDS 2050..2076 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2080..2106 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2110..2136 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 2160..2748 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2814..2862 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3064..3492 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3506..3835 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3902..4696 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4872..4993 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5042..5058) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5066..5082 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5090..5120) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5135..5156 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5444..6032) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6203..7063) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7064..7168) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgga aatggaacaa 721 gaaaaaatga ccatgaataa ggaattgagt ccagacgcgg ctgcttactg ctgctcggcc 781 tgccacggcg atgagacctg gagttacaac caccccatcc ggggccgggc caagtctcgc 841 agcctgtctg cctcgcccgc cctggggagc accaaggagt tcaggaggac acgctctctt 901 catgggccat gcccggtgac cacttttgga ccaaaggcct gtgtgctgca gaacccccag 961 accatcatgc acattcagga ccccgcgagc cagcggctga cgtggaacaa gtccccaaag 1021 agcgtccttg tcatcaagaa gatgagagat gccagcctac tgcagccgtt caaggagctc 1081 tgcacgcacc tcatggagga gaacatgatc gtgtatgtgg aaaagaaagt gctagaagac 1141 cctgccatcg ccagcgatga aagctttggg gcagtgaaga agaaattctg tacctttcga 1201 gaagattatg atgacatttc caatcagata gacttcatca tctgcctggg gggagacggg 1261 acgctgctgt acgcttcctc gcttttccag ggcagcgtcc ctccggtcat ggccttccac 1321 ctgggctccc tgggcttcct gaccccattc agctttgaga actttcagtc ccaagttact 1381 caggtgatag aggggaacgc agctgttgtt ctccggagtc ggctgaaggt cagggtggtg 1441 aaggagctcc gggggaagaa gacggccgtg cacaatgggc tgggtgagaa cggctcgcag 1501 gctgcaggcc tggacatgga tgtcgggaag caggccatgc agtaccaggt cctgaatgag 1561 gtggtgattg acagaggccc ctcctcctac ctgtccaatg tggatgtcta cctggacgga 1621 cacctcatca ccacggtgca gggcgacgga gtgatcgtgt ccaccccgac gggcagcacg 1681 gcgtatgcgg ccgcggccgg ggcctccatg atccacccca acgtgccggc catcatgatc 1741 acgcccatct gcccccactc gctgtccttc cggcccatcg tggtccccgc aggggtcgag 1801 ctgaagatca tgctgtcacc tgaagcaagg aacacagcat gggtgtcctt tgatggacgg 1861 aagagacaag agatccgcca tggagacagc atcagcatca ctacctcatg ctacccgctc 1921 ccctccatct gtgtgcggga ccccgtgagc gactggtttg agagcctcgc ccagtgcctg 1981 cattggaacg tccggaagaa gcaagcccac ttcgaggagg aggaggagga ggaggaggag 2041 ggcctcgagt acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat 2101 tatgctagct atccttatga tgtcccagac tacgcatagt gagggcccga tatctcgaca 2161 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 2221 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 2281 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 2341 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 2401 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 2461 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 2521 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 2581 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 2641 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 2701 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaaacgggg 2761 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat 2821 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt 2881 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg 2941 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca 3001 acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc 3061 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 3121 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 3181 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat 3241 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 3301 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 3361 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 3421 tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 3481 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 3541 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 3601 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 3661 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 3721 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 3781 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 3841 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 3901 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 3961 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 4021 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 4081 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 4141 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 4201 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 4261 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 4321 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 4381 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 4441 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 4501 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 4561 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 4621 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 4681 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca 4741 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 4801 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 4861 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 4921 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 4981 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 5041 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 5101 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 5161 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 5221 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 5281 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 5341 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 5401 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 5461 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 5521 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 5581 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat 5641 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 5701 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 5761 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 5821 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 5881 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 5941 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 6001 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 6061 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 6121 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 6181 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 6241 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 6301 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 6361 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 6421 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 6481 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 6541 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 6601 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 6661 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 6721 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 6781 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 6841 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 6901 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 6961 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 7021 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 7081 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 7141 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 7201 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc 7261 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc 7321 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct 7381 tagg //