LOCUS       V027289                11613 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027289
VERSION     V027289
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..6293
                     /label="Myo5b(NM_017083)"
                     /note="Myo5b(NM_017083)"
                     /gene="Myo5b"
     CDS             6300..6365
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    6389..6977
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7043..7091
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7293..7721
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7735..8064
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8131..8925
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9101..9222
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9271..9287)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9295..9311
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9319..9349)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9364..9385
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9673..10261)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10432..11292)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11293..11397)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgtc gtacagcgag ctctacagcc
      781 ggtacacgag ggtctggatc cccgacccag atgaggtgtg gcgctcggct gaactaacca
      841 aggactacaa agatggggat gagagcctac aactcagact ggaagatgac acaattctgg
      901 attacccaat cgatgtccaa aataaccagg tgccattctt gcggaatcca gatatcttag
      961 taggagaaaa cgacctcact gcactcagcc atctccacga gcccgcagtt ctgcataact
     1021 taaaggtccg tttcctggag tctaaccaca tctacactta ctgtggaatt gtgcttgttg
     1081 ccattaatcc ctatgagcag ctgccaatct acgggcagga tgtcatctat gcctacagtg
     1141 gccaaaatat gggcgacatg gatccccaca tctttgctgt agcagaagaa gcctacaagc
     1201 agatggccag agatgaaaag aaccaatcca tcatagtcag cggagagtct ggagcaggga
     1261 agaccgtgtc agccaagtat gccatgcgct attttgccac agttgggggc tcagccagtg
     1321 ataccaacat cgaagagaag gtcctggctt ccagtcccat catggaggcc atcgggaatg
     1381 ccaagacaac tcgcaacgac aatagcagcc gatttgggaa gtacattgag atcggctttg
     1441 ataaaaagta ccacatcatc ggggccaaca tgaggaccta tttgctggag aagtccaggg
     1501 tggtctttca ggcggatgat gagaggaact accacatctt ttaccagctc tgtgctgcgg
     1561 ccagccttcc tgagtttaag gagctcgcac taacatgtgc agaggacttt ttctacaccg
     1621 cccatggagg aaacacgacc atcgagggtg tagatgatgc agaggacttt gagaagacaa
     1681 gacaagccct cacactcctt ggagttcggg agtcccatca aatcagcatc tttaagataa
     1741 ttgcttctat cttgcacctt ggaagtgtgg agatccaggc cgagcgggat ggggactcct
     1801 gcagcatatc gccccaggac gaacacctga gcaacttctg ccgcctgcta ggaatagagc
     1861 acagtcagat ggagcactgg ctgtgtcatc ggaagctggt caccacctcc gagacctacg
     1921 tcaagaccat gtccctgcag caagtggtca acgcacgcaa tgccttggcc aagcacatct
     1981 atgcccagct gttctcctgg attgtggagc acatcaacaa ggccctgcaa acctccctta
     2041 aacaacactc cttcatcggg gtcctggata tttatgggtt tgagaccttc gagattaata
     2101 gcttcgagca gttttgtatc aactatgcca acgaaaagct ccagcaacag ttcaactcgc
     2161 atgtgttcaa gctggagcaa gaagagtata tgaaggagca gatcccgtgg accttgattg
     2221 acttctatga taaccaaccg tgcatagacc tcatagaagc caagctgggt atcctggacc
     2281 tgttggatga ggagtgtaag gtccccaaag gaactgatca gaactgggct cagaaactct
     2341 atgaacggca ctccaacagt caacacttcc agaaaccacg catgtccaac acggccttca
     2401 ttgtcatcca ctttgcagac aaggtggaat acctttcaga tggttttctg gagaaaaaca
     2461 gggacacggt atatgaagaa cagatcaaca tcctgaaagc cagcaagttt ccactagtgg
     2521 ctgatttgtt ccgtgatgac aaggactctg ttcctgccac caacacagct aagagtcggt
     2581 catcttcaaa gatcaatgtt cgttcttcca gacccctcat gaaggccccc aacaaggagc
     2641 acaagaaatc cgtgggctac cagttccgca cttccctaaa cctgcttatg gagactctga
     2701 atgccacaac gccacactac gttcgatgca tcaagcccaa cgacgaaaag ctccccttcc
     2761 acttcgaccc aaagagagct gtgcagcagc tcagagcctg cggcgtgttg gagaccattc
     2821 ggatcagcgc ggcaggctac ccgtccaggt ggacctacca tgacttcttc aaccggtatc
     2881 gggtgttgat gaagaagaga gagcttgcca acaccaccga caagaagaat atctgcaagt
     2941 ctgtcctgga gagtctcatc aaggatccag acaagttcca gtttggccgc accaagatct
     3001 tcttccgggc aggtcaggtg gcctacctgg agaagcttcg ggcagacaag ttccgggagg
     3061 ccaccatcat gatccaaaag acggtcaggg gctggctgca gagagtgaag taccggaggc
     3121 tgagagcagc tacgctaacc ctgcagagat tctgccgagg atacttagcc cgcagactga
     3181 ctgagcactt gaggagaacc cgggcggcca tagtgttcca gaagcagtac cgcatgctga
     3241 aggcccgccg agcctactgc agggtccgca gggctgcggt catcatccag tcctacacga
     3301 gggccatgtg tgtacgcaga agctaccgcc aggtcctcac ggagcacaaa gccaccatca
     3361 tccagaagta cgcccggggc tggatggcac ggagacattt tcagcggcag cgggatgcag
     3421 ccattgtcat ccagtgtgcc ttccggaggc tcaaggccag gcaggcactg aaggccctca
     3481 agatcgaggc ccgttctgca gagcatctga aacgcctcaa cgtgggcatg gagaacaaag
     3541 ttgtccagct gcagcggaag attgatgacc agaacaaaga gttcaagact ctgtcagagc
     3601 agttgtctgc agttacctcc acccatgcca tggaggtgga gaagctgaag aaggagctgg
     3661 cgcgttacca gcagaaccag gaggctgacc ccagccttca gctgcaggag gaggtacaga
     3721 gcctgcgcac cgaactacag aaggctcatt cggagcgcag ggtcctagag gatactcaca
     3781 acagggagaa tggtgaactg agaaagcgag tcgcagacct ggaacatgaa aatgcactct
     3841 tgaaggatga gaaagaacac cttaacaacc aaatcctgcg ccagtcaaaa gctgaatctt
     3901 cacagagctc tgtggaggaa aacctgctga ttaagaagga actggaggag gaacggtccc
     3961 ggtaccagaa cctcgtgaag gagtactccc agctggagca gagatatgag aaccttcggg
     4021 acgagcagca aactccaggc cacaggaaga acccatcaaa tcaaagtagc ttagaatctg
     4081 actccaatta cccctccatt tccacctcag aaatcggaga caccgaggat gccctacagc
     4141 aggtggagga gattggtata gagaaggcag ccatggacat gactgtcttc ttgaagctgc
     4201 agaagagagt gcgggaactt gagcaggaga ggaagaagct gcaggtgcag ctagaaaagg
     4261 aacaacagga cagcaagaaa gtgcaggtag aacaacaaaa caatggccta gatgtggacc
     4321 aggatgcaga tatagcctac aatagtctga agagacaaga gctcgagtca gagaacaaga
     4381 agctgaagaa tgatctgaat gagctgcgga aggctgtagc cgaccaagcc atgcaggata
     4441 actccactca tagctccccc gacagctaca gcctcctact gaaccagctc aagctggcca
     4501 acgaggagct ggaggtccgc aaagaggagg tgctgatcct caggacccag atcatgaatg
     4561 ctgaccagcg cagactgtcc ggcaagaaca tggagccgaa catcaatgcc agaacaagtt
     4621 ggcccaacag cgagaagcac gtggaccagg aagatgccat tgaggcctat cacggggtct
     4681 gccagacaaa cagccagact gaggattggg gatatttgaa tgaagatgga gaactcggct
     4741 tggcttacca aggcctaaag caagtcgcca ggttgctgga ggcccagctg caggcccaga
     4801 acctgaagca tgaggaggag gtggagcatc tcaaggccca ggtggaggcc atgaaggagg
     4861 agatggacaa acagcagcag accttctgcc agactctgct gctctcccca gaggcccagg
     4921 tagaatttgg tgtccagcag gagatatccc ggctgaccaa tgagaacctg gactttaagg
     4981 agttggtgga aaagctggag aagaatgaga agaagctgaa aaagcagctg aagatttaca
     5041 tgaagaaggt ccaggactta gaagctgccc aggcattggc acagagtgac aggaggcacc
     5101 atgaactcac aagacaggtc acggtccaac gaaaagagaa ggacttccaa ggcatgctgg
     5161 agtaccacaa agaggacgag gcactcctta tccggaacct ggtgacagac ctgaagcccc
     5221 agatgctgtc gggcaccgtg ccctgtctgc ctgcatacat actctacatg tgcatcaggc
     5281 acgcggacta caccaacgat gacctcaagg tgcactcgtt gctgagctcc accatcaatg
     5341 gcattaagaa agtcctcaag aagcacaatg aggactttga gatgacgtca ttctggttat
     5401 ccaacacctg ccgcctcctt cactgtttga agcagtacag tggggatgag ggtttcatga
     5461 cacagaacac ggcgaagcag aatgagcact gtctcaagaa ctttgacctc actgaatacc
     5521 gtcaggtgct aagtgacctt tccattcaga tctatcagca actcattaaa attgctgagg
     5581 gcctgctaca gcctatgata gtttctgcca tgctggaaaa tgagagtatc caggggctgt
     5641 caggggtgag gccaactggc taccggaagc gctcgtccag catggtggac ggagaaaatt
     5701 cgtattgcct ggaggccatc atccgccaga tgaatttctt tcatacagtc ctgtgtgacc
     5761 agggcctgga ccctgagatt atcctgcagg tgttcaaaca gcttttctac atgatcaatg
     5821 cggtgactct taacaaccta ctcctgcgga aagatgcctg ctcctggagc accggcatgc
     5881 aactcaggta caacataagt caactcgaag agtggcttcg gggaaaaaac cttcagcaga
     5941 gtggagcggt tcagaccatg gagcccttga tccaggcagc ccaactcctt cagctgaaga
     6001 agaagaccca ggaggatgct gaggccatct gctctctgtg tacctccctc agcacccagc
     6061 agattgtcaa aattttaaac ctctacactc ccttgaatga atttgaagaa cgggtgacag
     6121 tgtcctttat acgaacaatc caggctcagc tacaagagag gagtgacccc cagcagctac
     6181 tgctggactc caaacacatg tttcctgttc tgtttccatt taatccatct gctctgacca
     6241 tggactcaat ccacatcccg gcctgtctca atctggagtt cctcaatgaa gtcggatccg
     6301 actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg
     6361 acaagtagtg agggcccgat atctcgacaa tcaacctctg gattacaaaa tttgtgaaag
     6421 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6481 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6541 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6601 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6661 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6721 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6781 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6841 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6901 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6961 ttgggccgcc tccccgcctg gaaacggggg aggctaactg aaacacggaa ggagacaata
     7021 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
     7081 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     7141 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     7201 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga
     7261 tctgcgcagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     7321 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     7381 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     7441 aaatcggggc atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     7501 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     7561 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     7621 caaccctatc tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg
     7681 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     7741 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7801 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7861 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7921 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7981 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     8041 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     8101 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     8161 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     8221 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     8281 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     8341 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     8401 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     8461 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     8521 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     8581 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     8641 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     8701 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     8761 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     8821 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     8881 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     8941 tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     9001 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     9061 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     9121 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9181 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     9241 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9301 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9361 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9421 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9481 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9541 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9601 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9661 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9721 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9781 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9841 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     9901 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9961 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10021 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10081 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    10141 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10201 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10261 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10321 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10381 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10441 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10501 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10561 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    10621 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    10681 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10741 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10801 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10861 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10921 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10981 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11041 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11101 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11161 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11221 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11281 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11341 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    11401 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
    11461 tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
    11521 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
    11581 ttgaccgaca attgcatgaa gaatctgctt agg
//
