LOCUS       V031142                10698 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031142
VERSION     V031142
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..6845
                     /label="Myh4(NM_010855)"
                     /note="Myh4(NM_010855)"
                     /gene="Myh4"
     CDS             6864..6893
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             6912..6935
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(6955..6971)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    6982..7604
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(7753..8341)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    8670..8717
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(8949..9743)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(9778..10135)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(10137..10241)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      10268..10696
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gagttccgat gctgagatgg ccgtttttgg ggaggctgct ccttacctcc
     1081 ggaagtctga aaaggagcga atcgaggccc agaacaagcc ttttgatgcc aagtcatcgg
     1141 tgtttgtggt ggatgctaag gagtcctatg tgaaagcaac agtgcagagc agggaagggg
     1201 ggaaggtgac agccaagacc gaaggcggag ctacggtcac cgtcaaggat gatcaagtct
     1261 tctccatgaa ccctcccaag tacgacaaga tcgaggacat ggccatgatg acccacctgc
     1321 acgagcccgc tgtgctgtac aacctcaaag agcgttatgc agcctggatg atctacactt
     1381 actcaggcct cttttgtgtc accgtcaacc cctacaagtg gctgccggtg tacaaccctg
     1441 aggtggtggc cgcctacaga ggcaaaaagc gccaggaggc cccaccccac atcttctcca
     1501 tctctgataa cgcctaccag ttcatgctga cggatcgtga gaatcagtcc atcctgatta
     1561 cgggagaatc cggggccggg aagactgtga acacgaagcg tgtcatccag tactttgcaa
     1621 caattgcagt cactggggac aagaagaagg aggaggcgac ttctggcaaa atgcagggga
     1681 cccttgaaga tcaaattatc agtgccaacc ccctgctgga ggcctttggc aacgccaaga
     1741 ctgtgaggaa tgacaactct tctcgatttg gtaaattcat cagaatccat ttcggtgcca
     1801 caggcaaact ggcctctgca gatattgaaa cctatctgct agagaagtcc agagtcacct
     1861 tccagctcaa ggctgaaagg agctaccaca tcttctacca aatcatgtcc aataagaaac
     1921 cagagcttat tgagatgctt ctgatcacca ccaacccata tgactttgct tacgtcagtc
     1981 aaggtgaaat cacagtgccc agcattgatg accaggaaga gctgatggcc acagacactg
     2041 ctgttgatat cctgggattc agtgcagatg aaaaggtggc catttacaag ctcacaggcg
     2101 ctgtgatgca ttatgggaac atgaaattca agcaaaagca aagggaagag caggctgagc
     2161 cagacggcac cgaagttgct gacaaggctg cctatctgac aagtctgaac tctgctgacc
     2221 tgctcaaagc cctctgctac cccagggtca aggtcggcaa tgagtatgtc accaaaggcc
     2281 agacggtgca gcaggtgtac aactccgtgg gtgccctggc caagtccatg tatgagaaga
     2341 tgttcctatg gatggtcacc cgcatcaacc agcagctgga caccaagcag ccccggcagt
     2401 acttcatcgg ggtcttggac attgctggct ttgagatctt tgacttcaac accttggagc
     2461 agctgtgcat caacttcacc aatgagaaac tgcaacagtt tttcaaccac cacatgttcg
     2521 tgctggagca ggaggagtac aagaaggagg gcattgattg ggagttcatt gacttcggga
     2581 tggacctggc ggcctgcatc gagctcatcg agaagccgat gggcatcttc tccatcctgg
     2641 aagaggagtg catgttccct aaggcgacag acacctcctt caagaacaag ctgtatgagc
     2701 agcatcttgg aaagtccaac aacttccaga agcccaaacc cgccaaaggc aaggctgagg
     2761 ctcacttctc cctggtgcac tatgcgggca ccgtggacta caacattatt ggctggctgg
     2821 acaagaacaa ggaccccctg aatgagaccg tggtggggct gtaccagaaa tccgggttga
     2881 agactctggc tttcctattt tctgggggac aagctgcgga agcagagggc ggcggtggaa
     2941 agaaaggtgg caagaagaag ggttcttctt tccagaccgt gtcagctctc ttcagggaga
     3001 atttaaataa gctgatgacc aacttgaaga gcacccaccc ccactttgtc agatgcctca
     3061 ttcccaatga aactaagact cctggtgcca tggagcatga actggtcttg caccagctca
     3121 ggtgtaacgg ggtgctggaa ggcatccgca tctgtaggaa ggggttcccc agcaggatcc
     3181 tctatgcaga cttcaagcag agatacaaag tcctgaatgc aagtgccatc cctgagggtc
     3241 agttcattga tagcaagaag gcctctgaga aacttctggg ttccattgac attgaccaca
     3301 ctcagtacaa attcggtcac actaaggttt tctttaaagc cggcctgttg ggaactctag
     3361 aggagatgcg agatgaaaag ctggctcagc tcatcacccg cactcaagcc gtgtgcagag
     3421 ggtacctgat gagggtggag ttcaaaaaga tgatggagag gagagagtcc atcttctgca
     3481 ttcagtacaa cgtccgcgcc ttcatgaatg tgaaacactg gccctggatg aagctgtatt
     3541 tcaagatcaa acctctgctg aagagcgcgg agaccgagaa ggagatggcc aacatgaagg
     3601 aagattttga gaaggccaag gaggatctgg ctaagtcaga ggcaaaaagg aaggaactag
     3661 aagaaaagat ggtagctttg atgcaggaga aaaatgacct gcagctccag gttcaagctg
     3721 aagcagatgg cttggctgat gctgaggaaa ggtgcgacca gctgatcaaa accaaaatcc
     3781 agctggaggc caaaatcaaa gagctgactg agagggccga ggatgaagag gagatcaacg
     3841 cggagctcac ggccaagaag aggaagctgg aggacgagtg ctcagagctg aagaaggaca
     3901 ttgacgacct tgagctgaca ctggccaagg ttgagaagga gaagcacgcc acggagaaca
     3961 aggtgaaaaa cctgacagaa gagatggcag gcctggatga aaatatcgcc aagctgacca
     4021 aggagaagaa ggccctccag gaggcccacc agcagaccct ggatgacctg caggcagagg
     4081 aggacaaagt caacaccctg accaaagcca aaaccaagct ggaacagcaa gtggatgacc
     4141 ttgaaggatc actggaacag gaaaagaagc tgaggatgga cttagaaaga gccaagagga
     4201 aactggaggg ggacctgaaa ctggcccaag agtccacaat ggacatagaa aacgataaac
     4261 agcagctcga tgagaaactc aaaaagaaag agtttgaaat gagcaatctg cagagcaaga
     4321 ttgaggacga gcaggccctc ggcatgcagc tgcagaagaa gatcaaggag ttgcaggccc
     4381 gcatcgagga gctggaggag gaaatcgagg cagagcgggc ctccagggct aaagcagaga
     4441 agcagcgctc tgacctctcc cgggaactgg aggagatcag cgagaggctg gaagaagctg
     4501 gcggagccac ttcagcccag atcgagatga acaagaagag agaggctgag ttccagaaaa
     4561 tgcgcaggga cctggaggag gccacgctgc agcatgaagc cacagcagct gctcttcgga
     4621 agaagcacgc ggacagcgtg gctgagctcg gggagcagat cgacaacctg cagcgggtga
     4681 agcagaagct ggagaaggag aagagcgagc tgaagatgga gatcgatgat ctcgctagta
     4741 acatggagac agtctccaaa gccaagggga accttgagaa gatgtgccgc accctggagg
     4801 accaactgag tgaagtgaag accaaggagg aggagcagca gcgcctgatc aatgaactgt
     4861 caacccagaa ggcacgcttg cacacagagt caggcgagtt ttcccgacag ctggatgaaa
     4921 aagacgctat ggtttctcag ctttcccgag gcaaacaagc atttacacaa cagattgagg
     4981 aactaaagag gcagctagag gaagagtcca aggccaagaa cgcactggcc cacgccctgc
     5041 agtcagctcg ccatgactgt gacctgctgc gggaacagta tgaagaggag caggaagcca
     5101 aggctgagct gcagagggcc atgtccaagg ccaacagcga ggtagcccag tggaggacga
     5161 aatatgagac ggatgccatc cagcgcacag aggagctgga agaggccaag aagaagctgg
     5221 ctcagcgtct gcaggatgct gaggaacatg tagaagctgt gaattccaaa tgcgcttctc
     5281 ttgaaaagac gaagcagcga cttcagaatg aagtggagga cctcatgatt gacgtggaga
     5341 ggtctaatgc tgcctgcgct gcgcttgata agaagcagag gaactttgac aaggtcctgg
     5401 cagaatggaa acagaagtac gaggaaactc aggctgagct tgaagcctcc cagaaggagt
     5461 cccgctctct gagcactgag ctgttcaaag tgaagaatgc ctatgaggag tctctggatc
     5521 aacttgagac gttaaagaga gagaacaaga acctgcagca ggagatctct gacctgaccg
     5581 agcagattgc tgagggaggg aagcacatcc atgagctgga gaaaatcaag aagcaaattg
     5641 atcaggaaaa gagtgaatta caggcttctc tggaggaagc ggaggcatcc cttgaacatg
     5701 aagaaggtaa aatcctacgc atccagctgg agttgaacca ggtgaaatct gagattgacc
     5761 gcaaaattgc tgaaaaagat gaagagatag atcagctgaa gagaaaccac ctcagagttg
     5821 tggagtccat gcagagcacg ctggacgccg agatccgcag caggaatgat gctctgagga
     5881 ttaagaagaa gatggaggga gacctcaatg agatggagat ccagctgaac catgccaacc
     5941 gccaggctgc ggaggcaatc aggaaccttc ggaacaccca gggaatgctg aaggacacac
     6001 agctgcacct ggacgatgct ctcagaggcc aggacgacct gaaagagcag ctggccatgg
     6061 ttgagcgcag agccaacctg atgcaggctg agatcgagga gctcagggca tccctggaac
     6121 agacagagag gagcaggaga gtggccgagc aagagctact ggatgccagt gagcgcgtgc
     6181 agctcctcca cacccagaac accagcctca tcaacaccaa gaagaagctg gagacagaca
     6241 tttcccaaat ccagggagag atggaagaca tcgtccagga agcccgcaac gcagaagaga
     6301 aggccaagaa agccatcact gatgccgcca tgatggcgga ggagctgaag aaggagcagg
     6361 acaccagcgc ccacctggag cggatgaaga agaacatgga gcagaccgtg aaggacctgc
     6421 agcaccgtct ggacgaggct gagcagctgg cgctgaaggg cggcaagaag cagatccaga
     6481 aactggaggc tagggtgagg gagcttgaaa acgaggtgga aaatgaacag aagcgcaaca
     6541 tcgaagccgt caagggtctt cgtaagcacg agcgcagagt gaaggaactc acctaccaga
     6601 ccgaggagga ccgcaagaac gtgctgaggc tgcaggactt ggtggacaaa ctacagacta
     6661 aagtgaaagc ctacaagaga caggctgagg aggctgagga acaatccaat gtcaacctgg
     6721 ccaagttccg taagatccag cacgagctgg aggaagccga ggagcgggct gacatcgcgg
     6781 agtcccaggt caacaagctg cgggtgaaga gccgagaggt tcacactaaa gtcataagcg
     6841 aagaaacgcg tacgcggccg ctcgagcaga aactcatctc agaagaggat ctggcagcaa
     6901 atgatatcct ggattacaag gatgacgacg ataaggttta aacggccggc cgcggtcata
     6961 gctgtttcct gaacagatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     7021 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     7081 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     7141 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     7201 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     7261 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     7321 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     7381 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     7441 gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca
     7501 cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact
     7561 gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagcg
     7621 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7681 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7741 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7801 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7861 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7921 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7981 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8041 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8101 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8161 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8221 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8281 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8341 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8401 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8461 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc
     8521 cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga
     8581 aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg
     8641 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt
     8701 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     8761 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
     8821 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
     8881 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
     8941 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     9001 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     9061 atatcacggg tagccaacgc tatgtcctga tagcgatccg ccacacccag ccggccacag
     9121 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     9181 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
     9241 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     9301 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     9361 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     9421 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     9481 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     9541 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
     9601 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     9661 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     9721 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt
     9781 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     9841 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     9901 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     9961 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
    10021 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
    10081 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
    10141 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10201 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10261 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
    10321 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
    10381 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
    10441 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    10501 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    10561 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
    10621 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
    10681 aaatttaacg cgaatttt
//
